Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark

A new alkaloid, geissospermiculatine was characterized in Geissospermum reticulatum A. H. Gentry bark (Apocynaceae). Here, following a simplified isolation protocol, the structure of the alkaloid was elucidated through GC-MS, LC-MS/MS, 1D, and 2D NMR (COSY, ROESY, HSQC, HMBC, (1)H-(15)N HMBC). Cytot...

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Autores principales: Sajkowska-Kozielewicz, Joanna J., Kozielewicz, Paweł, Makarova, Katerina, Stocki, Marcin, Barnes, Nicholas M., Paradowska, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795935/
https://www.ncbi.nlm.nih.gov/pubmed/33396182
http://dx.doi.org/10.3390/molecules26010143
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author Sajkowska-Kozielewicz, Joanna J.
Kozielewicz, Paweł
Makarova, Katerina
Stocki, Marcin
Barnes, Nicholas M.
Paradowska, Katarzyna
author_facet Sajkowska-Kozielewicz, Joanna J.
Kozielewicz, Paweł
Makarova, Katerina
Stocki, Marcin
Barnes, Nicholas M.
Paradowska, Katarzyna
author_sort Sajkowska-Kozielewicz, Joanna J.
collection PubMed
description A new alkaloid, geissospermiculatine was characterized in Geissospermum reticulatum A. H. Gentry bark (Apocynaceae). Here, following a simplified isolation protocol, the structure of the alkaloid was elucidated through GC-MS, LC-MS/MS, 1D, and 2D NMR (COSY, ROESY, HSQC, HMBC, (1)H-(15)N HMBC). Cytotoxic properties were evaluated in vitro on malignant THP-1 cells, and the results demonstrated that the cytotoxicity of the alkaloid (30  μg/mL) was comparable with staurosporine (10  μM). Additionally, the toxicity was tested on zebrafish (Danio rerio) embryos in vivo by monitoring their development (0–72 h); toxicity was not evident at 30  μg/mL.
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spelling pubmed-77959352021-01-10 Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark Sajkowska-Kozielewicz, Joanna J. Kozielewicz, Paweł Makarova, Katerina Stocki, Marcin Barnes, Nicholas M. Paradowska, Katarzyna Molecules Communication A new alkaloid, geissospermiculatine was characterized in Geissospermum reticulatum A. H. Gentry bark (Apocynaceae). Here, following a simplified isolation protocol, the structure of the alkaloid was elucidated through GC-MS, LC-MS/MS, 1D, and 2D NMR (COSY, ROESY, HSQC, HMBC, (1)H-(15)N HMBC). Cytotoxic properties were evaluated in vitro on malignant THP-1 cells, and the results demonstrated that the cytotoxicity of the alkaloid (30  μg/mL) was comparable with staurosporine (10  μM). Additionally, the toxicity was tested on zebrafish (Danio rerio) embryos in vivo by monitoring their development (0–72 h); toxicity was not evident at 30  μg/mL. MDPI 2020-12-31 /pmc/articles/PMC7795935/ /pubmed/33396182 http://dx.doi.org/10.3390/molecules26010143 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Sajkowska-Kozielewicz, Joanna J.
Kozielewicz, Paweł
Makarova, Katerina
Stocki, Marcin
Barnes, Nicholas M.
Paradowska, Katarzyna
Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark
title Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark
title_full Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark
title_fullStr Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark
title_full_unstemmed Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark
title_short Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark
title_sort geissospermiculatine, a new alkaloid from geissospermum reticulatum bark
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795935/
https://www.ncbi.nlm.nih.gov/pubmed/33396182
http://dx.doi.org/10.3390/molecules26010143
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