Cargando…

Targeted Knockout of the Vegfa Gene in the Retina by Subretinal Injection of RNP Complexes Containing Cas9 Protein and Modified sgRNAs

The therapeutic effect of retinal gene therapy using CRISPR/Cas9-mediated genome editing and knockout applications is dependent on efficient and safe delivery of gene-modifying tool kits. Recently, transient administration of single guide RNAs (sgRNAs) and SpCas9 proteins delivered as ribonucleoprot...

Descripción completa

Detalles Bibliográficos
Autores principales:	Holmgaard, Andreas Braae, Askou, Anne Louise, Jensen, Emilie Grarup, Alsing, Sidsel, Bak, Rasmus O., Mikkelsen, Jacob Giehm, Corydon, Thomas J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Gene & Cell Therapy 2021
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791085/ https://www.ncbi.nlm.nih.gov/pubmed/33022212 http://dx.doi.org/10.1016/j.ymthe.2020.09.032

Ejemplares similares

In Vivo Knockout of the Vegfa Gene by Lentiviral Delivery of CRISPR/Cas9 in Mouse Retinal Pigment Epithelium Cells
por: Holmgaard, Andreas, et al.
Publicado: (2017)

Suppression of Choroidal Neovascularization by AAV-Based Dual-Acting Antiangiogenic Gene Therapy
por: Askou, Anne Louise, et al.
Publicado: (2019)

VEGFA-targeting miR-agshRNAs combine efficacy with specificity and safety for retinal gene therapy
por: Alsing, Sidsel, et al.
Publicado: (2022)

Efficient large fragment deletion in plants: double pairs of sgRNAs are better than dual sgRNAs
por: Zhu, Guoning, et al.
Publicado: (2023)

Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency
por: García-Tuñón, Ignacio, et al.
Publicado: (2019)

CRISPRz: a database of zebrafish validated sgRNAs
por: Varshney, Gaurav K., et al.
Publicado: (2016)

Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing
por: Chen, Zexiang, et al.
Publicado: (2022)

sgRNA-PSM: Predict sgRNAs On-Target Activity Based on Position-Specific Mismatch
por: Liu, Bin, et al.
Publicado: (2020)

Elimination of Specific miRNAs by Naked 14-nt sgRNAs
por: Takahashi, Masayuki, et al.
Publicado: (2012)

CRISPy-web: An online resource to design sgRNAs for CRISPR applications
por: Blin, Kai, et al.
Publicado: (2016)

GRIBCG: a software for selection of sgRNAs in the design of balancer chromosomes
por: Merritt, Brian B., et al.
Publicado: (2019)

sgRNAs: A SARS-CoV-2 emerging issue
por: Mori, Antonio, et al.
Publicado: (2023)

CIDP: a multi-functional platform for designing CRISPR sgRNAs
por: Xu, Dong, et al.
Publicado: (2023)

Animal Models of Choroidal Neovascularization: A Systematic Review
por: Fabian-Jessing, Bjørn K., et al.
Publicado: (2022)

CRISPRlnc: a manually curated database of validated sgRNAs for lncRNAs
por: Chen, Wen, et al.
Publicado: (2019)

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
por: Kundert, Kale, et al.
Publicado: (2019)

Protocol to Design, Clone, and Validate sgRNAs for In Vivo Reverse Genetic Studies
por: Hunker, Avery C., et al.
Publicado: (2020)

One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
por: Zuo, Erwei, et al.
Publicado: (2017)

CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo
por: Moreno-Mateos, Miguel A., et al.
Publicado: (2015)

Artificial sgRNAs engineered for genome editing with new Cas12b orthologs
por: Teng, Fei, et al.
Publicado: (2019)

Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors
por: Zhang, Ao, et al.
Publicado: (2023)

Subretinal Saline Protects the Neuroretina From Thermic Damage During Laser Induction of Experimental Choroidal Neovascularization in Pigs
por: Hansen, Silja, et al.
Publicado: (2021)

CRISPR/Cas9-mediated tissue-specific knockout and cDNA rescue using sgRNAs that target exon-intron junctions in Drosophila melanogaster
por: Chilian, Madison, et al.
Publicado: (2022)

Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing
por: Liang, Gang, et al.
Publicado: (2016)

Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
por: Kim, Sojung, et al.
Publicado: (2017)

Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency
por: Jang, Da Eun, et al.
Publicado: (2018)

Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
por: Hegde, Mudra, et al.
Publicado: (2018)

Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions
por: Blayney, Joseph, et al.
Publicado: (2020)

Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0
por: Blin, Kai, et al.
Publicado: (2020)

Customized optical mapping by CRISPR–Cas9 mediated DNA labeling with multiple sgRNAs
por: Abid, Heba Z, et al.
Publicado: (2020)

Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high‐fidelity Cas9 for precise genome editing in marine algae
por: Sharma, Amit K., et al.
Publicado: (2021)

Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein
por: Dalsgaard, Trine, et al.
Publicado: (2018)

Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification
por: Zhang, Kaixiang, et al.
Publicado: (2016)

PAVOOC: designing CRISPR sgRNAs using 3D protein structures and functional domain annotations
por: Schaefer, Moritz, et al.
Publicado: (2019)

Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay
por: Lyu, Pin, et al.
Publicado: (2020)

Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
por: Iwasaki, Roman S., et al.
Publicado: (2020)

CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs
por: Lee, Ho Joung, et al.
Publicado: (2020)

CRISPR-surfaceome: An online tool for designing highly efficient sgRNAs targeting cell surface proteins
por: Mei, Hong, et al.
Publicado: (2022)

Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in Flammulina filiformis
por: Liu, Xiaotian, et al.
Publicado: (2022)

Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system
por: Shao, Shipeng, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_kmdne14dtplv9c6r2qt5aof66q