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bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage

Natural Antisense Transcripts (NATs) are long non-coding RNAs (lncRNAs) that overlap coding genes in the opposite strand. NATs roles have been related to gene regulation through different mechanisms, including post-transcriptional RNA processing. With the aim to identify NATs with potential regulato...

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Autores principales: Pérez-Lluch, Sílvia, Klein, Cecilia C., Breschi, Alessandra, Ruiz-Romero, Marina, Abad, Amaya, Palumbo, Emilio, Bekish, Lyazzat, Arnan, Carme, Guigó, Roderic
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793246/
https://www.ncbi.nlm.nih.gov/pubmed/33370262
http://dx.doi.org/10.1371/journal.pgen.1009245
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author Pérez-Lluch, Sílvia
Klein, Cecilia C.
Breschi, Alessandra
Ruiz-Romero, Marina
Abad, Amaya
Palumbo, Emilio
Bekish, Lyazzat
Arnan, Carme
Guigó, Roderic
author_facet Pérez-Lluch, Sílvia
Klein, Cecilia C.
Breschi, Alessandra
Ruiz-Romero, Marina
Abad, Amaya
Palumbo, Emilio
Bekish, Lyazzat
Arnan, Carme
Guigó, Roderic
author_sort Pérez-Lluch, Sílvia
collection PubMed
description Natural Antisense Transcripts (NATs) are long non-coding RNAs (lncRNAs) that overlap coding genes in the opposite strand. NATs roles have been related to gene regulation through different mechanisms, including post-transcriptional RNA processing. With the aim to identify NATs with potential regulatory function during fly development, we generated RNA-Seq data in Drosophila developing tissues and found bsAS, one of the most highly expressed lncRNAs in the fly wing. bsAS is antisense to bs/DSRF, a gene involved in wing development and neural processes. bsAS plays a crucial role in the tissue specific regulation of the expression of the bs/DSRF isoforms. This regulation is essential for the correct determination of cell fate during Drosophila development, as bsAS knockouts show highly aberrant phenotypes. Regulation of bs isoform usage by bsAS is mediated by specific physical interactions between the promoters of these two genes, which suggests a regulatory mechanism involving the collision of RNA polymerases transcribing in opposite directions. Evolutionary analysis suggests that bsAS NAT emerged simultaneously to the long-short isoform structure of bs, preceding the emergence of wings in insects.
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spelling pubmed-77932462021-01-27 bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage Pérez-Lluch, Sílvia Klein, Cecilia C. Breschi, Alessandra Ruiz-Romero, Marina Abad, Amaya Palumbo, Emilio Bekish, Lyazzat Arnan, Carme Guigó, Roderic PLoS Genet Research Article Natural Antisense Transcripts (NATs) are long non-coding RNAs (lncRNAs) that overlap coding genes in the opposite strand. NATs roles have been related to gene regulation through different mechanisms, including post-transcriptional RNA processing. With the aim to identify NATs with potential regulatory function during fly development, we generated RNA-Seq data in Drosophila developing tissues and found bsAS, one of the most highly expressed lncRNAs in the fly wing. bsAS is antisense to bs/DSRF, a gene involved in wing development and neural processes. bsAS plays a crucial role in the tissue specific regulation of the expression of the bs/DSRF isoforms. This regulation is essential for the correct determination of cell fate during Drosophila development, as bsAS knockouts show highly aberrant phenotypes. Regulation of bs isoform usage by bsAS is mediated by specific physical interactions between the promoters of these two genes, which suggests a regulatory mechanism involving the collision of RNA polymerases transcribing in opposite directions. Evolutionary analysis suggests that bsAS NAT emerged simultaneously to the long-short isoform structure of bs, preceding the emergence of wings in insects. Public Library of Science 2020-12-28 /pmc/articles/PMC7793246/ /pubmed/33370262 http://dx.doi.org/10.1371/journal.pgen.1009245 Text en © 2020 Pérez-Lluch et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pérez-Lluch, Sílvia
Klein, Cecilia C.
Breschi, Alessandra
Ruiz-Romero, Marina
Abad, Amaya
Palumbo, Emilio
Bekish, Lyazzat
Arnan, Carme
Guigó, Roderic
bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage
title bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage
title_full bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage
title_fullStr bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage
title_full_unstemmed bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage
title_short bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage
title_sort bsas, an antisense long non-coding rna, essential for correct wing development through regulation of blistered/dsrf isoform usage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793246/
https://www.ncbi.nlm.nih.gov/pubmed/33370262
http://dx.doi.org/10.1371/journal.pgen.1009245
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