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Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator?

In this article, construction of amperometric sensor(s) based on screen-printed carbon electrodes covered by thin layers of two types of carbon nanomaterials serving as amplifiers, and containing [Cu(bipy)(2)Cl]Cl∙5H(2)O complex is reported. Their performance and biomimetic activity towards two sele...

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Autores principales: Sýs, Milan, Mukherjee, Atripan, Jashari, Granit, Adam, Vojtěch, Ashrafi, Amir M., Novák, Miroslav, Richtera, Lukáš
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795177/
https://www.ncbi.nlm.nih.gov/pubmed/33383885
http://dx.doi.org/10.3390/ma14010113
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author Sýs, Milan
Mukherjee, Atripan
Jashari, Granit
Adam, Vojtěch
Ashrafi, Amir M.
Novák, Miroslav
Richtera, Lukáš
author_facet Sýs, Milan
Mukherjee, Atripan
Jashari, Granit
Adam, Vojtěch
Ashrafi, Amir M.
Novák, Miroslav
Richtera, Lukáš
author_sort Sýs, Milan
collection PubMed
description In this article, construction of amperometric sensor(s) based on screen-printed carbon electrodes covered by thin layers of two types of carbon nanomaterials serving as amplifiers, and containing [Cu(bipy)(2)Cl]Cl∙5H(2)O complex is reported. Their performance and biomimetic activity towards two selected neurotransmitters (dopamine and serotonin) was studied mainly using flow injection analysis (FIA). The important parameters of FIA such as working potential, flow rate, and pH were optimized. The mechanism of the catalytic activity is explained and experimentally confirmed. It reveals that presence of hydrogen peroxide plays a crucial role which leads to answer the title question: can presented complex really be considered as a tyrosinase biomimetic catalyst or only as a redox mediator?
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spelling pubmed-77951772021-01-10 Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator? Sýs, Milan Mukherjee, Atripan Jashari, Granit Adam, Vojtěch Ashrafi, Amir M. Novák, Miroslav Richtera, Lukáš Materials (Basel) Article In this article, construction of amperometric sensor(s) based on screen-printed carbon electrodes covered by thin layers of two types of carbon nanomaterials serving as amplifiers, and containing [Cu(bipy)(2)Cl]Cl∙5H(2)O complex is reported. Their performance and biomimetic activity towards two selected neurotransmitters (dopamine and serotonin) was studied mainly using flow injection analysis (FIA). The important parameters of FIA such as working potential, flow rate, and pH were optimized. The mechanism of the catalytic activity is explained and experimentally confirmed. It reveals that presence of hydrogen peroxide plays a crucial role which leads to answer the title question: can presented complex really be considered as a tyrosinase biomimetic catalyst or only as a redox mediator? MDPI 2020-12-29 /pmc/articles/PMC7795177/ /pubmed/33383885 http://dx.doi.org/10.3390/ma14010113 Text en © 2020 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Sýs, Milan
Mukherjee, Atripan
Jashari, Granit
Adam, Vojtěch
Ashrafi, Amir M.
Novák, Miroslav
Richtera, Lukáš
Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator?
title Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator?
title_full Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator?
title_fullStr Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator?
title_full_unstemmed Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator?
title_short Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator?
title_sort bis(2,2′-bipyridil)copper(ii) chloride complex: tyrosinase biomimetic catalyst or redox mediator?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795177/
https://www.ncbi.nlm.nih.gov/pubmed/33383885
http://dx.doi.org/10.3390/ma14010113
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