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Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway

Liver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by...

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Detalles Bibliográficos
Autores principales: Xia, Yujing, Li, Jingjing, Chen, Kan, Feng, Jiao, Guo, Chuanyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790590/
https://www.ncbi.nlm.nih.gov/pubmed/33488687
http://dx.doi.org/10.1155/2020/6694214
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author Xia, Yujing
Li, Jingjing
Chen, Kan
Feng, Jiao
Guo, Chuanyong
author_facet Xia, Yujing
Li, Jingjing
Chen, Kan
Feng, Jiao
Guo, Chuanyong
author_sort Xia, Yujing
collection PubMed
description Liver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by carbon tetrachloride and bile duct ligation. A variety of molecular biological methods (qRT-PCR, western blotting, and immunohistochemistry) were employed to confirm the increased degree of hepatocyte injury and ECM formation in the disease model, consistent with autophagy and activation of the TGF-β pathway. Bergenin activated PPAR-γ and inhibited TGF-β and autophagy and decreased liver fibrosis by inhibiting hepatocyte necrosis and ECM formation in a dose-dependent manner. The results suggest that bergenin may be a promising drug candidate for the treatment of liver fibrosis.
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spelling pubmed-77905902021-01-21 Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway Xia, Yujing Li, Jingjing Chen, Kan Feng, Jiao Guo, Chuanyong PPAR Res Research Article Liver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by carbon tetrachloride and bile duct ligation. A variety of molecular biological methods (qRT-PCR, western blotting, and immunohistochemistry) were employed to confirm the increased degree of hepatocyte injury and ECM formation in the disease model, consistent with autophagy and activation of the TGF-β pathway. Bergenin activated PPAR-γ and inhibited TGF-β and autophagy and decreased liver fibrosis by inhibiting hepatocyte necrosis and ECM formation in a dose-dependent manner. The results suggest that bergenin may be a promising drug candidate for the treatment of liver fibrosis. Hindawi 2020-12-31 /pmc/articles/PMC7790590/ /pubmed/33488687 http://dx.doi.org/10.1155/2020/6694214 Text en Copyright © 2020 Yujing Xia et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xia, Yujing
Li, Jingjing
Chen, Kan
Feng, Jiao
Guo, Chuanyong
Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway
title Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway
title_full Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway
title_fullStr Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway
title_full_unstemmed Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway
title_short Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway
title_sort bergenin attenuates hepatic fibrosis by regulating autophagy mediated by the ppar-γ/tgf-β pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790590/
https://www.ncbi.nlm.nih.gov/pubmed/33488687
http://dx.doi.org/10.1155/2020/6694214
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