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Incidence and Diversity of Torix Rickettsia–Odonata Symbioses
Heritable microbes are an important component of invertebrate biology, acting both as beneficial symbionts and reproductive parasites. Whilst most previous research has focussed on the ‘Wolbachia pandemic’, recent work has emphasised the importance of other microbial symbionts. In this study, we pre...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794209/ https://www.ncbi.nlm.nih.gov/pubmed/32770272 http://dx.doi.org/10.1007/s00248-020-01568-9 |
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author | Thongprem, Panupong Davison, Helen R. Thompson, David J. Lorenzo-Carballa, M. Olalla Hurst, Gregory D. D. |
author_facet | Thongprem, Panupong Davison, Helen R. Thompson, David J. Lorenzo-Carballa, M. Olalla Hurst, Gregory D. D. |
author_sort | Thongprem, Panupong |
collection | PubMed |
description | Heritable microbes are an important component of invertebrate biology, acting both as beneficial symbionts and reproductive parasites. Whilst most previous research has focussed on the ‘Wolbachia pandemic’, recent work has emphasised the importance of other microbial symbionts. In this study, we present a survey of odonates (dragonflies and damselflies) for torix group Rickettsia, following previous research indicating that this clade can be common in other aquatic insect groups. PCR assays were used to screen a broad range of odonates from two continents and revealed 8 of 76 species tested were infected with Rickettsia. We then conducted further deeper screening of UK representatives of the Coenagrionidae damselfly family, revealing 6 of 8 UK coenagrionid species to be positive for torix Rickettsia. Analysis of Rickettsia gene sequences supported multiple establishments of symbiosis in the group. Some strains were shared between UK coenagrionid species that shared mtDNA barcodes, indicating a likely route for mitochondrial introgression between sister species. There was also evidence of coinfecting Rickettsia strains in two species. FISH analysis indicated Rickettsia were observed in the ovarioles, consistent with heritable symbiosis. We conclude that torix Rickettsia represent an important associate of odonates, being found in a broad range of species from both Europe and South America. There is evidence that coinfection can occur, vertical transmission is likely, and that symbiont movement following hybridisation may underpin the lack of ‘barcoding gap’ between well-established species pairs in the genus. Future work should establish the biological significance of the symbioses observed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00248-020-01568-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7794209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-77942092021-01-19 Incidence and Diversity of Torix Rickettsia–Odonata Symbioses Thongprem, Panupong Davison, Helen R. Thompson, David J. Lorenzo-Carballa, M. Olalla Hurst, Gregory D. D. Microb Ecol Invertebrate Microbiology Heritable microbes are an important component of invertebrate biology, acting both as beneficial symbionts and reproductive parasites. Whilst most previous research has focussed on the ‘Wolbachia pandemic’, recent work has emphasised the importance of other microbial symbionts. In this study, we present a survey of odonates (dragonflies and damselflies) for torix group Rickettsia, following previous research indicating that this clade can be common in other aquatic insect groups. PCR assays were used to screen a broad range of odonates from two continents and revealed 8 of 76 species tested were infected with Rickettsia. We then conducted further deeper screening of UK representatives of the Coenagrionidae damselfly family, revealing 6 of 8 UK coenagrionid species to be positive for torix Rickettsia. Analysis of Rickettsia gene sequences supported multiple establishments of symbiosis in the group. Some strains were shared between UK coenagrionid species that shared mtDNA barcodes, indicating a likely route for mitochondrial introgression between sister species. There was also evidence of coinfecting Rickettsia strains in two species. FISH analysis indicated Rickettsia were observed in the ovarioles, consistent with heritable symbiosis. We conclude that torix Rickettsia represent an important associate of odonates, being found in a broad range of species from both Europe and South America. There is evidence that coinfection can occur, vertical transmission is likely, and that symbiont movement following hybridisation may underpin the lack of ‘barcoding gap’ between well-established species pairs in the genus. Future work should establish the biological significance of the symbioses observed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00248-020-01568-9) contains supplementary material, which is available to authorized users. Springer US 2020-08-07 2021 /pmc/articles/PMC7794209/ /pubmed/32770272 http://dx.doi.org/10.1007/s00248-020-01568-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Invertebrate Microbiology Thongprem, Panupong Davison, Helen R. Thompson, David J. Lorenzo-Carballa, M. Olalla Hurst, Gregory D. D. Incidence and Diversity of Torix Rickettsia–Odonata Symbioses |
title | Incidence and Diversity of Torix Rickettsia–Odonata Symbioses |
title_full | Incidence and Diversity of Torix Rickettsia–Odonata Symbioses |
title_fullStr | Incidence and Diversity of Torix Rickettsia–Odonata Symbioses |
title_full_unstemmed | Incidence and Diversity of Torix Rickettsia–Odonata Symbioses |
title_short | Incidence and Diversity of Torix Rickettsia–Odonata Symbioses |
title_sort | incidence and diversity of torix rickettsia–odonata symbioses |
topic | Invertebrate Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794209/ https://www.ncbi.nlm.nih.gov/pubmed/32770272 http://dx.doi.org/10.1007/s00248-020-01568-9 |
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