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The gravistimulation-induced very slow Ca(2+) increase in Arabidopsis seedlings requires MCA1, a Ca(2+)-permeable mechanosensitive channel

Gravity is a critical environmental factor affecting the morphology and function of plants on Earth. Gravistimulation triggered by changes in the gravity vector induces an increase in the cytoplasmic free calcium ion concentration ([Ca(2+)](c)) as an early process of gravity sensing; however, its ro...

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Detalles Bibliográficos
Autores principales: Nakano, Masataka, Furuichi, Takuya, Sokabe, Masahiro, Iida, Hidetoshi, Tatsumi, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794229/
https://www.ncbi.nlm.nih.gov/pubmed/33420331
http://dx.doi.org/10.1038/s41598-020-80733-z
Descripción
Sumario:Gravity is a critical environmental factor affecting the morphology and function of plants on Earth. Gravistimulation triggered by changes in the gravity vector induces an increase in the cytoplasmic free calcium ion concentration ([Ca(2+)](c)) as an early process of gravity sensing; however, its role and molecular mechanism are still unclear. When seedlings of Arabidopsis thaliana expressing apoaequorin were rotated from the upright position to the upside-down position, a biphasic [Ca(2+)](c)-increase composed of a fast-transient [Ca(2+)](c)-increase followed by a slow [Ca(2+)](c)-increase was observed. We find here a novel type [Ca(2+)](c)-increase, designated a very slow [Ca(2+)](c)-increase that is observed when the seedlings were rotated back to the upright position from the upside-down position. The very slow [Ca(2+)](c)-increase was strongly attenuated in knockout seedlings defective in MCA1, a mechanosensitive Ca(2+)-permeable channel (MSCC), and was partially restored in MCA1-complemented seedlings. The mechanosensitive ion channel blocker, gadolinium, blocked the very slow [Ca(2+)](c)-increase. This is the first report suggesting the possible involvement of MCA1 in an early event related to gravity sensing in Arabidopsis seedlings.