Cargando…
Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas
BACKGROUND: HIV infection is a major health problem in Russia. We aimed to assess HIV prevalence in different population groups and to compare the characteristics of 4th generation immunoassays from Abbott, Bio-Rad, Vector-Best, Diagnostic Systems, and Medical Biological Unit. METHODS: The study inc...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791727/ https://www.ncbi.nlm.nih.gov/pubmed/33413197 http://dx.doi.org/10.1186/s12879-020-05695-z |
_version_ | 1783633653721464832 |
---|---|
author | Kireev, D. E. Chulanov, V. P. Shipulin, G. A. Semenov, A. V. Tivanova, E. V. Kolyasnikova, N. M. Zueva, E. B. Pokrovskiy, V. V. Galli, C. |
author_facet | Kireev, D. E. Chulanov, V. P. Shipulin, G. A. Semenov, A. V. Tivanova, E. V. Kolyasnikova, N. M. Zueva, E. B. Pokrovskiy, V. V. Galli, C. |
author_sort | Kireev, D. E. |
collection | PubMed |
description | BACKGROUND: HIV infection is a major health problem in Russia. We aimed to assess HIV prevalence in different population groups and to compare the characteristics of 4th generation immunoassays from Abbott, Bio-Rad, Vector-Best, Diagnostic Systems, and Medical Biological Unit. METHODS: The study included 4452 individuals from the general population (GP), 391 subjects at high risk of HIV infection (HR) and 699 with potentially interfering conditions. HIV positivity was confirmed by immunoblot and by HIV RNA, seroconversion and virus diversity panels were also used. HIV avidity was employed to assess recent infections. RESULTS: The prevalence in GP was 0.40%, higher in males (0.62%) and in people aged < 40 years (0.58%). Patients attending dermo-venereal centers and drug users had a high prevalence (34.1 and 58.8%). Recent infections were diagnosed in 20% of GP and in 4.2% of HR. Assay sensitivity was 100% except for one false negative (99,54%, MBU). Specificity was 99.58–99.89% overall, but as low as 93.26% on HR (Vector-Best). Small differences on early seroconversion were recorded. Only the Abbott assay detected all samples on the viral diversity panel. CONCLUSION: HIV infection rate in the high-risk groups suggests that awareness and screening campaigns should be enhanced. Fourth generation assays are adequate but performance differences must be considered. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-020-05695-z. |
format | Online Article Text |
id | pubmed-7791727 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77917272021-01-11 Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas Kireev, D. E. Chulanov, V. P. Shipulin, G. A. Semenov, A. V. Tivanova, E. V. Kolyasnikova, N. M. Zueva, E. B. Pokrovskiy, V. V. Galli, C. BMC Infect Dis Research Article BACKGROUND: HIV infection is a major health problem in Russia. We aimed to assess HIV prevalence in different population groups and to compare the characteristics of 4th generation immunoassays from Abbott, Bio-Rad, Vector-Best, Diagnostic Systems, and Medical Biological Unit. METHODS: The study included 4452 individuals from the general population (GP), 391 subjects at high risk of HIV infection (HR) and 699 with potentially interfering conditions. HIV positivity was confirmed by immunoblot and by HIV RNA, seroconversion and virus diversity panels were also used. HIV avidity was employed to assess recent infections. RESULTS: The prevalence in GP was 0.40%, higher in males (0.62%) and in people aged < 40 years (0.58%). Patients attending dermo-venereal centers and drug users had a high prevalence (34.1 and 58.8%). Recent infections were diagnosed in 20% of GP and in 4.2% of HR. Assay sensitivity was 100% except for one false negative (99,54%, MBU). Specificity was 99.58–99.89% overall, but as low as 93.26% on HR (Vector-Best). Small differences on early seroconversion were recorded. Only the Abbott assay detected all samples on the viral diversity panel. CONCLUSION: HIV infection rate in the high-risk groups suggests that awareness and screening campaigns should be enhanced. Fourth generation assays are adequate but performance differences must be considered. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-020-05695-z. BioMed Central 2021-01-07 /pmc/articles/PMC7791727/ /pubmed/33413197 http://dx.doi.org/10.1186/s12879-020-05695-z Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Kireev, D. E. Chulanov, V. P. Shipulin, G. A. Semenov, A. V. Tivanova, E. V. Kolyasnikova, N. M. Zueva, E. B. Pokrovskiy, V. V. Galli, C. Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas |
title | Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas |
title_full | Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas |
title_fullStr | Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas |
title_full_unstemmed | Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas |
title_short | Serological diagnosis and prevalence of HIV-1 infection in Russian metropolitan areas |
title_sort | serological diagnosis and prevalence of hiv-1 infection in russian metropolitan areas |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791727/ https://www.ncbi.nlm.nih.gov/pubmed/33413197 http://dx.doi.org/10.1186/s12879-020-05695-z |
work_keys_str_mv | AT kireevde serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas AT chulanovvp serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas AT shipulinga serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas AT semenovav serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas AT tivanovaev serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas AT kolyasnikovanm serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas AT zuevaeb serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas AT pokrovskiyvv serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas AT gallic serologicaldiagnosisandprevalenceofhiv1infectioninrussianmetropolitanareas |