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Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma
The gut microbiota is reported to modulate the immune response in hepatocellular carcinoma (HCC). Here, we employ metagenomic and metabolomic studies to characterise gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD) related cirrhosis, with or without HCC, and evaluate its eff...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794332/ https://www.ncbi.nlm.nih.gov/pubmed/33420074 http://dx.doi.org/10.1038/s41467-020-20422-7 |
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author | Behary, Jason Amorim, Nadia Jiang, Xiao-Tao Raposo, Anita Gong, Lan McGovern, Emily Ibrahim, Ragy Chu, Francis Stephens, Carlie Jebeili, Hazem Fragomeli, Vincenzo Koay, Yen Chin Jackson, Miriam O’Sullivan, John Weltman, Martin McCaughan, Geoffrey El-Omar, Emad Zekry, Amany |
author_facet | Behary, Jason Amorim, Nadia Jiang, Xiao-Tao Raposo, Anita Gong, Lan McGovern, Emily Ibrahim, Ragy Chu, Francis Stephens, Carlie Jebeili, Hazem Fragomeli, Vincenzo Koay, Yen Chin Jackson, Miriam O’Sullivan, John Weltman, Martin McCaughan, Geoffrey El-Omar, Emad Zekry, Amany |
author_sort | Behary, Jason |
collection | PubMed |
description | The gut microbiota is reported to modulate the immune response in hepatocellular carcinoma (HCC). Here, we employ metagenomic and metabolomic studies to characterise gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD) related cirrhosis, with or without HCC, and evaluate its effect on the peripheral immune response in an ex vivo model. We find that dysbiosis characterises the microbiota of patients with NAFLD-cirrhosis, with compositional and functional shifts occurring with HCC development. Gene function of the microbiota in NAFLD-HCC supports short chain fatty acid production, and this is confirmed by metabolomic studies. Ex vivo studies show that bacterial extracts from the NAFLD-HCC microbiota, but not from the control groups, elicit a T cell immunosuppressive phenotype, characterised by expansion of regulatory T cells and attenuation of CD8 + T cells. Our study suggest that the gut microbiota in NAFLD-HCC is characterised by a distinctive microbiome/metabolomic profile, and can modulate the peripheral immune response. |
format | Online Article Text |
id | pubmed-7794332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77943322021-01-15 Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma Behary, Jason Amorim, Nadia Jiang, Xiao-Tao Raposo, Anita Gong, Lan McGovern, Emily Ibrahim, Ragy Chu, Francis Stephens, Carlie Jebeili, Hazem Fragomeli, Vincenzo Koay, Yen Chin Jackson, Miriam O’Sullivan, John Weltman, Martin McCaughan, Geoffrey El-Omar, Emad Zekry, Amany Nat Commun Article The gut microbiota is reported to modulate the immune response in hepatocellular carcinoma (HCC). Here, we employ metagenomic and metabolomic studies to characterise gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD) related cirrhosis, with or without HCC, and evaluate its effect on the peripheral immune response in an ex vivo model. We find that dysbiosis characterises the microbiota of patients with NAFLD-cirrhosis, with compositional and functional shifts occurring with HCC development. Gene function of the microbiota in NAFLD-HCC supports short chain fatty acid production, and this is confirmed by metabolomic studies. Ex vivo studies show that bacterial extracts from the NAFLD-HCC microbiota, but not from the control groups, elicit a T cell immunosuppressive phenotype, characterised by expansion of regulatory T cells and attenuation of CD8 + T cells. Our study suggest that the gut microbiota in NAFLD-HCC is characterised by a distinctive microbiome/metabolomic profile, and can modulate the peripheral immune response. Nature Publishing Group UK 2021-01-08 /pmc/articles/PMC7794332/ /pubmed/33420074 http://dx.doi.org/10.1038/s41467-020-20422-7 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Behary, Jason Amorim, Nadia Jiang, Xiao-Tao Raposo, Anita Gong, Lan McGovern, Emily Ibrahim, Ragy Chu, Francis Stephens, Carlie Jebeili, Hazem Fragomeli, Vincenzo Koay, Yen Chin Jackson, Miriam O’Sullivan, John Weltman, Martin McCaughan, Geoffrey El-Omar, Emad Zekry, Amany Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma |
title | Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma |
title_full | Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma |
title_fullStr | Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma |
title_full_unstemmed | Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma |
title_short | Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma |
title_sort | gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794332/ https://www.ncbi.nlm.nih.gov/pubmed/33420074 http://dx.doi.org/10.1038/s41467-020-20422-7 |
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