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Effect of Jakyakgamcho-Tang Extracts on H(2)O(2)-Induced C2C12 Myoblasts
Oxidative stress is a major contributor to muscle aging and loss of muscle tissue. Jakyakgamcho-tang (JGT) has been used in traditional Eastern medicine to treat muscle pain. Here, we compared the total phenolic and flavonoid contents in 30% ethanol and water extracts of JGT and tested the preventiv...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795328/ https://www.ncbi.nlm.nih.gov/pubmed/33406609 http://dx.doi.org/10.3390/molecules26010215 |
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author | Kim, Young Sook Yuk, Heung Joo Kim, Dong Seon |
author_facet | Kim, Young Sook Yuk, Heung Joo Kim, Dong Seon |
author_sort | Kim, Young Sook |
collection | PubMed |
description | Oxidative stress is a major contributor to muscle aging and loss of muscle tissue. Jakyakgamcho-tang (JGT) has been used in traditional Eastern medicine to treat muscle pain. Here, we compared the total phenolic and flavonoid contents in 30% ethanol and water extracts of JGT and tested the preventive effects against oxidative stress (hydrogen peroxide)-induced cell death in murine C2C12 skeletal muscle cells. The total phenolic content and total flavonoid content in 30% ethanol extracts of JGT were higher than those of water extracts of JGT. Ethanol extracts of JGT (JGT-E) had stronger antioxidant activities of 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl-scavenging activity (DPPH) than water extracts of JGT (JGT-W). JGT-E contained 19–53% (1.8 to 4.9-fold) more active compounds (i.e., albiflorin, liquiritin, pentagalloylglucose, isoliquiritin apioside, isoliquiritin, liquiritigenin, and glycyrrhizin) than JGT-W. The ethanol extracts of JGT inhibited hydrogen peroxide-induced cell death and intracellular reactive oxygen species generation more effectively than the water extract of JGT in a dose-dependent manner. For the first time, these results suggest that ethanol extract of JGT is relatively more efficacious at protecting against oxidative stress-induced muscle cell death. |
format | Online Article Text |
id | pubmed-7795328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77953282021-01-10 Effect of Jakyakgamcho-Tang Extracts on H(2)O(2)-Induced C2C12 Myoblasts Kim, Young Sook Yuk, Heung Joo Kim, Dong Seon Molecules Article Oxidative stress is a major contributor to muscle aging and loss of muscle tissue. Jakyakgamcho-tang (JGT) has been used in traditional Eastern medicine to treat muscle pain. Here, we compared the total phenolic and flavonoid contents in 30% ethanol and water extracts of JGT and tested the preventive effects against oxidative stress (hydrogen peroxide)-induced cell death in murine C2C12 skeletal muscle cells. The total phenolic content and total flavonoid content in 30% ethanol extracts of JGT were higher than those of water extracts of JGT. Ethanol extracts of JGT (JGT-E) had stronger antioxidant activities of 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl-scavenging activity (DPPH) than water extracts of JGT (JGT-W). JGT-E contained 19–53% (1.8 to 4.9-fold) more active compounds (i.e., albiflorin, liquiritin, pentagalloylglucose, isoliquiritin apioside, isoliquiritin, liquiritigenin, and glycyrrhizin) than JGT-W. The ethanol extracts of JGT inhibited hydrogen peroxide-induced cell death and intracellular reactive oxygen species generation more effectively than the water extract of JGT in a dose-dependent manner. For the first time, these results suggest that ethanol extract of JGT is relatively more efficacious at protecting against oxidative stress-induced muscle cell death. MDPI 2021-01-04 /pmc/articles/PMC7795328/ /pubmed/33406609 http://dx.doi.org/10.3390/molecules26010215 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Young Sook Yuk, Heung Joo Kim, Dong Seon Effect of Jakyakgamcho-Tang Extracts on H(2)O(2)-Induced C2C12 Myoblasts |
title | Effect of Jakyakgamcho-Tang Extracts on H(2)O(2)-Induced C2C12 Myoblasts |
title_full | Effect of Jakyakgamcho-Tang Extracts on H(2)O(2)-Induced C2C12 Myoblasts |
title_fullStr | Effect of Jakyakgamcho-Tang Extracts on H(2)O(2)-Induced C2C12 Myoblasts |
title_full_unstemmed | Effect of Jakyakgamcho-Tang Extracts on H(2)O(2)-Induced C2C12 Myoblasts |
title_short | Effect of Jakyakgamcho-Tang Extracts on H(2)O(2)-Induced C2C12 Myoblasts |
title_sort | effect of jakyakgamcho-tang extracts on h(2)o(2)-induced c2c12 myoblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795328/ https://www.ncbi.nlm.nih.gov/pubmed/33406609 http://dx.doi.org/10.3390/molecules26010215 |
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