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Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?

Introducing desired mutations into the genome of model organisms is a priority for all research focusing on protein function and disease modeling. The need to create stable mutant lines has resulted in the rapid advancement of genetic techniques over the last few decades from chemical mutagenesis an...

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Autores principales: Prill, Kendal, Dawson, John F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793982/
https://www.ncbi.nlm.nih.gov/pubmed/33425990
http://dx.doi.org/10.3389/fmolb.2020.595474
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author Prill, Kendal
Dawson, John F.
author_facet Prill, Kendal
Dawson, John F.
author_sort Prill, Kendal
collection PubMed
description Introducing desired mutations into the genome of model organisms is a priority for all research focusing on protein function and disease modeling. The need to create stable mutant lines has resulted in the rapid advancement of genetic techniques over the last few decades from chemical mutagenesis and zinc finger nucleases to clustered regularly interspaced short palindromic repeats (CRISPR) and homology-directed repair (HDR). However, achieving consistently high success rates for direct mutagenesis in zebrafish remains one of the most sought-after techniques in the field. Several genes have been modified using HDR in zebrafish, but published success rates range widely, suggesting that an optimal protocol is required. In this review, we compare target genes, techniques, and protocols from 50 genes that were successfully modified in zebrafish using HDR to find the statistically best variables for efficient HDR rates.
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spelling pubmed-77939822021-01-09 Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry? Prill, Kendal Dawson, John F. Front Mol Biosci Molecular Biosciences Introducing desired mutations into the genome of model organisms is a priority for all research focusing on protein function and disease modeling. The need to create stable mutant lines has resulted in the rapid advancement of genetic techniques over the last few decades from chemical mutagenesis and zinc finger nucleases to clustered regularly interspaced short palindromic repeats (CRISPR) and homology-directed repair (HDR). However, achieving consistently high success rates for direct mutagenesis in zebrafish remains one of the most sought-after techniques in the field. Several genes have been modified using HDR in zebrafish, but published success rates range widely, suggesting that an optimal protocol is required. In this review, we compare target genes, techniques, and protocols from 50 genes that were successfully modified in zebrafish using HDR to find the statistically best variables for efficient HDR rates. Frontiers Media S.A. 2020-12-07 /pmc/articles/PMC7793982/ /pubmed/33425990 http://dx.doi.org/10.3389/fmolb.2020.595474 Text en Copyright © 2020 Prill and Dawson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Prill, Kendal
Dawson, John F.
Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?
title Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?
title_full Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?
title_fullStr Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?
title_full_unstemmed Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?
title_short Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?
title_sort homology-directed repair in zebrafish: witchcraft and wizardry?
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793982/
https://www.ncbi.nlm.nih.gov/pubmed/33425990
http://dx.doi.org/10.3389/fmolb.2020.595474
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