Cargando…
Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway
Liver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790590/ https://www.ncbi.nlm.nih.gov/pubmed/33488687 http://dx.doi.org/10.1155/2020/6694214 |
_version_ | 1783633458199789568 |
---|---|
author | Xia, Yujing Li, Jingjing Chen, Kan Feng, Jiao Guo, Chuanyong |
author_facet | Xia, Yujing Li, Jingjing Chen, Kan Feng, Jiao Guo, Chuanyong |
author_sort | Xia, Yujing |
collection | PubMed |
description | Liver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by carbon tetrachloride and bile duct ligation. A variety of molecular biological methods (qRT-PCR, western blotting, and immunohistochemistry) were employed to confirm the increased degree of hepatocyte injury and ECM formation in the disease model, consistent with autophagy and activation of the TGF-β pathway. Bergenin activated PPAR-γ and inhibited TGF-β and autophagy and decreased liver fibrosis by inhibiting hepatocyte necrosis and ECM formation in a dose-dependent manner. The results suggest that bergenin may be a promising drug candidate for the treatment of liver fibrosis. |
format | Online Article Text |
id | pubmed-7790590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-77905902021-01-21 Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway Xia, Yujing Li, Jingjing Chen, Kan Feng, Jiao Guo, Chuanyong PPAR Res Research Article Liver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by carbon tetrachloride and bile duct ligation. A variety of molecular biological methods (qRT-PCR, western blotting, and immunohistochemistry) were employed to confirm the increased degree of hepatocyte injury and ECM formation in the disease model, consistent with autophagy and activation of the TGF-β pathway. Bergenin activated PPAR-γ and inhibited TGF-β and autophagy and decreased liver fibrosis by inhibiting hepatocyte necrosis and ECM formation in a dose-dependent manner. The results suggest that bergenin may be a promising drug candidate for the treatment of liver fibrosis. Hindawi 2020-12-31 /pmc/articles/PMC7790590/ /pubmed/33488687 http://dx.doi.org/10.1155/2020/6694214 Text en Copyright © 2020 Yujing Xia et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xia, Yujing Li, Jingjing Chen, Kan Feng, Jiao Guo, Chuanyong Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
title | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
title_full | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
title_fullStr | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
title_full_unstemmed | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
title_short | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
title_sort | bergenin attenuates hepatic fibrosis by regulating autophagy mediated by the ppar-γ/tgf-β pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790590/ https://www.ncbi.nlm.nih.gov/pubmed/33488687 http://dx.doi.org/10.1155/2020/6694214 |
work_keys_str_mv | AT xiayujing bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway AT lijingjing bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway AT chenkan bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway AT fengjiao bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway AT guochuanyong bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway |