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Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy

Leptospirosis is a re-emerging and globally spread zoonosis caused by pathogenic genomospecies of Leptospira. Wild boar (Sus scrofa) are an important Leptospira host and are increasing in population all over Europe. The aim of this investigation was to evaluate Leptospira spp. infection in the repro...

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Autores principales: Cilia, Giovanni, Bertelloni, Fabrizio, Piredda, Ivana, Ponti, Maria Nicoletta, Turchi, Barbara, Cantile, Carlo, Parisi, Francesca, Pinzauti, Paolo, Armani, Andrea, Palmas, Bruna, Noworol, Malgorzata, Cerri, Domenico, Fratini, Filippo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793250/
https://www.ncbi.nlm.nih.gov/pubmed/33370309
http://dx.doi.org/10.1371/journal.pntd.0008982
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author Cilia, Giovanni
Bertelloni, Fabrizio
Piredda, Ivana
Ponti, Maria Nicoletta
Turchi, Barbara
Cantile, Carlo
Parisi, Francesca
Pinzauti, Paolo
Armani, Andrea
Palmas, Bruna
Noworol, Malgorzata
Cerri, Domenico
Fratini, Filippo
author_facet Cilia, Giovanni
Bertelloni, Fabrizio
Piredda, Ivana
Ponti, Maria Nicoletta
Turchi, Barbara
Cantile, Carlo
Parisi, Francesca
Pinzauti, Paolo
Armani, Andrea
Palmas, Bruna
Noworol, Malgorzata
Cerri, Domenico
Fratini, Filippo
author_sort Cilia, Giovanni
collection PubMed
description Leptospirosis is a re-emerging and globally spread zoonosis caused by pathogenic genomospecies of Leptospira. Wild boar (Sus scrofa) are an important Leptospira host and are increasing in population all over Europe. The aim of this investigation was to evaluate Leptospira spp. infection in the reproductive systems of wild boar hunted in two Italian regions: Tuscany and Sardinia. From 231 animals, reproductive system tissue samples (testicles, epididymides, uteri) as well as placentas and fetuses were collected. Bacteriological examination and Real-Time PCR were performed to detect pathogenic Leptospira (lipL32 gene). Leptospires were isolated from the testicles and epididymides of one adult and two subadult wild boar. Four isolates from the two subadult males were identified as Leptospira interrogans serogroup Australis by MLST, whereas Leptospira kirschneri serogroup Grippotyphosa was identified from the adult testicles and epididymis. Using Real-Time PCR, 70 samples were positive: 22 testicles (23.16%) and 22 epididymides (23.16%), 10 uteri (7.35%), 3 placentas (6.66%), and 13 fetuses (28.88%). Amplification of the rrs2 gene identified L. interrogans and L. kirschneri species. The results from this investigation confirmed that wild boar represent a potential source of pathogenic Leptospira spp. Isolation of Leptospira serogroups Australis and Grippotyphosa from the male reproductive system and the positive Real-Time PCR results from both male and female samples could suggest venereal transmission, as already demonstrated in pigs. Furthermore, placentas and fetuses were positive for the lipL32 target, and this finding may be related to a possible vertical transmission of pathogenic Leptospira.
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spelling pubmed-77932502021-01-27 Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy Cilia, Giovanni Bertelloni, Fabrizio Piredda, Ivana Ponti, Maria Nicoletta Turchi, Barbara Cantile, Carlo Parisi, Francesca Pinzauti, Paolo Armani, Andrea Palmas, Bruna Noworol, Malgorzata Cerri, Domenico Fratini, Filippo PLoS Negl Trop Dis Research Article Leptospirosis is a re-emerging and globally spread zoonosis caused by pathogenic genomospecies of Leptospira. Wild boar (Sus scrofa) are an important Leptospira host and are increasing in population all over Europe. The aim of this investigation was to evaluate Leptospira spp. infection in the reproductive systems of wild boar hunted in two Italian regions: Tuscany and Sardinia. From 231 animals, reproductive system tissue samples (testicles, epididymides, uteri) as well as placentas and fetuses were collected. Bacteriological examination and Real-Time PCR were performed to detect pathogenic Leptospira (lipL32 gene). Leptospires were isolated from the testicles and epididymides of one adult and two subadult wild boar. Four isolates from the two subadult males were identified as Leptospira interrogans serogroup Australis by MLST, whereas Leptospira kirschneri serogroup Grippotyphosa was identified from the adult testicles and epididymis. Using Real-Time PCR, 70 samples were positive: 22 testicles (23.16%) and 22 epididymides (23.16%), 10 uteri (7.35%), 3 placentas (6.66%), and 13 fetuses (28.88%). Amplification of the rrs2 gene identified L. interrogans and L. kirschneri species. The results from this investigation confirmed that wild boar represent a potential source of pathogenic Leptospira spp. Isolation of Leptospira serogroups Australis and Grippotyphosa from the male reproductive system and the positive Real-Time PCR results from both male and female samples could suggest venereal transmission, as already demonstrated in pigs. Furthermore, placentas and fetuses were positive for the lipL32 target, and this finding may be related to a possible vertical transmission of pathogenic Leptospira. Public Library of Science 2020-12-28 /pmc/articles/PMC7793250/ /pubmed/33370309 http://dx.doi.org/10.1371/journal.pntd.0008982 Text en © 2020 Cilia et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cilia, Giovanni
Bertelloni, Fabrizio
Piredda, Ivana
Ponti, Maria Nicoletta
Turchi, Barbara
Cantile, Carlo
Parisi, Francesca
Pinzauti, Paolo
Armani, Andrea
Palmas, Bruna
Noworol, Malgorzata
Cerri, Domenico
Fratini, Filippo
Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy
title Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy
title_full Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy
title_fullStr Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy
title_full_unstemmed Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy
title_short Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy
title_sort presence of pathogenic leptospira spp. in the reproductive system and fetuses of wild boars (sus scrofa) in italy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793250/
https://www.ncbi.nlm.nih.gov/pubmed/33370309
http://dx.doi.org/10.1371/journal.pntd.0008982
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