Cargando…

Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

Most studies about dry eye disease (DED) chose unilateral eye for investigation and drew conclusions based on monocular results, whereas most studies involving tear proteomics were based on the results of pooling tears from a group of DED patients. Patients with DED were consecutively enrolled for b...

Descripción completa

Detalles Bibliográficos
Autores principales: Kuo, Ming-Tse, Fang, Po-Chiung, Kuo, Shu-Fang, Chen, Alexander, Huang, Yu-Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796155/
https://www.ncbi.nlm.nih.gov/pubmed/33401599
http://dx.doi.org/10.3390/ijms22010422
_version_ 1783634615058038784
author Kuo, Ming-Tse
Fang, Po-Chiung
Kuo, Shu-Fang
Chen, Alexander
Huang, Yu-Ting
author_facet Kuo, Ming-Tse
Fang, Po-Chiung
Kuo, Shu-Fang
Chen, Alexander
Huang, Yu-Ting
author_sort Kuo, Ming-Tse
collection PubMed
description Most studies about dry eye disease (DED) chose unilateral eye for investigation and drew conclusions based on monocular results, whereas most studies involving tear proteomics were based on the results of pooling tears from a group of DED patients. Patients with DED were consecutively enrolled for binocular clinical tests, tear biochemical markers of DED, and tear proteome. We found that bilateral eyes of DED patients may have similar but different ocular surface performance and tear proteome. Most ocular surface homeostatic markers and tear biomarkers were not significantly different in the bilateral eyes of DED subjects, and most clinical parameters and tear biomarkers were correlated significantly between bilateral eyes. However, discrepant binocular presentation in the markers of ocular surface homeostasis and the associations with tear proteins suggested that one eye’s performance cannot represent that of the other eye or both eyes. Therefore, in studies for elucidating tear film homeostasis of DED, we may lose some important messages hidden in the fellow eye if we collected clinical and proteomic data only from a unilateral eye. For mechanistic studies, it is recommended that researchers collect tear samples from the eye with more severe DED under sensitive criteria for identifying the more severe eye and evaluating the tear biochemical and proteomic markers with binocular concordance drawn in prior binocular studies.
format Online
Article
Text
id pubmed-7796155
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77961552021-01-10 Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study? Kuo, Ming-Tse Fang, Po-Chiung Kuo, Shu-Fang Chen, Alexander Huang, Yu-Ting Int J Mol Sci Article Most studies about dry eye disease (DED) chose unilateral eye for investigation and drew conclusions based on monocular results, whereas most studies involving tear proteomics were based on the results of pooling tears from a group of DED patients. Patients with DED were consecutively enrolled for binocular clinical tests, tear biochemical markers of DED, and tear proteome. We found that bilateral eyes of DED patients may have similar but different ocular surface performance and tear proteome. Most ocular surface homeostatic markers and tear biomarkers were not significantly different in the bilateral eyes of DED subjects, and most clinical parameters and tear biomarkers were correlated significantly between bilateral eyes. However, discrepant binocular presentation in the markers of ocular surface homeostasis and the associations with tear proteins suggested that one eye’s performance cannot represent that of the other eye or both eyes. Therefore, in studies for elucidating tear film homeostasis of DED, we may lose some important messages hidden in the fellow eye if we collected clinical and proteomic data only from a unilateral eye. For mechanistic studies, it is recommended that researchers collect tear samples from the eye with more severe DED under sensitive criteria for identifying the more severe eye and evaluating the tear biochemical and proteomic markers with binocular concordance drawn in prior binocular studies. MDPI 2021-01-03 /pmc/articles/PMC7796155/ /pubmed/33401599 http://dx.doi.org/10.3390/ijms22010422 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuo, Ming-Tse
Fang, Po-Chiung
Kuo, Shu-Fang
Chen, Alexander
Huang, Yu-Ting
Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?
title Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?
title_full Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?
title_fullStr Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?
title_full_unstemmed Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?
title_short Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?
title_sort tear proteomics study of dry eye disease: which eye do you adopt as the representative eye for the study?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796155/
https://www.ncbi.nlm.nih.gov/pubmed/33401599
http://dx.doi.org/10.3390/ijms22010422
work_keys_str_mv AT kuomingtse tearproteomicsstudyofdryeyediseasewhicheyedoyouadoptastherepresentativeeyeforthestudy
AT fangpochiung tearproteomicsstudyofdryeyediseasewhicheyedoyouadoptastherepresentativeeyeforthestudy
AT kuoshufang tearproteomicsstudyofdryeyediseasewhicheyedoyouadoptastherepresentativeeyeforthestudy
AT chenalexander tearproteomicsstudyofdryeyediseasewhicheyedoyouadoptastherepresentativeeyeforthestudy
AT huangyuting tearproteomicsstudyofdryeyediseasewhicheyedoyouadoptastherepresentativeeyeforthestudy