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The gravistimulation-induced very slow Ca(2+) increase in Arabidopsis seedlings requires MCA1, a Ca(2+)-permeable mechanosensitive channel
Gravity is a critical environmental factor affecting the morphology and function of plants on Earth. Gravistimulation triggered by changes in the gravity vector induces an increase in the cytoplasmic free calcium ion concentration ([Ca(2+)](c)) as an early process of gravity sensing; however, its ro...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794229/ https://www.ncbi.nlm.nih.gov/pubmed/33420331 http://dx.doi.org/10.1038/s41598-020-80733-z |
Sumario: | Gravity is a critical environmental factor affecting the morphology and function of plants on Earth. Gravistimulation triggered by changes in the gravity vector induces an increase in the cytoplasmic free calcium ion concentration ([Ca(2+)](c)) as an early process of gravity sensing; however, its role and molecular mechanism are still unclear. When seedlings of Arabidopsis thaliana expressing apoaequorin were rotated from the upright position to the upside-down position, a biphasic [Ca(2+)](c)-increase composed of a fast-transient [Ca(2+)](c)-increase followed by a slow [Ca(2+)](c)-increase was observed. We find here a novel type [Ca(2+)](c)-increase, designated a very slow [Ca(2+)](c)-increase that is observed when the seedlings were rotated back to the upright position from the upside-down position. The very slow [Ca(2+)](c)-increase was strongly attenuated in knockout seedlings defective in MCA1, a mechanosensitive Ca(2+)-permeable channel (MSCC), and was partially restored in MCA1-complemented seedlings. The mechanosensitive ion channel blocker, gadolinium, blocked the very slow [Ca(2+)](c)-increase. This is the first report suggesting the possible involvement of MCA1 in an early event related to gravity sensing in Arabidopsis seedlings. |
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