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Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media
BACKGROUND: The evolving antibiotic-resistant behavior of health care–associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more. FINDING...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794652/ https://www.ncbi.nlm.nih.gov/pubmed/33420779 http://dx.doi.org/10.1093/gigascience/giaa156 |
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author | Rajput, Akanksha Poudel, Saugat Tsunemoto, Hannah Meehan, Michael Szubin, Richard Olson, Connor A Seif, Yara Lamsa, Anne Dillon, Nicholas Vrbanac, Alison Sugie, Joseph Dahesh, Samira Monk, Jonathan M Dorrestein, Pieter C Knight, Rob Pogliano, Joe Nizet, Victor Feist, Adam M Palsson, Bernhard O |
author_facet | Rajput, Akanksha Poudel, Saugat Tsunemoto, Hannah Meehan, Michael Szubin, Richard Olson, Connor A Seif, Yara Lamsa, Anne Dillon, Nicholas Vrbanac, Alison Sugie, Joseph Dahesh, Samira Monk, Jonathan M Dorrestein, Pieter C Knight, Rob Pogliano, Joe Nizet, Victor Feist, Adam M Palsson, Bernhard O |
author_sort | Rajput, Akanksha |
collection | PubMed |
description | BACKGROUND: The evolving antibiotic-resistant behavior of health care–associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more. FINDINGS: The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments. CONCLUSIONS: The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments. |
format | Online Article Text |
id | pubmed-7794652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-77946522021-01-13 Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media Rajput, Akanksha Poudel, Saugat Tsunemoto, Hannah Meehan, Michael Szubin, Richard Olson, Connor A Seif, Yara Lamsa, Anne Dillon, Nicholas Vrbanac, Alison Sugie, Joseph Dahesh, Samira Monk, Jonathan M Dorrestein, Pieter C Knight, Rob Pogliano, Joe Nizet, Victor Feist, Adam M Palsson, Bernhard O Gigascience Data Note BACKGROUND: The evolving antibiotic-resistant behavior of health care–associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more. FINDINGS: The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments. CONCLUSIONS: The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments. Oxford University Press 2021-01-09 /pmc/articles/PMC7794652/ /pubmed/33420779 http://dx.doi.org/10.1093/gigascience/giaa156 Text en © The Author(s) 2021. Published by Oxford University Press GigaScience. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Data Note Rajput, Akanksha Poudel, Saugat Tsunemoto, Hannah Meehan, Michael Szubin, Richard Olson, Connor A Seif, Yara Lamsa, Anne Dillon, Nicholas Vrbanac, Alison Sugie, Joseph Dahesh, Samira Monk, Jonathan M Dorrestein, Pieter C Knight, Rob Pogliano, Joe Nizet, Victor Feist, Adam M Palsson, Bernhard O Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media |
title | Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media |
title_full | Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media |
title_fullStr | Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media |
title_full_unstemmed | Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media |
title_short | Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media |
title_sort | identifying the effect of vancomycin on health care–associated methicillin-resistant staphylococcus aureus strains using bacteriological and physiological media |
topic | Data Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794652/ https://www.ncbi.nlm.nih.gov/pubmed/33420779 http://dx.doi.org/10.1093/gigascience/giaa156 |
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