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Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice

BACKGROUND: The circadian rhythm is the internal clock that controls sleep-wake cycles, metabolism, cognition, and several processes in the body, and its disruption has been associated with aging. The differentiated embryo chondrocyte (Dec) gene is related to circadian rhythm. To our knowledge, ther...

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Autores principales: Nam, Youn Hee, Jeong, Seo Yule, Kim, Yun Hee, Rodriguez, Isabel, Nuankaew, Wanlapa, Bhawal, Ujjal K., Hong, Bin Na, Kang, Tong Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790900/
https://www.ncbi.nlm.nih.gov/pubmed/33437170
http://dx.doi.org/10.1016/j.jgr.2020.09.003
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author Nam, Youn Hee
Jeong, Seo Yule
Kim, Yun Hee
Rodriguez, Isabel
Nuankaew, Wanlapa
Bhawal, Ujjal K.
Hong, Bin Na
Kang, Tong Ho
author_facet Nam, Youn Hee
Jeong, Seo Yule
Kim, Yun Hee
Rodriguez, Isabel
Nuankaew, Wanlapa
Bhawal, Ujjal K.
Hong, Bin Na
Kang, Tong Ho
author_sort Nam, Youn Hee
collection PubMed
description BACKGROUND: The circadian rhythm is the internal clock that controls sleep-wake cycles, metabolism, cognition, and several processes in the body, and its disruption has been associated with aging. The differentiated embryo chondrocyte (Dec) gene is related to circadian rhythm. To our knowledge, there are no reports of the relationship between dec gene expression and KRG effect. Therefore, we treated Dec gene knockout (KO) aging mice with KRG to study anti-aging related effects and possible mechanisms. METHODS: We evaluated KRG and expression of Dec genes in an ototoxicity model. Dec genes expression in livers of aging mice was further analyzed. Then, we assessed the effects of DEC KO on hearing function in mice by ABR. Finally, we performed DNA microarray to identify KRG-related gene expression changes in mouse liver and assessed the results using KEGG analysis. RESULTS: KRG decreased the expression of Dec genes in ototoxicity model, which may contribute to its anti-aging efficacy. Moreover, KRG suppressed Dec genes expression in liver of wild type indicating inhibition of senescence. ABR test indicated that KRG improved auditory function in aging mouse, demonstrating KRG efficacy on aging related diseases. CONCLUSION: Finally, in KEGG analysis of 238 genes that were activated and 158 that were inhibited by KRG in DEC KO mice, activated genes were involved in proliferation signaling, mineral absorption, and PPAR signaling whereas the inhibited genes were involved in arachidonic acid metabolism and peroxisomes. Our data indicate that inhibition of senescence-related Dec genes may explain the anti-aging efficacy of KRG.
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spelling pubmed-77909002021-01-11 Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice Nam, Youn Hee Jeong, Seo Yule Kim, Yun Hee Rodriguez, Isabel Nuankaew, Wanlapa Bhawal, Ujjal K. Hong, Bin Na Kang, Tong Ho J Ginseng Res Research Article BACKGROUND: The circadian rhythm is the internal clock that controls sleep-wake cycles, metabolism, cognition, and several processes in the body, and its disruption has been associated with aging. The differentiated embryo chondrocyte (Dec) gene is related to circadian rhythm. To our knowledge, there are no reports of the relationship between dec gene expression and KRG effect. Therefore, we treated Dec gene knockout (KO) aging mice with KRG to study anti-aging related effects and possible mechanisms. METHODS: We evaluated KRG and expression of Dec genes in an ototoxicity model. Dec genes expression in livers of aging mice was further analyzed. Then, we assessed the effects of DEC KO on hearing function in mice by ABR. Finally, we performed DNA microarray to identify KRG-related gene expression changes in mouse liver and assessed the results using KEGG analysis. RESULTS: KRG decreased the expression of Dec genes in ototoxicity model, which may contribute to its anti-aging efficacy. Moreover, KRG suppressed Dec genes expression in liver of wild type indicating inhibition of senescence. ABR test indicated that KRG improved auditory function in aging mouse, demonstrating KRG efficacy on aging related diseases. CONCLUSION: Finally, in KEGG analysis of 238 genes that were activated and 158 that were inhibited by KRG in DEC KO mice, activated genes were involved in proliferation signaling, mineral absorption, and PPAR signaling whereas the inhibited genes were involved in arachidonic acid metabolism and peroxisomes. Our data indicate that inhibition of senescence-related Dec genes may explain the anti-aging efficacy of KRG. Elsevier 2021-01 2020-09-16 /pmc/articles/PMC7790900/ /pubmed/33437170 http://dx.doi.org/10.1016/j.jgr.2020.09.003 Text en © 2020 The Korean Society of Ginseng. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Nam, Youn Hee
Jeong, Seo Yule
Kim, Yun Hee
Rodriguez, Isabel
Nuankaew, Wanlapa
Bhawal, Ujjal K.
Hong, Bin Na
Kang, Tong Ho
Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice
title Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice
title_full Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice
title_fullStr Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice
title_full_unstemmed Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice
title_short Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice
title_sort anti-aging effects of korean red ginseng (krg) in differentiated embryo chondrocyte (dec) knockout mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790900/
https://www.ncbi.nlm.nih.gov/pubmed/33437170
http://dx.doi.org/10.1016/j.jgr.2020.09.003
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