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The significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease

We aimed to determine the significance of cytoplasmic antinuclear antibody (ANA) patterns using computer-aided immunofluorescence microscopy in patients with autoimmune liver diseases (AILD). ANA staining pattern was identified by treating cultured human epithelial type 2 (HEp-2) cells with the sera...

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Autores principales: Cha, Hyun Jin, Hwang, Jimin, Lee, Lucy Eunju, Park, Younhee, Song, Jason Jungsik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790257/
https://www.ncbi.nlm.nih.gov/pubmed/33411840
http://dx.doi.org/10.1371/journal.pone.0244950
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author Cha, Hyun Jin
Hwang, Jimin
Lee, Lucy Eunju
Park, Younhee
Song, Jason Jungsik
author_facet Cha, Hyun Jin
Hwang, Jimin
Lee, Lucy Eunju
Park, Younhee
Song, Jason Jungsik
author_sort Cha, Hyun Jin
collection PubMed
description We aimed to determine the significance of cytoplasmic antinuclear antibody (ANA) patterns using computer-aided immunofluorescence microscopy in patients with autoimmune liver diseases (AILD). ANA staining pattern was identified by treating cultured human epithelial type 2 (HEp-2) cells with the sera of the patients. Medical records of patients with suspected AILD who had positive cytoplasmic ANA patterns between February 2017 and November 2019 were retrospectively reviewed for clinical, laboratory, and immunological data. Cytoplasmic ANA patterns of AILD and non-AILD groups were compared. Further subgroup analysis of patients with AILD who had reticular or speckled cytoplasmic ANA patterns was conducted. We found that among the 196 patients with positive cytoplasmic ANA patterns, 113 (57.6%) were diagnosed with AILD. The percentage of reticular cytoplasmic pattern was higher in the AILD group than that in the non-AILD group (64.0% vs. 21.9%, p < 0.001). Furthermore, patients with AILD who exhibited a reticular ANA pattern demonstrated a higher positive rate for anti-mitochondrial antibodies (66.7% vs. 2.6%, p < 0.001) than those who exhibited the speckled ANA pattern. Moreover, AILD patients with the reticular ANA pattern displayed a lower positive rate for anti-smooth muscle antibodies (0% vs. 45%, p < 0.001) and nuclear ANA pattern (73.2% vs. 97.5%, p = 0.003) than those with the speckled ANA pattern. Therefore, cytoplasmic ANA patterns could be used to guide AILD characterization in suspected AILD cases, especially as the reticular ANA pattern is strongly associated with AILD. Thus, it is important to check cytoplasmic ANA patterns for AILD evaluation, even when nuclear ANA patterns are negative.
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spelling pubmed-77902572021-01-14 The significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease Cha, Hyun Jin Hwang, Jimin Lee, Lucy Eunju Park, Younhee Song, Jason Jungsik PLoS One Research Article We aimed to determine the significance of cytoplasmic antinuclear antibody (ANA) patterns using computer-aided immunofluorescence microscopy in patients with autoimmune liver diseases (AILD). ANA staining pattern was identified by treating cultured human epithelial type 2 (HEp-2) cells with the sera of the patients. Medical records of patients with suspected AILD who had positive cytoplasmic ANA patterns between February 2017 and November 2019 were retrospectively reviewed for clinical, laboratory, and immunological data. Cytoplasmic ANA patterns of AILD and non-AILD groups were compared. Further subgroup analysis of patients with AILD who had reticular or speckled cytoplasmic ANA patterns was conducted. We found that among the 196 patients with positive cytoplasmic ANA patterns, 113 (57.6%) were diagnosed with AILD. The percentage of reticular cytoplasmic pattern was higher in the AILD group than that in the non-AILD group (64.0% vs. 21.9%, p < 0.001). Furthermore, patients with AILD who exhibited a reticular ANA pattern demonstrated a higher positive rate for anti-mitochondrial antibodies (66.7% vs. 2.6%, p < 0.001) than those who exhibited the speckled ANA pattern. Moreover, AILD patients with the reticular ANA pattern displayed a lower positive rate for anti-smooth muscle antibodies (0% vs. 45%, p < 0.001) and nuclear ANA pattern (73.2% vs. 97.5%, p = 0.003) than those with the speckled ANA pattern. Therefore, cytoplasmic ANA patterns could be used to guide AILD characterization in suspected AILD cases, especially as the reticular ANA pattern is strongly associated with AILD. Thus, it is important to check cytoplasmic ANA patterns for AILD evaluation, even when nuclear ANA patterns are negative. Public Library of Science 2021-01-07 /pmc/articles/PMC7790257/ /pubmed/33411840 http://dx.doi.org/10.1371/journal.pone.0244950 Text en © 2021 Cha et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cha, Hyun Jin
Hwang, Jimin
Lee, Lucy Eunju
Park, Younhee
Song, Jason Jungsik
The significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease
title The significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease
title_full The significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease
title_fullStr The significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease
title_full_unstemmed The significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease
title_short The significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease
title_sort significance of cytoplasmic antinuclear antibody patterns in autoimmune liver disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790257/
https://www.ncbi.nlm.nih.gov/pubmed/33411840
http://dx.doi.org/10.1371/journal.pone.0244950
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