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Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon

Copper is both essential and toxic to living beings, which tightly controls its intracellular concentration. At the host–pathogen interface, copper is used by phagocytic cells to kill invading microorganisms. We investigated copper homeostasis in Bordetella pertussis, which lives in the human respir...

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Autores principales: Rivera-Millot, Alex, Slupek, Stéphanie, Chatagnon, Jonathan, Roy, Gauthier, Saliou, Jean-Michel, Billon, Gabriel, Alaimo, Véronique, Hot, David, Salomé-Desnoulez, Sophie, Locht, Camille, Antoine, Rudy, Jacob-Dubuisson, Françoise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794356/
https://www.ncbi.nlm.nih.gov/pubmed/33420409
http://dx.doi.org/10.1038/s42003-020-01580-2
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author Rivera-Millot, Alex
Slupek, Stéphanie
Chatagnon, Jonathan
Roy, Gauthier
Saliou, Jean-Michel
Billon, Gabriel
Alaimo, Véronique
Hot, David
Salomé-Desnoulez, Sophie
Locht, Camille
Antoine, Rudy
Jacob-Dubuisson, Françoise
author_facet Rivera-Millot, Alex
Slupek, Stéphanie
Chatagnon, Jonathan
Roy, Gauthier
Saliou, Jean-Michel
Billon, Gabriel
Alaimo, Véronique
Hot, David
Salomé-Desnoulez, Sophie
Locht, Camille
Antoine, Rudy
Jacob-Dubuisson, Françoise
author_sort Rivera-Millot, Alex
collection PubMed
description Copper is both essential and toxic to living beings, which tightly controls its intracellular concentration. At the host–pathogen interface, copper is used by phagocytic cells to kill invading microorganisms. We investigated copper homeostasis in Bordetella pertussis, which lives in the human respiratory mucosa and has no environmental reservoir. B. pertussis has considerably streamlined copper homeostasis mechanisms relative to other Gram-negative bacteria. Its single remaining defense line consists of a metallochaperone diverted for copper passivation, CopZ, and two peroxide detoxification enzymes, PrxGrx and GorB, which together fight stresses encountered in phagocytic cells. Those proteins are encoded by an original, composite operon assembled in an environmental ancestor, which is under sensitive control by copper. This system appears to contribute to persistent infection in the nasal cavity of B. pertussis-infected mice. Combining responses to co-occurring stresses in a tailored operon reveals a strategy adopted by a host-restricted pathogen to optimize survival at minimal energy expenditure.
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spelling pubmed-77943562021-01-15 Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon Rivera-Millot, Alex Slupek, Stéphanie Chatagnon, Jonathan Roy, Gauthier Saliou, Jean-Michel Billon, Gabriel Alaimo, Véronique Hot, David Salomé-Desnoulez, Sophie Locht, Camille Antoine, Rudy Jacob-Dubuisson, Françoise Commun Biol Article Copper is both essential and toxic to living beings, which tightly controls its intracellular concentration. At the host–pathogen interface, copper is used by phagocytic cells to kill invading microorganisms. We investigated copper homeostasis in Bordetella pertussis, which lives in the human respiratory mucosa and has no environmental reservoir. B. pertussis has considerably streamlined copper homeostasis mechanisms relative to other Gram-negative bacteria. Its single remaining defense line consists of a metallochaperone diverted for copper passivation, CopZ, and two peroxide detoxification enzymes, PrxGrx and GorB, which together fight stresses encountered in phagocytic cells. Those proteins are encoded by an original, composite operon assembled in an environmental ancestor, which is under sensitive control by copper. This system appears to contribute to persistent infection in the nasal cavity of B. pertussis-infected mice. Combining responses to co-occurring stresses in a tailored operon reveals a strategy adopted by a host-restricted pathogen to optimize survival at minimal energy expenditure. Nature Publishing Group UK 2021-01-08 /pmc/articles/PMC7794356/ /pubmed/33420409 http://dx.doi.org/10.1038/s42003-020-01580-2 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rivera-Millot, Alex
Slupek, Stéphanie
Chatagnon, Jonathan
Roy, Gauthier
Saliou, Jean-Michel
Billon, Gabriel
Alaimo, Véronique
Hot, David
Salomé-Desnoulez, Sophie
Locht, Camille
Antoine, Rudy
Jacob-Dubuisson, Françoise
Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon
title Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon
title_full Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon
title_fullStr Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon
title_full_unstemmed Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon
title_short Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon
title_sort streamlined copper defenses make bordetella pertussis reliant on custom-made operon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794356/
https://www.ncbi.nlm.nih.gov/pubmed/33420409
http://dx.doi.org/10.1038/s42003-020-01580-2
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