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Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells
Ferulic Acid (FA) is a highly abundant phenolic phytochemical which is present in plant tissues. FA has biological effects on physiological and pathological processes due to its anti-apoptotic and anti-oxidative properties, however, the detailed mechanism(s) of function is poorly understood. We have...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795901/ https://www.ncbi.nlm.nih.gov/pubmed/33379243 http://dx.doi.org/10.3390/molecules26010090 |
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author | Nakayama, Hironao Nakahara, Masako Matsugi, Erina Soda, Midori Hattori, Tomoka Hara, Koki Usami, Ayuki Kusumoto, Chiaki Higashiyama, Shigeki Kitaichi, Kiyoyuki |
author_facet | Nakayama, Hironao Nakahara, Masako Matsugi, Erina Soda, Midori Hattori, Tomoka Hara, Koki Usami, Ayuki Kusumoto, Chiaki Higashiyama, Shigeki Kitaichi, Kiyoyuki |
author_sort | Nakayama, Hironao |
collection | PubMed |
description | Ferulic Acid (FA) is a highly abundant phenolic phytochemical which is present in plant tissues. FA has biological effects on physiological and pathological processes due to its anti-apoptotic and anti-oxidative properties, however, the detailed mechanism(s) of function is poorly understood. We have identified FA as a molecule that inhibits apoptosis induced by hydrogen peroxide (H(2)O(2)) or actinomycin D (ActD) in rat pheochromocytoma, PC12 cell. We also found that FA reduces H(2)O(2)-induced reactive oxygen species (ROS) production in PC12 cell, thereby acting as an anti-oxidant. Then, we analyzed FA-mediated signaling responses in rat pheochromocytoma, PC12 cells using antibody arrays for phosphokinase and apoptosis related proteins. This FA signaling pathway in PC12 cells includes inactivation of pro-apoptotic proteins, SMAC/Diablo and Bad. In addition, FA attenuates the cell injury by H(2)O(2) through the inhibition of phosphorylation of the extracellular signal-regulated kinase (ERK). Importantly, we find that FA restores expression levels of brain-derived neurotrophic factor (BDNF), a key neuroprotective effector, in H(2)O(2)-treated PC12 cells. As a possible mechanism, FA increases BDNF by regulating microRNA-10b expression following H(2)O(2) stimulation. Taken together, FA has broad biological effects as a neuroprotective modulator to regulate the expression of phosphokinases, apoptosis-related proteins and microRNAs against oxidative stress in PC12 cells. |
format | Online Article Text |
id | pubmed-7795901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77959012021-01-10 Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells Nakayama, Hironao Nakahara, Masako Matsugi, Erina Soda, Midori Hattori, Tomoka Hara, Koki Usami, Ayuki Kusumoto, Chiaki Higashiyama, Shigeki Kitaichi, Kiyoyuki Molecules Article Ferulic Acid (FA) is a highly abundant phenolic phytochemical which is present in plant tissues. FA has biological effects on physiological and pathological processes due to its anti-apoptotic and anti-oxidative properties, however, the detailed mechanism(s) of function is poorly understood. We have identified FA as a molecule that inhibits apoptosis induced by hydrogen peroxide (H(2)O(2)) or actinomycin D (ActD) in rat pheochromocytoma, PC12 cell. We also found that FA reduces H(2)O(2)-induced reactive oxygen species (ROS) production in PC12 cell, thereby acting as an anti-oxidant. Then, we analyzed FA-mediated signaling responses in rat pheochromocytoma, PC12 cells using antibody arrays for phosphokinase and apoptosis related proteins. This FA signaling pathway in PC12 cells includes inactivation of pro-apoptotic proteins, SMAC/Diablo and Bad. In addition, FA attenuates the cell injury by H(2)O(2) through the inhibition of phosphorylation of the extracellular signal-regulated kinase (ERK). Importantly, we find that FA restores expression levels of brain-derived neurotrophic factor (BDNF), a key neuroprotective effector, in H(2)O(2)-treated PC12 cells. As a possible mechanism, FA increases BDNF by regulating microRNA-10b expression following H(2)O(2) stimulation. Taken together, FA has broad biological effects as a neuroprotective modulator to regulate the expression of phosphokinases, apoptosis-related proteins and microRNAs against oxidative stress in PC12 cells. MDPI 2020-12-28 /pmc/articles/PMC7795901/ /pubmed/33379243 http://dx.doi.org/10.3390/molecules26010090 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nakayama, Hironao Nakahara, Masako Matsugi, Erina Soda, Midori Hattori, Tomoka Hara, Koki Usami, Ayuki Kusumoto, Chiaki Higashiyama, Shigeki Kitaichi, Kiyoyuki Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells |
title | Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells |
title_full | Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells |
title_fullStr | Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells |
title_full_unstemmed | Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells |
title_short | Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells |
title_sort | protective effect of ferulic acid against hydrogen peroxide induced apoptosis in pc12 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795901/ https://www.ncbi.nlm.nih.gov/pubmed/33379243 http://dx.doi.org/10.3390/molecules26010090 |
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