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Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet

Developmental exposure to toxicants and diet can interact with an individual’s genetics and produce long-lasting metabolic adaptations. The different isoforms of the apolipoprotein E (APOE) are an important source of variability in metabolic disorders and influence the response to the pesticide chlo...

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Autores principales: Guardia-Escote, Laia, Blanco, Jordi, Basaure, Pia, Biosca-Brull, Judit, Verkaik-Schakel, Rikst Nynke, Cabré, Maria, Peris-Sampedro, Fiona, Pérez-Fernández, Cristian, Sánchez-Santed, Fernando, Plösch, Torsten, Domingo, José L., Colomina, Maria Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795072/
https://www.ncbi.nlm.nih.gov/pubmed/33383760
http://dx.doi.org/10.3390/ijerph18010184
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author Guardia-Escote, Laia
Blanco, Jordi
Basaure, Pia
Biosca-Brull, Judit
Verkaik-Schakel, Rikst Nynke
Cabré, Maria
Peris-Sampedro, Fiona
Pérez-Fernández, Cristian
Sánchez-Santed, Fernando
Plösch, Torsten
Domingo, José L.
Colomina, Maria Teresa
author_facet Guardia-Escote, Laia
Blanco, Jordi
Basaure, Pia
Biosca-Brull, Judit
Verkaik-Schakel, Rikst Nynke
Cabré, Maria
Peris-Sampedro, Fiona
Pérez-Fernández, Cristian
Sánchez-Santed, Fernando
Plösch, Torsten
Domingo, José L.
Colomina, Maria Teresa
author_sort Guardia-Escote, Laia
collection PubMed
description Developmental exposure to toxicants and diet can interact with an individual’s genetics and produce long-lasting metabolic adaptations. The different isoforms of the apolipoprotein E (APOE) are an important source of variability in metabolic disorders and influence the response to the pesticide chlorpyrifos (CPF). We aimed to study the epigenetic regulation on feeding control genes and the influence of postnatal CPF exposure, APOE genotype, and sex, and how these modifications impact on the metabolic response to a high-fat diet (HFD). Both male and female apoE3- and apoE4-TR mice were exposed to CPF on postnatal days 10–15. The DNA methylation pattern of proopiomelanocortin, neuropeptide Y, leptin receptor, and insulin-like growth factor 2 was studied in the hypothalamus. At adulthood, the mice were given a HFD for eight weeks. The results highlight the importance of sex in the epigenetic regulation and the implication of CPF treatment and APOE genotype. The body weight progression exhibited sex-dimorphic differences, apoE4-TR males being the most susceptible to the effects induced by CPF and HFD. Overall, these results underscore the pivotal role of sex, APOE genotype, and developmental exposure to CPF on subsequent metabolic disturbances later in life and show that sex is a key variable in epigenetic regulation.
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spelling pubmed-77950722021-01-10 Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet Guardia-Escote, Laia Blanco, Jordi Basaure, Pia Biosca-Brull, Judit Verkaik-Schakel, Rikst Nynke Cabré, Maria Peris-Sampedro, Fiona Pérez-Fernández, Cristian Sánchez-Santed, Fernando Plösch, Torsten Domingo, José L. Colomina, Maria Teresa Int J Environ Res Public Health Article Developmental exposure to toxicants and diet can interact with an individual’s genetics and produce long-lasting metabolic adaptations. The different isoforms of the apolipoprotein E (APOE) are an important source of variability in metabolic disorders and influence the response to the pesticide chlorpyrifos (CPF). We aimed to study the epigenetic regulation on feeding control genes and the influence of postnatal CPF exposure, APOE genotype, and sex, and how these modifications impact on the metabolic response to a high-fat diet (HFD). Both male and female apoE3- and apoE4-TR mice were exposed to CPF on postnatal days 10–15. The DNA methylation pattern of proopiomelanocortin, neuropeptide Y, leptin receptor, and insulin-like growth factor 2 was studied in the hypothalamus. At adulthood, the mice were given a HFD for eight weeks. The results highlight the importance of sex in the epigenetic regulation and the implication of CPF treatment and APOE genotype. The body weight progression exhibited sex-dimorphic differences, apoE4-TR males being the most susceptible to the effects induced by CPF and HFD. Overall, these results underscore the pivotal role of sex, APOE genotype, and developmental exposure to CPF on subsequent metabolic disturbances later in life and show that sex is a key variable in epigenetic regulation. MDPI 2020-12-29 2021-01 /pmc/articles/PMC7795072/ /pubmed/33383760 http://dx.doi.org/10.3390/ijerph18010184 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guardia-Escote, Laia
Blanco, Jordi
Basaure, Pia
Biosca-Brull, Judit
Verkaik-Schakel, Rikst Nynke
Cabré, Maria
Peris-Sampedro, Fiona
Pérez-Fernández, Cristian
Sánchez-Santed, Fernando
Plösch, Torsten
Domingo, José L.
Colomina, Maria Teresa
Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet
title Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet
title_full Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet
title_fullStr Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet
title_full_unstemmed Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet
title_short Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet
title_sort sex and exposure to postnatal chlorpyrifos influence the epigenetics of feeding-related genes in a transgenic apoe mouse model: long-term implications on body weight after a high-fat diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795072/
https://www.ncbi.nlm.nih.gov/pubmed/33383760
http://dx.doi.org/10.3390/ijerph18010184
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