T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?

We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood–based assays performed comparably in patients with...

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Autores principales: Lauruschkat, Chris D, Page, Lukas, Etter, Sonja, Weis, Philipp, Gamon, Florian, Kraus, Sabrina, Einsele, Hermann, Wurster, Sebastian, Loeffler, Juergen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794650/
https://www.ncbi.nlm.nih.gov/pubmed/33447629
http://dx.doi.org/10.1093/ofid/ofaa547
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author Lauruschkat, Chris D
Page, Lukas
Etter, Sonja
Weis, Philipp
Gamon, Florian
Kraus, Sabrina
Einsele, Hermann
Wurster, Sebastian
Loeffler, Juergen
author_facet Lauruschkat, Chris D
Page, Lukas
Etter, Sonja
Weis, Philipp
Gamon, Florian
Kraus, Sabrina
Einsele, Hermann
Wurster, Sebastian
Loeffler, Juergen
author_sort Lauruschkat, Chris D
collection PubMed
description We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood–based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.
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spelling pubmed-77946502021-01-13 T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT? Lauruschkat, Chris D Page, Lukas Etter, Sonja Weis, Philipp Gamon, Florian Kraus, Sabrina Einsele, Hermann Wurster, Sebastian Loeffler, Juergen Open Forum Infect Dis Brief Reports We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood–based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology. Oxford University Press 2020-11-08 /pmc/articles/PMC7794650/ /pubmed/33447629 http://dx.doi.org/10.1093/ofid/ofaa547 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Brief Reports
Lauruschkat, Chris D
Page, Lukas
Etter, Sonja
Weis, Philipp
Gamon, Florian
Kraus, Sabrina
Einsele, Hermann
Wurster, Sebastian
Loeffler, Juergen
T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?
title T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?
title_full T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?
title_fullStr T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?
title_full_unstemmed T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?
title_short T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?
title_sort t-cell immune surveillance in allogenic stem cell transplant recipients: are whole blood–based assays ready to challenge elispot?
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794650/
https://www.ncbi.nlm.nih.gov/pubmed/33447629
http://dx.doi.org/10.1093/ofid/ofaa547
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