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Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars

Insect pests pose a serious threat to global food production. Pod borer (Helicoverpa armigera (Hübner)) is one of the most destructive pests of leguminous crops. The use of host resistance has been an effective, environmentally friendly and sustainable approach for controlling several agricultural p...

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Autores principales: Njaci, Isaac, Ngugi-Dawit, Abigail, Oduor, Richard O., Kago, Leah, Williams, Brett, Hoang, Linh Thi My, Mundree, Sagadevan G., Ghimire, Sita R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795875/
https://www.ncbi.nlm.nih.gov/pubmed/33396747
http://dx.doi.org/10.3390/ijms22010309
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author Njaci, Isaac
Ngugi-Dawit, Abigail
Oduor, Richard O.
Kago, Leah
Williams, Brett
Hoang, Linh Thi My
Mundree, Sagadevan G.
Ghimire, Sita R.
author_facet Njaci, Isaac
Ngugi-Dawit, Abigail
Oduor, Richard O.
Kago, Leah
Williams, Brett
Hoang, Linh Thi My
Mundree, Sagadevan G.
Ghimire, Sita R.
author_sort Njaci, Isaac
collection PubMed
description Insect pests pose a serious threat to global food production. Pod borer (Helicoverpa armigera (Hübner)) is one of the most destructive pests of leguminous crops. The use of host resistance has been an effective, environmentally friendly and sustainable approach for controlling several agricultural pests. The exploitation of natural variations in crop wild relatives could yield pest-resistant crop varieties. In this study, we used a high-throughput transcriptome profiling approach to investigate the defense mechanisms of susceptible cultivated and tolerant wild pigeonpea genotypes against H. armigera infestation. The wild genotype displayed elevated pest-induced gene expression, including the enhanced induction of phytohormone and calcium/calmodulin signaling, transcription factors, plant volatiles and secondary metabolite genes compared to the cultivated control. The biosynthetic and regulatory processes associated with flavonoids, terpenes and glucosinolate secondary metabolites showed higher accumulations in the wild genotype, suggesting the existence of distinct tolerance mechanisms. This study provides insights into the molecular mechanisms underlying insect resistance in the wild pigeonpea genotype. This information highlights the indispensable role of crop wild relatives as a source of crucial genetic resources that could be important in devising strategies for crop improvement with enhanced pest resistance.
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spelling pubmed-77958752021-01-10 Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars Njaci, Isaac Ngugi-Dawit, Abigail Oduor, Richard O. Kago, Leah Williams, Brett Hoang, Linh Thi My Mundree, Sagadevan G. Ghimire, Sita R. Int J Mol Sci Article Insect pests pose a serious threat to global food production. Pod borer (Helicoverpa armigera (Hübner)) is one of the most destructive pests of leguminous crops. The use of host resistance has been an effective, environmentally friendly and sustainable approach for controlling several agricultural pests. The exploitation of natural variations in crop wild relatives could yield pest-resistant crop varieties. In this study, we used a high-throughput transcriptome profiling approach to investigate the defense mechanisms of susceptible cultivated and tolerant wild pigeonpea genotypes against H. armigera infestation. The wild genotype displayed elevated pest-induced gene expression, including the enhanced induction of phytohormone and calcium/calmodulin signaling, transcription factors, plant volatiles and secondary metabolite genes compared to the cultivated control. The biosynthetic and regulatory processes associated with flavonoids, terpenes and glucosinolate secondary metabolites showed higher accumulations in the wild genotype, suggesting the existence of distinct tolerance mechanisms. This study provides insights into the molecular mechanisms underlying insect resistance in the wild pigeonpea genotype. This information highlights the indispensable role of crop wild relatives as a source of crucial genetic resources that could be important in devising strategies for crop improvement with enhanced pest resistance. MDPI 2020-12-30 /pmc/articles/PMC7795875/ /pubmed/33396747 http://dx.doi.org/10.3390/ijms22010309 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Njaci, Isaac
Ngugi-Dawit, Abigail
Oduor, Richard O.
Kago, Leah
Williams, Brett
Hoang, Linh Thi My
Mundree, Sagadevan G.
Ghimire, Sita R.
Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars
title Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars
title_full Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars
title_fullStr Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars
title_full_unstemmed Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars
title_short Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars
title_sort comparative analysis delineates the transcriptional resistance mechanisms for pod borer resistance in the pigeonpea wild relative cajanus scarabaeoides (l.) thouars
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795875/
https://www.ncbi.nlm.nih.gov/pubmed/33396747
http://dx.doi.org/10.3390/ijms22010309
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