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Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?
Introducing desired mutations into the genome of model organisms is a priority for all research focusing on protein function and disease modeling. The need to create stable mutant lines has resulted in the rapid advancement of genetic techniques over the last few decades from chemical mutagenesis an...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793982/ https://www.ncbi.nlm.nih.gov/pubmed/33425990 http://dx.doi.org/10.3389/fmolb.2020.595474 |
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author | Prill, Kendal Dawson, John F. |
author_facet | Prill, Kendal Dawson, John F. |
author_sort | Prill, Kendal |
collection | PubMed |
description | Introducing desired mutations into the genome of model organisms is a priority for all research focusing on protein function and disease modeling. The need to create stable mutant lines has resulted in the rapid advancement of genetic techniques over the last few decades from chemical mutagenesis and zinc finger nucleases to clustered regularly interspaced short palindromic repeats (CRISPR) and homology-directed repair (HDR). However, achieving consistently high success rates for direct mutagenesis in zebrafish remains one of the most sought-after techniques in the field. Several genes have been modified using HDR in zebrafish, but published success rates range widely, suggesting that an optimal protocol is required. In this review, we compare target genes, techniques, and protocols from 50 genes that were successfully modified in zebrafish using HDR to find the statistically best variables for efficient HDR rates. |
format | Online Article Text |
id | pubmed-7793982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77939822021-01-09 Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry? Prill, Kendal Dawson, John F. Front Mol Biosci Molecular Biosciences Introducing desired mutations into the genome of model organisms is a priority for all research focusing on protein function and disease modeling. The need to create stable mutant lines has resulted in the rapid advancement of genetic techniques over the last few decades from chemical mutagenesis and zinc finger nucleases to clustered regularly interspaced short palindromic repeats (CRISPR) and homology-directed repair (HDR). However, achieving consistently high success rates for direct mutagenesis in zebrafish remains one of the most sought-after techniques in the field. Several genes have been modified using HDR in zebrafish, but published success rates range widely, suggesting that an optimal protocol is required. In this review, we compare target genes, techniques, and protocols from 50 genes that were successfully modified in zebrafish using HDR to find the statistically best variables for efficient HDR rates. Frontiers Media S.A. 2020-12-07 /pmc/articles/PMC7793982/ /pubmed/33425990 http://dx.doi.org/10.3389/fmolb.2020.595474 Text en Copyright © 2020 Prill and Dawson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Prill, Kendal Dawson, John F. Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry? |
title | Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry? |
title_full | Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry? |
title_fullStr | Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry? |
title_full_unstemmed | Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry? |
title_short | Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry? |
title_sort | homology-directed repair in zebrafish: witchcraft and wizardry? |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793982/ https://www.ncbi.nlm.nih.gov/pubmed/33425990 http://dx.doi.org/10.3389/fmolb.2020.595474 |
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