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A Fish Galectin-8 Possesses Direct Bactericidal Activity

Galectins are a family of animal lectins with high affinity for β-galactosides. Galectins are able to bind to bacteria, and a few mammalian galectins are known to kill the bound bacteria. In fish, no galectins with direct bactericidal effect have been reported. In the present study, we identified an...

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Autores principales: Zhang, Tengfei, Jiang, Shuai, Sun, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796122/
https://www.ncbi.nlm.nih.gov/pubmed/33396490
http://dx.doi.org/10.3390/ijms22010376
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author Zhang, Tengfei
Jiang, Shuai
Sun, Li
author_facet Zhang, Tengfei
Jiang, Shuai
Sun, Li
author_sort Zhang, Tengfei
collection PubMed
description Galectins are a family of animal lectins with high affinity for β-galactosides. Galectins are able to bind to bacteria, and a few mammalian galectins are known to kill the bound bacteria. In fish, no galectins with direct bactericidal effect have been reported. In the present study, we identified and characterized a tandem repeat galectin-8 from tongue sole Cynoglossus semilaevis (designated CsGal-8). CsGal-8 possesses conserved carbohydrate recognition domains (CRDs), as well as the conserved HXNPR and WGXEE motifs that are critical for carbohydrate binding. CsGal-8 was constitutively expressed in nine tissues of tongue sole and up-regulated in kidney, spleen, and blood by bacterial challenge. When expressed in HeLa cells, CsGal-8 protein was detected both in the cytoplasm and in the micro-vesicles secreted from the cells. Recombinant CsGal-8 (rCsGal-8) bound to lactose and other carbohydrates in a dose dependent manner. rCsGal-8 bound to a wide range of gram-positive and gram-negative bacteria and was co-localized with the bound bacteria in animal cells. Lactose, fructose, galactose, and trehalose effectively blocked the interactions between rCsGal-8 and different bacteria. Furthermore, rCsGal-8 exerted potent bactericidal activity against some gram-negative bacterial pathogens by directly damaging the membrane and structure of the pathogens. Taken together, these results indicate that CsGal-8 likely plays an important role in the immune defense against some bacterial pathogens by direct bacterial interaction and killing.
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spelling pubmed-77961222021-01-10 A Fish Galectin-8 Possesses Direct Bactericidal Activity Zhang, Tengfei Jiang, Shuai Sun, Li Int J Mol Sci Article Galectins are a family of animal lectins with high affinity for β-galactosides. Galectins are able to bind to bacteria, and a few mammalian galectins are known to kill the bound bacteria. In fish, no galectins with direct bactericidal effect have been reported. In the present study, we identified and characterized a tandem repeat galectin-8 from tongue sole Cynoglossus semilaevis (designated CsGal-8). CsGal-8 possesses conserved carbohydrate recognition domains (CRDs), as well as the conserved HXNPR and WGXEE motifs that are critical for carbohydrate binding. CsGal-8 was constitutively expressed in nine tissues of tongue sole and up-regulated in kidney, spleen, and blood by bacterial challenge. When expressed in HeLa cells, CsGal-8 protein was detected both in the cytoplasm and in the micro-vesicles secreted from the cells. Recombinant CsGal-8 (rCsGal-8) bound to lactose and other carbohydrates in a dose dependent manner. rCsGal-8 bound to a wide range of gram-positive and gram-negative bacteria and was co-localized with the bound bacteria in animal cells. Lactose, fructose, galactose, and trehalose effectively blocked the interactions between rCsGal-8 and different bacteria. Furthermore, rCsGal-8 exerted potent bactericidal activity against some gram-negative bacterial pathogens by directly damaging the membrane and structure of the pathogens. Taken together, these results indicate that CsGal-8 likely plays an important role in the immune defense against some bacterial pathogens by direct bacterial interaction and killing. MDPI 2020-12-31 /pmc/articles/PMC7796122/ /pubmed/33396490 http://dx.doi.org/10.3390/ijms22010376 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Tengfei
Jiang, Shuai
Sun, Li
A Fish Galectin-8 Possesses Direct Bactericidal Activity
title A Fish Galectin-8 Possesses Direct Bactericidal Activity
title_full A Fish Galectin-8 Possesses Direct Bactericidal Activity
title_fullStr A Fish Galectin-8 Possesses Direct Bactericidal Activity
title_full_unstemmed A Fish Galectin-8 Possesses Direct Bactericidal Activity
title_short A Fish Galectin-8 Possesses Direct Bactericidal Activity
title_sort fish galectin-8 possesses direct bactericidal activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796122/
https://www.ncbi.nlm.nih.gov/pubmed/33396490
http://dx.doi.org/10.3390/ijms22010376
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