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Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells

As populations age across the world, osteoporosis and osteoporosis-related fractures are becoming the most prevalent degenerative bone diseases. More than 75 million patients suffer from osteoporosis in the USA, the EU and Japan. Furthermore, it is anticipated that the number of patients affected by...

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Autores principales: Bicer, Mesude, Cottrell, Graeme S., Widera, Darius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791873/
https://www.ncbi.nlm.nih.gov/pubmed/33413646
http://dx.doi.org/10.1186/s13287-020-02094-8
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author Bicer, Mesude
Cottrell, Graeme S.
Widera, Darius
author_facet Bicer, Mesude
Cottrell, Graeme S.
Widera, Darius
author_sort Bicer, Mesude
collection PubMed
description As populations age across the world, osteoporosis and osteoporosis-related fractures are becoming the most prevalent degenerative bone diseases. More than 75 million patients suffer from osteoporosis in the USA, the EU and Japan. Furthermore, it is anticipated that the number of patients affected by osteoporosis will increase by a third by 2050. Although conventional therapies including bisphosphonates, calcitonin and oestrogen-like drugs can be used to treat degenerative diseases of the bone, they are often associated with serious side effects including the development of oesophageal cancer, ocular inflammation, severe musculoskeletal pain and osteonecrosis of the jaw. The use of autologous mesenchymal stromal cells/mesenchymal stem cells (MSCs) is a possible alternative therapeutic approach to tackle osteoporosis while overcoming the limitations of traditional treatment options. However, osteoporosis can cause a decrease in the numbers of MSCs, induce their senescence and lower their osteogenic differentiation potential. Three-dimensional (3D) cell culture is an emerging technology that allows a more physiological expansion and differentiation of stem cells compared to cultivation on conventional flat systems. This review will discuss current understanding of the effects of different 3D cell culture systems on proliferation, viability and osteogenic differentiation, as well as on the immunomodulatory and anti-inflammatory potential of MSCs.
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spelling pubmed-77918732021-01-11 Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells Bicer, Mesude Cottrell, Graeme S. Widera, Darius Stem Cell Res Ther Review As populations age across the world, osteoporosis and osteoporosis-related fractures are becoming the most prevalent degenerative bone diseases. More than 75 million patients suffer from osteoporosis in the USA, the EU and Japan. Furthermore, it is anticipated that the number of patients affected by osteoporosis will increase by a third by 2050. Although conventional therapies including bisphosphonates, calcitonin and oestrogen-like drugs can be used to treat degenerative diseases of the bone, they are often associated with serious side effects including the development of oesophageal cancer, ocular inflammation, severe musculoskeletal pain and osteonecrosis of the jaw. The use of autologous mesenchymal stromal cells/mesenchymal stem cells (MSCs) is a possible alternative therapeutic approach to tackle osteoporosis while overcoming the limitations of traditional treatment options. However, osteoporosis can cause a decrease in the numbers of MSCs, induce their senescence and lower their osteogenic differentiation potential. Three-dimensional (3D) cell culture is an emerging technology that allows a more physiological expansion and differentiation of stem cells compared to cultivation on conventional flat systems. This review will discuss current understanding of the effects of different 3D cell culture systems on proliferation, viability and osteogenic differentiation, as well as on the immunomodulatory and anti-inflammatory potential of MSCs. BioMed Central 2021-01-07 /pmc/articles/PMC7791873/ /pubmed/33413646 http://dx.doi.org/10.1186/s13287-020-02094-8 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Review
Bicer, Mesude
Cottrell, Graeme S.
Widera, Darius
Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells
title Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells
title_full Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells
title_fullStr Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells
title_full_unstemmed Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells
title_short Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells
title_sort impact of 3d cell culture on bone regeneration potential of mesenchymal stromal cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791873/
https://www.ncbi.nlm.nih.gov/pubmed/33413646
http://dx.doi.org/10.1186/s13287-020-02094-8
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