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Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics

Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretor...

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Detalles Bibliográficos
Autores principales: Cho, Pyo Yun, Lee, Ji-Yun, Kim, Tae Im, Song, Jin-Ho, Hong, Sung-Jong, Yoo, Won Gi, Tsuboi, Takafumi, Ha, Kwon-Soo, Jung, Jae-Wan, Takeo, Satoru, Han, Eun-Taek, Sripa, Banchob, Hong, Sung-Tae, Chai, Jong-Yil, Nam, Ho-Woo, Pak, Jhang Ho, Kim, Tong-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793300/
https://www.ncbi.nlm.nih.gov/pubmed/33370333
http://dx.doi.org/10.1371/journal.pntd.0008998
Descripción
Sumario:Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C. sinensis-infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini-infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.