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Tranexamic Acid Promotes Murine Bone Marrow-Derived Osteoblast Proliferation and Inhibits Osteoclast Formation In Vitro

Despite modern surgical trauma care, bleeding contributes to one-third of trauma-related death. A significant improvement was obtained through the introduction of tranexamic acid (TXA), which today is widely used in emergency and elective orthopedic surgery to control bleeding. However, concerns rem...

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Autores principales: Baranowsky, Anke, Appelt, Jessika, Tseneva, Kristina, Jiang, Shan, Jahn, Denise, Tsitsilonis, Serafeim, Frosch, Karl-Heinz, Keller, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795046/
https://www.ncbi.nlm.nih.gov/pubmed/33466312
http://dx.doi.org/10.3390/ijms22010449
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author Baranowsky, Anke
Appelt, Jessika
Tseneva, Kristina
Jiang, Shan
Jahn, Denise
Tsitsilonis, Serafeim
Frosch, Karl-Heinz
Keller, Johannes
author_facet Baranowsky, Anke
Appelt, Jessika
Tseneva, Kristina
Jiang, Shan
Jahn, Denise
Tsitsilonis, Serafeim
Frosch, Karl-Heinz
Keller, Johannes
author_sort Baranowsky, Anke
collection PubMed
description Despite modern surgical trauma care, bleeding contributes to one-third of trauma-related death. A significant improvement was obtained through the introduction of tranexamic acid (TXA), which today is widely used in emergency and elective orthopedic surgery to control bleeding. However, concerns remain regarding potential adverse effects on bone turnover and regeneration. Therefore, we employed standardized cell culture systems including primary osteoblasts, osteoclasts, and macrophages to evaluate potential effects of TXA on murine bone cells. While osteoblasts derived from calvarial digestion were not affected, TXA increased cell proliferation and matrix mineralization in bone marrow-derived osteoblasts. Short-term TXA treatment (6 h) failed to alter the expression of osteoblast markers; however, long-term TXA stimulation (10 days) was associated with the increased expression of genes involved in osteoblast differentiation and extracellular matrix synthesis. Similarly, whereas short-term TXA treatment did not affect gene expression in terminally differentiated osteoclasts, long-term TXA stimulation resulted in the potent inhibition of osteoclastogenesis. Finally, in bone marrow-derived macrophages activated with LPS, simultaneous TXA treatment led to a reduced expression of inflammatory cytokines and chemokines. Collectively, our study demonstrates a differential action of TXA on bone cells including osteoanabolic, anti-resorptive, and anti-inflammatory effects in vitro which suggests novel treatment applications.
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spelling pubmed-77950462021-01-10 Tranexamic Acid Promotes Murine Bone Marrow-Derived Osteoblast Proliferation and Inhibits Osteoclast Formation In Vitro Baranowsky, Anke Appelt, Jessika Tseneva, Kristina Jiang, Shan Jahn, Denise Tsitsilonis, Serafeim Frosch, Karl-Heinz Keller, Johannes Int J Mol Sci Article Despite modern surgical trauma care, bleeding contributes to one-third of trauma-related death. A significant improvement was obtained through the introduction of tranexamic acid (TXA), which today is widely used in emergency and elective orthopedic surgery to control bleeding. However, concerns remain regarding potential adverse effects on bone turnover and regeneration. Therefore, we employed standardized cell culture systems including primary osteoblasts, osteoclasts, and macrophages to evaluate potential effects of TXA on murine bone cells. While osteoblasts derived from calvarial digestion were not affected, TXA increased cell proliferation and matrix mineralization in bone marrow-derived osteoblasts. Short-term TXA treatment (6 h) failed to alter the expression of osteoblast markers; however, long-term TXA stimulation (10 days) was associated with the increased expression of genes involved in osteoblast differentiation and extracellular matrix synthesis. Similarly, whereas short-term TXA treatment did not affect gene expression in terminally differentiated osteoclasts, long-term TXA stimulation resulted in the potent inhibition of osteoclastogenesis. Finally, in bone marrow-derived macrophages activated with LPS, simultaneous TXA treatment led to a reduced expression of inflammatory cytokines and chemokines. Collectively, our study demonstrates a differential action of TXA on bone cells including osteoanabolic, anti-resorptive, and anti-inflammatory effects in vitro which suggests novel treatment applications. MDPI 2021-01-05 /pmc/articles/PMC7795046/ /pubmed/33466312 http://dx.doi.org/10.3390/ijms22010449 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baranowsky, Anke
Appelt, Jessika
Tseneva, Kristina
Jiang, Shan
Jahn, Denise
Tsitsilonis, Serafeim
Frosch, Karl-Heinz
Keller, Johannes
Tranexamic Acid Promotes Murine Bone Marrow-Derived Osteoblast Proliferation and Inhibits Osteoclast Formation In Vitro
title Tranexamic Acid Promotes Murine Bone Marrow-Derived Osteoblast Proliferation and Inhibits Osteoclast Formation In Vitro
title_full Tranexamic Acid Promotes Murine Bone Marrow-Derived Osteoblast Proliferation and Inhibits Osteoclast Formation In Vitro
title_fullStr Tranexamic Acid Promotes Murine Bone Marrow-Derived Osteoblast Proliferation and Inhibits Osteoclast Formation In Vitro
title_full_unstemmed Tranexamic Acid Promotes Murine Bone Marrow-Derived Osteoblast Proliferation and Inhibits Osteoclast Formation In Vitro
title_short Tranexamic Acid Promotes Murine Bone Marrow-Derived Osteoblast Proliferation and Inhibits Osteoclast Formation In Vitro
title_sort tranexamic acid promotes murine bone marrow-derived osteoblast proliferation and inhibits osteoclast formation in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795046/
https://www.ncbi.nlm.nih.gov/pubmed/33466312
http://dx.doi.org/10.3390/ijms22010449
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