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Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells

Nonalcoholic fatty liver disease (NAFLD) is a major cause of morbidity and mortality worldwide. Additional therapies using functional foods and dietary supplements have been investigated and used in clinical practice, showing them to be beneficial. Honeybee pollen from Chile has shown a large concen...

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Autores principales: Oyarzún, Juan Esteban, Andia, Marcelo E., Uribe, Sergio, Núñez Pizarro, Paula, Núñez, Gabriel, Montenegro, Gloria, Bridi, Raquel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792600/
https://www.ncbi.nlm.nih.gov/pubmed/33374984
http://dx.doi.org/10.3390/molecules26010006
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author Oyarzún, Juan Esteban
Andia, Marcelo E.
Uribe, Sergio
Núñez Pizarro, Paula
Núñez, Gabriel
Montenegro, Gloria
Bridi, Raquel
author_facet Oyarzún, Juan Esteban
Andia, Marcelo E.
Uribe, Sergio
Núñez Pizarro, Paula
Núñez, Gabriel
Montenegro, Gloria
Bridi, Raquel
author_sort Oyarzún, Juan Esteban
collection PubMed
description Nonalcoholic fatty liver disease (NAFLD) is a major cause of morbidity and mortality worldwide. Additional therapies using functional foods and dietary supplements have been investigated and used in clinical practice, showing them to be beneficial. Honeybee pollen from Chile has shown a large concentration of phenolic compounds and high antioxidant activity. In this work, we characterized twenty-eight bee pollen extracts from the central zone of Chile according to botanical origin, phenolic profile, quercetin concentration, and antioxidant activity (FRAP and ORAC-FL). Our results show a statistically significant positive correlation between total phenolic content and antioxidant capacity. Selected samples were evaluated on the ability to reverse the steatosis in an in vitro cell model using Hepa1-6 cells. The pollen extracts protected Hepa1-6 cells against oxidative damage triggered by 2,2′-azo-bis(2-amidinopropane) dihydrochloride (AAPH)derived free radicals. This effect can be credited to the ability of the phenolic compounds present in the extract to protect the liver cells from chemical-induced injury, which might be correlated to their free radical scavenging potential. Additionally, bee pollen extracts reduce lipid accumulation in a cellular model of steatosis. In summary, our results support the antioxidant, hepatoprotective, and anti-steatosis effect of bee pollen in an in vitro model.
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spelling pubmed-77926002021-01-09 Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells Oyarzún, Juan Esteban Andia, Marcelo E. Uribe, Sergio Núñez Pizarro, Paula Núñez, Gabriel Montenegro, Gloria Bridi, Raquel Molecules Article Nonalcoholic fatty liver disease (NAFLD) is a major cause of morbidity and mortality worldwide. Additional therapies using functional foods and dietary supplements have been investigated and used in clinical practice, showing them to be beneficial. Honeybee pollen from Chile has shown a large concentration of phenolic compounds and high antioxidant activity. In this work, we characterized twenty-eight bee pollen extracts from the central zone of Chile according to botanical origin, phenolic profile, quercetin concentration, and antioxidant activity (FRAP and ORAC-FL). Our results show a statistically significant positive correlation between total phenolic content and antioxidant capacity. Selected samples were evaluated on the ability to reverse the steatosis in an in vitro cell model using Hepa1-6 cells. The pollen extracts protected Hepa1-6 cells against oxidative damage triggered by 2,2′-azo-bis(2-amidinopropane) dihydrochloride (AAPH)derived free radicals. This effect can be credited to the ability of the phenolic compounds present in the extract to protect the liver cells from chemical-induced injury, which might be correlated to their free radical scavenging potential. Additionally, bee pollen extracts reduce lipid accumulation in a cellular model of steatosis. In summary, our results support the antioxidant, hepatoprotective, and anti-steatosis effect of bee pollen in an in vitro model. MDPI 2020-12-22 /pmc/articles/PMC7792600/ /pubmed/33374984 http://dx.doi.org/10.3390/molecules26010006 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oyarzún, Juan Esteban
Andia, Marcelo E.
Uribe, Sergio
Núñez Pizarro, Paula
Núñez, Gabriel
Montenegro, Gloria
Bridi, Raquel
Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells
title Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells
title_full Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells
title_fullStr Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells
title_full_unstemmed Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells
title_short Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells
title_sort honeybee pollen extracts reduce oxidative stress and steatosis in hepatic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792600/
https://www.ncbi.nlm.nih.gov/pubmed/33374984
http://dx.doi.org/10.3390/molecules26010006
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