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Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice

Osteoporosis is a common disease caused by an imbalance of processes between bone resorption by osteoclasts and bone formation by osteoblasts in postmenopausal women. The roots of Gentiana lutea L. (GL) are reported to have beneficial effects on various human diseases related to liver functions and...

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Autores principales: Park, Eunkuk, Lee, Chang Gun, Lim, Eunguk, Hwang, Seokjin, Yun, Seung Hee, Kim, Jeonghyun, Jeong, Hyesoo, Yong, Yoonjoong, Yun, Seong-Hoon, Choi, Chun Whan, Jin, Hyun-Seok, Jeong, Seon-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795511/
https://www.ncbi.nlm.nih.gov/pubmed/33379387
http://dx.doi.org/10.3390/ijms22010233
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author Park, Eunkuk
Lee, Chang Gun
Lim, Eunguk
Hwang, Seokjin
Yun, Seung Hee
Kim, Jeonghyun
Jeong, Hyesoo
Yong, Yoonjoong
Yun, Seong-Hoon
Choi, Chun Whan
Jin, Hyun-Seok
Jeong, Seon-Yong
author_facet Park, Eunkuk
Lee, Chang Gun
Lim, Eunguk
Hwang, Seokjin
Yun, Seung Hee
Kim, Jeonghyun
Jeong, Hyesoo
Yong, Yoonjoong
Yun, Seong-Hoon
Choi, Chun Whan
Jin, Hyun-Seok
Jeong, Seon-Yong
author_sort Park, Eunkuk
collection PubMed
description Osteoporosis is a common disease caused by an imbalance of processes between bone resorption by osteoclasts and bone formation by osteoblasts in postmenopausal women. The roots of Gentiana lutea L. (GL) are reported to have beneficial effects on various human diseases related to liver functions and gastrointestinal motility, as well as on arthritis. Here, we fractionated and isolated bioactive constituent(s) responsible for anti-osteoporotic effects of GL root extract. A single phytochemical compound, loganic acid, was identified as a candidate osteoprotective agent. Its anti-osteoporotic effects were examined in vitro and in vivo. Treatment with loganic acid significantly increased osteoblastic differentiation in preosteoblast MC3T3-E1 cells by promoting alkaline phosphatase activity and increasing mRNA expression levels of bone metabolic markers such as Alpl, Bglap, and Sp7. However, loganic acid inhibited osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. For in vivo experiments, the effect of loganic acid on ovariectomized (OVX) mice was examined for 12 weeks. Loganic acid prevented OVX-induced bone mineral density loss and improved bone structural properties in osteoporotic model mice. These results suggest that loganic acid may be a potential therapeutic candidate for treatment of osteoporosis.
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spelling pubmed-77955112021-01-10 Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice Park, Eunkuk Lee, Chang Gun Lim, Eunguk Hwang, Seokjin Yun, Seung Hee Kim, Jeonghyun Jeong, Hyesoo Yong, Yoonjoong Yun, Seong-Hoon Choi, Chun Whan Jin, Hyun-Seok Jeong, Seon-Yong Int J Mol Sci Article Osteoporosis is a common disease caused by an imbalance of processes between bone resorption by osteoclasts and bone formation by osteoblasts in postmenopausal women. The roots of Gentiana lutea L. (GL) are reported to have beneficial effects on various human diseases related to liver functions and gastrointestinal motility, as well as on arthritis. Here, we fractionated and isolated bioactive constituent(s) responsible for anti-osteoporotic effects of GL root extract. A single phytochemical compound, loganic acid, was identified as a candidate osteoprotective agent. Its anti-osteoporotic effects were examined in vitro and in vivo. Treatment with loganic acid significantly increased osteoblastic differentiation in preosteoblast MC3T3-E1 cells by promoting alkaline phosphatase activity and increasing mRNA expression levels of bone metabolic markers such as Alpl, Bglap, and Sp7. However, loganic acid inhibited osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. For in vivo experiments, the effect of loganic acid on ovariectomized (OVX) mice was examined for 12 weeks. Loganic acid prevented OVX-induced bone mineral density loss and improved bone structural properties in osteoporotic model mice. These results suggest that loganic acid may be a potential therapeutic candidate for treatment of osteoporosis. MDPI 2020-12-28 /pmc/articles/PMC7795511/ /pubmed/33379387 http://dx.doi.org/10.3390/ijms22010233 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Eunkuk
Lee, Chang Gun
Lim, Eunguk
Hwang, Seokjin
Yun, Seung Hee
Kim, Jeonghyun
Jeong, Hyesoo
Yong, Yoonjoong
Yun, Seong-Hoon
Choi, Chun Whan
Jin, Hyun-Seok
Jeong, Seon-Yong
Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice
title Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice
title_full Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice
title_fullStr Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice
title_full_unstemmed Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice
title_short Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice
title_sort osteoprotective effects of loganic acid on osteoblastic and osteoclastic cells and osteoporosis-induced mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795511/
https://www.ncbi.nlm.nih.gov/pubmed/33379387
http://dx.doi.org/10.3390/ijms22010233
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