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N-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test

BACKGROUND: Acetaminophen (APAP) induced hepatotoxicity is a clinically important problem. Up to now, interventive therapy with n-acetylcysteine (NAC) has been considered as a gold-standard treatment for APAP overdose. However, no study has focused on the efficacy of these drugs’ concurrent administ...

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Autores principales: Nakhaee, Samaneh, Dastjerdi, Mohammad, Roumi, Hesam, Mehrpour, Omid, Farrokhfall, Khadijeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791802/
https://www.ncbi.nlm.nih.gov/pubmed/33413696
http://dx.doi.org/10.1186/s40360-020-00469-4
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author Nakhaee, Samaneh
Dastjerdi, Mohammad
Roumi, Hesam
Mehrpour, Omid
Farrokhfall, Khadijeh
author_facet Nakhaee, Samaneh
Dastjerdi, Mohammad
Roumi, Hesam
Mehrpour, Omid
Farrokhfall, Khadijeh
author_sort Nakhaee, Samaneh
collection PubMed
description BACKGROUND: Acetaminophen (APAP) induced hepatotoxicity is a clinically important problem. Up to now, interventive therapy with n-acetylcysteine (NAC) has been considered as a gold-standard treatment for APAP overdose. However, no study has focused on the efficacy of these drugs’ concurrent administration on probable enhancing therapeutic outcomes. Thus, this study was aimed to investigate the analgesic effect of co-administration of NAC and acetaminophen in male rats. The NAC-APAP drug formulation may demonstrate the stranger antinociceptive effect. METHODS: Forty-eight male Sprague-Dawley rats (12–14 weeks) randomly divided into six equal groups; control, APAP (received 300 mg/kg APAP), NAC (received 600 mg/kg NAC) and APAP+ NAC groups that received simultaneously 300 mg/kg APAP with 200–600 mg/kg NAC (AN200, AN400, AN600). All administrations were done orally for once. The antinociceptive effect was recorded by measurement of latency period on a hot plate in 30, 60, and 90 min after administrations. RESULTS: The results showed that NAC’s concurrent administration with APAP, dose-dependently increased APAP analgesic effects (p< 0.0001). Moreover, NAC treatment exhibited an antinociceptive effect in 60 and 90 min, per se. The treatments had no adverse effect on liver enzymes and oxidative stress. CONCLUSION: Co-administration of NAC with APAP can improve the antinociceptive effect of APAP. It is suggested that this compound can enhance analgesic effects of APAP and eventually lead to a reduction in acetaminophen dose. Further studies are needed to evaluate the molecular mechanism of this hyper analgesic effect.
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spelling pubmed-77918022021-01-11 N-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test Nakhaee, Samaneh Dastjerdi, Mohammad Roumi, Hesam Mehrpour, Omid Farrokhfall, Khadijeh BMC Pharmacol Toxicol Research Article BACKGROUND: Acetaminophen (APAP) induced hepatotoxicity is a clinically important problem. Up to now, interventive therapy with n-acetylcysteine (NAC) has been considered as a gold-standard treatment for APAP overdose. However, no study has focused on the efficacy of these drugs’ concurrent administration on probable enhancing therapeutic outcomes. Thus, this study was aimed to investigate the analgesic effect of co-administration of NAC and acetaminophen in male rats. The NAC-APAP drug formulation may demonstrate the stranger antinociceptive effect. METHODS: Forty-eight male Sprague-Dawley rats (12–14 weeks) randomly divided into six equal groups; control, APAP (received 300 mg/kg APAP), NAC (received 600 mg/kg NAC) and APAP+ NAC groups that received simultaneously 300 mg/kg APAP with 200–600 mg/kg NAC (AN200, AN400, AN600). All administrations were done orally for once. The antinociceptive effect was recorded by measurement of latency period on a hot plate in 30, 60, and 90 min after administrations. RESULTS: The results showed that NAC’s concurrent administration with APAP, dose-dependently increased APAP analgesic effects (p< 0.0001). Moreover, NAC treatment exhibited an antinociceptive effect in 60 and 90 min, per se. The treatments had no adverse effect on liver enzymes and oxidative stress. CONCLUSION: Co-administration of NAC with APAP can improve the antinociceptive effect of APAP. It is suggested that this compound can enhance analgesic effects of APAP and eventually lead to a reduction in acetaminophen dose. Further studies are needed to evaluate the molecular mechanism of this hyper analgesic effect. BioMed Central 2021-01-07 /pmc/articles/PMC7791802/ /pubmed/33413696 http://dx.doi.org/10.1186/s40360-020-00469-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Nakhaee, Samaneh
Dastjerdi, Mohammad
Roumi, Hesam
Mehrpour, Omid
Farrokhfall, Khadijeh
N-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test
title N-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test
title_full N-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test
title_fullStr N-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test
title_full_unstemmed N-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test
title_short N-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test
title_sort n-acetylcysteine dose-dependently improves the analgesic effect of acetaminophen on the rat hot plate test
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791802/
https://www.ncbi.nlm.nih.gov/pubmed/33413696
http://dx.doi.org/10.1186/s40360-020-00469-4
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