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Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs

Morphogenesis and differentiation of organs is required for subsequent functional maturation. The morphological features of Peyer's patches vary among species. In pigs, they develop extensively in the ileum as ileal Peyer's patches (IPPs). However, the role of IPPs in the porcine immune sy...

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Autores principales: Furukawa, Mutsumi, Ito, Shun, Suzuki, Shunichi, Fuchimoto, Daiichiro, Onishi, Akira, Niimi, Kanae, Usami, Katsuki, Wu, Guoyao, Bazer, Fuller W., Ogasawara, Kouetsu, Watanabe, Kouichi, Aso, Hisashi, Nochi, Tomonori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793923/
https://www.ncbi.nlm.nih.gov/pubmed/33424851
http://dx.doi.org/10.3389/fimmu.2020.604674
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author Furukawa, Mutsumi
Ito, Shun
Suzuki, Shunichi
Fuchimoto, Daiichiro
Onishi, Akira
Niimi, Kanae
Usami, Katsuki
Wu, Guoyao
Bazer, Fuller W.
Ogasawara, Kouetsu
Watanabe, Kouichi
Aso, Hisashi
Nochi, Tomonori
author_facet Furukawa, Mutsumi
Ito, Shun
Suzuki, Shunichi
Fuchimoto, Daiichiro
Onishi, Akira
Niimi, Kanae
Usami, Katsuki
Wu, Guoyao
Bazer, Fuller W.
Ogasawara, Kouetsu
Watanabe, Kouichi
Aso, Hisashi
Nochi, Tomonori
author_sort Furukawa, Mutsumi
collection PubMed
description Morphogenesis and differentiation of organs is required for subsequent functional maturation. The morphological features of Peyer's patches vary among species. In pigs, they develop extensively in the ileum as ileal Peyer's patches (IPPs). However, the role of IPPs in the porcine immune system remains to be elucidated because of a lack of complete understanding of IPP organogenesis. Results of the present study revealed that development of porcine IPPs is initiated prenatally between embryonic days 76 and 91. The process of IPP organogenesis is concomitant with increased transcriptional patterns of CXCL13 and CCL19. IPPs undergo further development postnatally by forming central, marginal, and subepithelial zones. Importantly, a large number of proliferating B cells and apoptotic cells are found in porcine IPPs postnatally, but not prenatally. The expression level of IgM in proliferating B cells depends on the zone in which distinct B cells are separately localized after birth. Specifically, IgM(+) cells are predominantly found in the central zone, whereas IgM(-/low) cells are abundant in the marginal zone. Importantly, the cellular feature of IPPs differs from that of mesenteric lymph nodes (MLNs) where such distinct zones are not formed both prenatally and postnatally. Our findings suggest that IPPs (not MLNs) in postnatal pigs are involved in complementing functions of the primary lymphoid tissue that promotes the differentiation and maturation of B cells.
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spelling pubmed-77939232021-01-09 Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs Furukawa, Mutsumi Ito, Shun Suzuki, Shunichi Fuchimoto, Daiichiro Onishi, Akira Niimi, Kanae Usami, Katsuki Wu, Guoyao Bazer, Fuller W. Ogasawara, Kouetsu Watanabe, Kouichi Aso, Hisashi Nochi, Tomonori Front Immunol Immunology Morphogenesis and differentiation of organs is required for subsequent functional maturation. The morphological features of Peyer's patches vary among species. In pigs, they develop extensively in the ileum as ileal Peyer's patches (IPPs). However, the role of IPPs in the porcine immune system remains to be elucidated because of a lack of complete understanding of IPP organogenesis. Results of the present study revealed that development of porcine IPPs is initiated prenatally between embryonic days 76 and 91. The process of IPP organogenesis is concomitant with increased transcriptional patterns of CXCL13 and CCL19. IPPs undergo further development postnatally by forming central, marginal, and subepithelial zones. Importantly, a large number of proliferating B cells and apoptotic cells are found in porcine IPPs postnatally, but not prenatally. The expression level of IgM in proliferating B cells depends on the zone in which distinct B cells are separately localized after birth. Specifically, IgM(+) cells are predominantly found in the central zone, whereas IgM(-/low) cells are abundant in the marginal zone. Importantly, the cellular feature of IPPs differs from that of mesenteric lymph nodes (MLNs) where such distinct zones are not formed both prenatally and postnatally. Our findings suggest that IPPs (not MLNs) in postnatal pigs are involved in complementing functions of the primary lymphoid tissue that promotes the differentiation and maturation of B cells. Frontiers Media S.A. 2020-12-04 /pmc/articles/PMC7793923/ /pubmed/33424851 http://dx.doi.org/10.3389/fimmu.2020.604674 Text en Copyright © 2020 Furukawa, Ito, Suzuki, Fuchimoto, Onishi, Niimi, Usami, Wu, Bazer, Ogasawara, Watanabe, Aso and Nochi http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Furukawa, Mutsumi
Ito, Shun
Suzuki, Shunichi
Fuchimoto, Daiichiro
Onishi, Akira
Niimi, Kanae
Usami, Katsuki
Wu, Guoyao
Bazer, Fuller W.
Ogasawara, Kouetsu
Watanabe, Kouichi
Aso, Hisashi
Nochi, Tomonori
Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs
title Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs
title_full Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs
title_fullStr Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs
title_full_unstemmed Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs
title_short Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs
title_sort organogenesis of ileal peyer's patches is initiated prenatally and accelerated postnatally with comprehensive proliferation of b cells in pigs
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793923/
https://www.ncbi.nlm.nih.gov/pubmed/33424851
http://dx.doi.org/10.3389/fimmu.2020.604674
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