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Microbiota-dependent expansion of testicular IL-17-producing Vγ6(+) γδ T cells upon puberty promotes local tissue immune surveillance
γδT cells represent the majority of lymphocytes in several mucosal tissues where they contribute to tissue homoeostasis, microbial defence and wound repair. Here we characterise a population of interleukin (IL) 17-producing γδ (γδ17) T cells that seed the testis of naive C57BL/6 mice, expand at pube...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790758/ https://www.ncbi.nlm.nih.gov/pubmed/32733025 http://dx.doi.org/10.1038/s41385-020-0330-6 |
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author | Wilharm, Anneke Brigas, Helena C. Sandrock, Inga Ribeiro, Miguel Amado, Tiago Reinhardt, Annika Demera, Abdi Hoenicke, Lisa Strowig, Till Carvalho, Tânia Prinz, Immo Ribot, Julie C. |
author_facet | Wilharm, Anneke Brigas, Helena C. Sandrock, Inga Ribeiro, Miguel Amado, Tiago Reinhardt, Annika Demera, Abdi Hoenicke, Lisa Strowig, Till Carvalho, Tânia Prinz, Immo Ribot, Julie C. |
author_sort | Wilharm, Anneke |
collection | PubMed |
description | γδT cells represent the majority of lymphocytes in several mucosal tissues where they contribute to tissue homoeostasis, microbial defence and wound repair. Here we characterise a population of interleukin (IL) 17-producing γδ (γδ17) T cells that seed the testis of naive C57BL/6 mice, expand at puberty and persist throughout adulthood. We show that this population is foetal-derived and displays a T-cell receptor (TCR) repertoire highly biased towards Vγ6-containing rearrangements. These γδ17 cells were the major source of IL-17 in the testis, whereas αβ T cells mostly provided interferon (IFN)-γ in situ. Importantly, testicular γδ17 cell homoeostasis was strongly dependent on the microbiota and Toll-like receptor (TLR4)/IL-1α/IL-23 signalling. We further found that γδ17 cells contributed to tissue surveillance in a model of experimental orchitis induced by intra-testicular inoculation of Listeria monocytogenes, as Tcrδ(−/−) and Il17(−/−) infected mice displayed higher bacterial loads than wild-type (WT) controls and died 3 days after infection. Altogether, this study identified a previously unappreciated foetal-derived γδ17 cell subset that infiltrates the testis at steady state, expands upon puberty and plays a crucial role in local tissue immune surveillance. |
format | Online Article Text |
id | pubmed-7790758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group US |
record_format | MEDLINE/PubMed |
spelling | pubmed-77907582021-01-15 Microbiota-dependent expansion of testicular IL-17-producing Vγ6(+) γδ T cells upon puberty promotes local tissue immune surveillance Wilharm, Anneke Brigas, Helena C. Sandrock, Inga Ribeiro, Miguel Amado, Tiago Reinhardt, Annika Demera, Abdi Hoenicke, Lisa Strowig, Till Carvalho, Tânia Prinz, Immo Ribot, Julie C. Mucosal Immunol Article γδT cells represent the majority of lymphocytes in several mucosal tissues where they contribute to tissue homoeostasis, microbial defence and wound repair. Here we characterise a population of interleukin (IL) 17-producing γδ (γδ17) T cells that seed the testis of naive C57BL/6 mice, expand at puberty and persist throughout adulthood. We show that this population is foetal-derived and displays a T-cell receptor (TCR) repertoire highly biased towards Vγ6-containing rearrangements. These γδ17 cells were the major source of IL-17 in the testis, whereas αβ T cells mostly provided interferon (IFN)-γ in situ. Importantly, testicular γδ17 cell homoeostasis was strongly dependent on the microbiota and Toll-like receptor (TLR4)/IL-1α/IL-23 signalling. We further found that γδ17 cells contributed to tissue surveillance in a model of experimental orchitis induced by intra-testicular inoculation of Listeria monocytogenes, as Tcrδ(−/−) and Il17(−/−) infected mice displayed higher bacterial loads than wild-type (WT) controls and died 3 days after infection. Altogether, this study identified a previously unappreciated foetal-derived γδ17 cell subset that infiltrates the testis at steady state, expands upon puberty and plays a crucial role in local tissue immune surveillance. Nature Publishing Group US 2020-07-30 2021 /pmc/articles/PMC7790758/ /pubmed/32733025 http://dx.doi.org/10.1038/s41385-020-0330-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wilharm, Anneke Brigas, Helena C. Sandrock, Inga Ribeiro, Miguel Amado, Tiago Reinhardt, Annika Demera, Abdi Hoenicke, Lisa Strowig, Till Carvalho, Tânia Prinz, Immo Ribot, Julie C. Microbiota-dependent expansion of testicular IL-17-producing Vγ6(+) γδ T cells upon puberty promotes local tissue immune surveillance |
title | Microbiota-dependent expansion of testicular IL-17-producing Vγ6(+) γδ T cells upon puberty promotes local tissue immune surveillance |
title_full | Microbiota-dependent expansion of testicular IL-17-producing Vγ6(+) γδ T cells upon puberty promotes local tissue immune surveillance |
title_fullStr | Microbiota-dependent expansion of testicular IL-17-producing Vγ6(+) γδ T cells upon puberty promotes local tissue immune surveillance |
title_full_unstemmed | Microbiota-dependent expansion of testicular IL-17-producing Vγ6(+) γδ T cells upon puberty promotes local tissue immune surveillance |
title_short | Microbiota-dependent expansion of testicular IL-17-producing Vγ6(+) γδ T cells upon puberty promotes local tissue immune surveillance |
title_sort | microbiota-dependent expansion of testicular il-17-producing vγ6(+) γδ t cells upon puberty promotes local tissue immune surveillance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790758/ https://www.ncbi.nlm.nih.gov/pubmed/32733025 http://dx.doi.org/10.1038/s41385-020-0330-6 |
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