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enAsCas12a Enables CRISPR-Directed Evolution to Screen for Functional Drug Resistance Mutations in Sequences Inaccessible to SpCas9

While drug resistance mutations provide the gold standard proof for drug target engagement, target deconvolution of inhibitors identified from a phenotypic screen remains challenging. Genetic screening for functional in-frame drug resistance mutations by tiling CRISPR-Cas nucleases across protein co...

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Detalles Bibliográficos
Autores principales:	Neggers, Jasper Edgar, Jacquemyn, Maarten, Dierckx, Tim, Kleinstiver, Benjamin Peter, Thibaut, Hendrik Jan, Daelemans, Dirk
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Gene & Cell Therapy 2021
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791016/ https://www.ncbi.nlm.nih.gov/pubmed/33002419 http://dx.doi.org/10.1016/j.ymthe.2020.09.025

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