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Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes

Myelodysplastic syndromes (MDS) are characterized by ineffective hematopoiesis and the abnormal differentiation of hematopoietic stem cells. An increasing number of researches have demonstrated that microRNAs play crucial roles in the pathogenesis of myelodysplastic syndromes. Herein, we aimed to id...

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Autores principales: Wan, Chengyao, Wen, Jing, Liang, Xiaolin, Xie, Qiongni, Wu, Wenqi, Wu, Meiqing, Liu, Zhenfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794569/
https://www.ncbi.nlm.nih.gov/pubmed/33420276
http://dx.doi.org/10.1038/s41598-020-80571-z
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author Wan, Chengyao
Wen, Jing
Liang, Xiaolin
Xie, Qiongni
Wu, Wenqi
Wu, Meiqing
Liu, Zhenfang
author_facet Wan, Chengyao
Wen, Jing
Liang, Xiaolin
Xie, Qiongni
Wu, Wenqi
Wu, Meiqing
Liu, Zhenfang
author_sort Wan, Chengyao
collection PubMed
description Myelodysplastic syndromes (MDS) are characterized by ineffective hematopoiesis and the abnormal differentiation of hematopoietic stem cells. An increasing number of researches have demonstrated that microRNAs play crucial roles in the pathogenesis of myelodysplastic syndromes. Herein, we aimed to identify novel potential microRNAs bound up with the diagnosis and prognosis of MDS. MiRNA microarray analysis was used to screen deregulated microRNAs in the bone marrow of MDS patients. qRT-PCR was employed to confirm the microarray results. All members of miR-320 family (miR-320a, miR-320b, miR-320c, miR-320d, and miR-320e) were significantly increased in MDS patients compared to normal control. Although we found no correlation between miR-320 family and most clinical characteristics, high miR-320c and miR-320d expression seemed to be associated with high numbers of bone marrow (BM) blasts and worse karyotype. High expression of all the members of the miR-320 family seemed to be associated with a high prognostic score based on International Prognostic Scoring System (IPSS). The areas under the miR-320 family member ROC curves were 0.9037 (P < 0.0001), 0.7515 (P = 0.0002), 0.9647 (P < 0.0001), 0.8064 (P < 0.0001) and 0.9019 (P < 0.0001). Regarding Kaplan–Meier analysis, high miR-320c and miR-320d expression were related to shorter overall survival (OS). Moreover, multivariate analysis revealed the independent prognostic value of miR-320d for OS in MDS. The expression of miR-320 family members was up-regulated in MDS, and miR-320 family members could serve as candidate diagnostic biomarkers for MDS. High expression of miR-320d was an independent prognostic factor for OS in MDS.
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spelling pubmed-77945692021-01-12 Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes Wan, Chengyao Wen, Jing Liang, Xiaolin Xie, Qiongni Wu, Wenqi Wu, Meiqing Liu, Zhenfang Sci Rep Article Myelodysplastic syndromes (MDS) are characterized by ineffective hematopoiesis and the abnormal differentiation of hematopoietic stem cells. An increasing number of researches have demonstrated that microRNAs play crucial roles in the pathogenesis of myelodysplastic syndromes. Herein, we aimed to identify novel potential microRNAs bound up with the diagnosis and prognosis of MDS. MiRNA microarray analysis was used to screen deregulated microRNAs in the bone marrow of MDS patients. qRT-PCR was employed to confirm the microarray results. All members of miR-320 family (miR-320a, miR-320b, miR-320c, miR-320d, and miR-320e) were significantly increased in MDS patients compared to normal control. Although we found no correlation between miR-320 family and most clinical characteristics, high miR-320c and miR-320d expression seemed to be associated with high numbers of bone marrow (BM) blasts and worse karyotype. High expression of all the members of the miR-320 family seemed to be associated with a high prognostic score based on International Prognostic Scoring System (IPSS). The areas under the miR-320 family member ROC curves were 0.9037 (P < 0.0001), 0.7515 (P = 0.0002), 0.9647 (P < 0.0001), 0.8064 (P < 0.0001) and 0.9019 (P < 0.0001). Regarding Kaplan–Meier analysis, high miR-320c and miR-320d expression were related to shorter overall survival (OS). Moreover, multivariate analysis revealed the independent prognostic value of miR-320d for OS in MDS. The expression of miR-320 family members was up-regulated in MDS, and miR-320 family members could serve as candidate diagnostic biomarkers for MDS. High expression of miR-320d was an independent prognostic factor for OS in MDS. Nature Publishing Group UK 2021-01-08 /pmc/articles/PMC7794569/ /pubmed/33420276 http://dx.doi.org/10.1038/s41598-020-80571-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wan, Chengyao
Wen, Jing
Liang, Xiaolin
Xie, Qiongni
Wu, Wenqi
Wu, Meiqing
Liu, Zhenfang
Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes
title Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes
title_full Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes
title_fullStr Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes
title_full_unstemmed Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes
title_short Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes
title_sort identification of mir-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794569/
https://www.ncbi.nlm.nih.gov/pubmed/33420276
http://dx.doi.org/10.1038/s41598-020-80571-z
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