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Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR

SIMPLE SUMMARY: Sensitive detection of minimal residual disease by RT-qPCR in patients with neuroblastoma is shown to be predictive of outcome, but has not yet been introduced into clinical practice. A panel of multiple mRNA markers increases the sensitivity of minimal residual disease detection, si...

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Autores principales: van Zogchel, Lieke M. J., Zappeij-Kannegieter, Lily, Javadi, Ahmad, Lugtigheid, Marjolein, Gelineau, Nina U., Lak, Nathalie S. M., Zwijnenburg, Danny A., Koster, Jan, Stutterheim, Janine, van der Schoot, C. Ellen, Tytgat, Godelieve A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796198/
https://www.ncbi.nlm.nih.gov/pubmed/33466359
http://dx.doi.org/10.3390/cancers13010150
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author van Zogchel, Lieke M. J.
Zappeij-Kannegieter, Lily
Javadi, Ahmad
Lugtigheid, Marjolein
Gelineau, Nina U.
Lak, Nathalie S. M.
Zwijnenburg, Danny A.
Koster, Jan
Stutterheim, Janine
van der Schoot, C. Ellen
Tytgat, Godelieve A. M.
author_facet van Zogchel, Lieke M. J.
Zappeij-Kannegieter, Lily
Javadi, Ahmad
Lugtigheid, Marjolein
Gelineau, Nina U.
Lak, Nathalie S. M.
Zwijnenburg, Danny A.
Koster, Jan
Stutterheim, Janine
van der Schoot, C. Ellen
Tytgat, Godelieve A. M.
author_sort van Zogchel, Lieke M. J.
collection PubMed
description SIMPLE SUMMARY: Sensitive detection of minimal residual disease by RT-qPCR in patients with neuroblastoma is shown to be predictive of outcome, but has not yet been introduced into clinical practice. A panel of multiple mRNA markers increases the sensitivity of minimal residual disease detection, since neuroblastoma tumors are heterogeneous tumors. Recent studies have identified two distinct phenotypes, an adrenergic and mesenchymal phenotype, that can be identified by using different mRNA markers. As generally only small volumes of bone marrow or blood are available in young neuroblastoma patients, we have developed a multiplex RT-qPCR to be able to test seven different mRNA markers, while we reduce the sample volume needed. Comparison between the multiplex RT-qPCR and RT-qPCR for the single markers showed a comparable sensitivity. This reduction in required sample volume, while saving time and resources, can assist in the introduction of minimal residual disease detection by RT-qPCR into clinical practice. ABSTRACT: mRNA RT-qPCR is shown to be a very sensitive technique to detect minimal residual disease (MRD) in patients with neuroblastoma. Multiple mRNA markers are known to detect heterogeneous neuroblastoma cells in bone marrow (BM) or blood from patients. However, the limited volumes of BM and blood available can hamper the detection of multiple markers. To make optimal use of these samples, we developed a multiplex RT-qPCR for the detection of MRD in neuroblastoma. GUSB and PHOX2B were tested as single markers. The adrenergic markers TH, GAP43, CHRNA3 and DBH and mesenchymal markers POSTN, PRRX1 and FMO3 were tested in multiplex. Using control blood and BM, we established new thresholds for positivity. Comparison of multiplex and singleplex RT-qPCR results from 21 blood and 24 BM samples from neuroblastoma patients demonstrated a comparable sensitivity. With this multiplex RT-qPCR, we are able to test seven different neuroblastoma mRNA markers, which overcomes tumor heterogeneity and improves sensitivity of MRD detection, even in those samples of low RNA quantity. With resources and time being saved, reduction in sample volume and consumables can assist in the introduction of MRD by RT-qPCR into clinical practice.
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spelling pubmed-77961982021-01-10 Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR van Zogchel, Lieke M. J. Zappeij-Kannegieter, Lily Javadi, Ahmad Lugtigheid, Marjolein Gelineau, Nina U. Lak, Nathalie S. M. Zwijnenburg, Danny A. Koster, Jan Stutterheim, Janine van der Schoot, C. Ellen Tytgat, Godelieve A. M. Cancers (Basel) Article SIMPLE SUMMARY: Sensitive detection of minimal residual disease by RT-qPCR in patients with neuroblastoma is shown to be predictive of outcome, but has not yet been introduced into clinical practice. A panel of multiple mRNA markers increases the sensitivity of minimal residual disease detection, since neuroblastoma tumors are heterogeneous tumors. Recent studies have identified two distinct phenotypes, an adrenergic and mesenchymal phenotype, that can be identified by using different mRNA markers. As generally only small volumes of bone marrow or blood are available in young neuroblastoma patients, we have developed a multiplex RT-qPCR to be able to test seven different mRNA markers, while we reduce the sample volume needed. Comparison between the multiplex RT-qPCR and RT-qPCR for the single markers showed a comparable sensitivity. This reduction in required sample volume, while saving time and resources, can assist in the introduction of minimal residual disease detection by RT-qPCR into clinical practice. ABSTRACT: mRNA RT-qPCR is shown to be a very sensitive technique to detect minimal residual disease (MRD) in patients with neuroblastoma. Multiple mRNA markers are known to detect heterogeneous neuroblastoma cells in bone marrow (BM) or blood from patients. However, the limited volumes of BM and blood available can hamper the detection of multiple markers. To make optimal use of these samples, we developed a multiplex RT-qPCR for the detection of MRD in neuroblastoma. GUSB and PHOX2B were tested as single markers. The adrenergic markers TH, GAP43, CHRNA3 and DBH and mesenchymal markers POSTN, PRRX1 and FMO3 were tested in multiplex. Using control blood and BM, we established new thresholds for positivity. Comparison of multiplex and singleplex RT-qPCR results from 21 blood and 24 BM samples from neuroblastoma patients demonstrated a comparable sensitivity. With this multiplex RT-qPCR, we are able to test seven different neuroblastoma mRNA markers, which overcomes tumor heterogeneity and improves sensitivity of MRD detection, even in those samples of low RNA quantity. With resources and time being saved, reduction in sample volume and consumables can assist in the introduction of MRD by RT-qPCR into clinical practice. MDPI 2021-01-05 /pmc/articles/PMC7796198/ /pubmed/33466359 http://dx.doi.org/10.3390/cancers13010150 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
van Zogchel, Lieke M. J.
Zappeij-Kannegieter, Lily
Javadi, Ahmad
Lugtigheid, Marjolein
Gelineau, Nina U.
Lak, Nathalie S. M.
Zwijnenburg, Danny A.
Koster, Jan
Stutterheim, Janine
van der Schoot, C. Ellen
Tytgat, Godelieve A. M.
Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
title Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
title_full Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
title_fullStr Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
title_full_unstemmed Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
title_short Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
title_sort specific and sensitive detection of neuroblastoma mrna markers by multiplex rt-qpcr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796198/
https://www.ncbi.nlm.nih.gov/pubmed/33466359
http://dx.doi.org/10.3390/cancers13010150
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