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Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst

The immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 2...

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Autores principales: Ding, Yanshuai, Zhang, Hao, Wang, Xuelian, Zu, Hangtian, Wang, Cang, Dong, Dongxue, Lyu, Mingsheng, Wang, Shujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796272/
https://www.ncbi.nlm.nih.gov/pubmed/33396810
http://dx.doi.org/10.3390/ma14010130
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author Ding, Yanshuai
Zhang, Hao
Wang, Xuelian
Zu, Hangtian
Wang, Cang
Dong, Dongxue
Lyu, Mingsheng
Wang, Shujun
author_facet Ding, Yanshuai
Zhang, Hao
Wang, Xuelian
Zu, Hangtian
Wang, Cang
Dong, Dongxue
Lyu, Mingsheng
Wang, Shujun
author_sort Ding, Yanshuai
collection PubMed
description The immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 25 °C, 120 min, and pH 5, respectively. Dextranase could be loaded at 359.7 U/g. The immobilized dextranase was characterized by field emission gun-scanning electron microscope (FEG-SEM), X-ray diffraction (XRD), and Fourier-transformed infrared spectroscopy (FT-IR). The hydrolysis capacity of the immobilized enzyme was maintained at 71% at the 30th time of use. According to the constant temperature acceleration experiment, it was estimated that the immobilized dextranase could be stored for 99 days at 20 °C, indicating that the immobilized enzyme had good storage properties. Sodium chloride and sodium acetic did not desorb the immobilized dextranase. In contrast, dextranase was desorbed by sodium fluoride and sodium citrate. The hydrolysates were 79% oligosaccharides. The immobilized dextranase could significantly and thoroughly remove the dental plaque biofilm. Thus, immobilized dextranase has broad potential application in diverse fields in the future.
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spelling pubmed-77962722021-01-10 Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst Ding, Yanshuai Zhang, Hao Wang, Xuelian Zu, Hangtian Wang, Cang Dong, Dongxue Lyu, Mingsheng Wang, Shujun Materials (Basel) Article The immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 25 °C, 120 min, and pH 5, respectively. Dextranase could be loaded at 359.7 U/g. The immobilized dextranase was characterized by field emission gun-scanning electron microscope (FEG-SEM), X-ray diffraction (XRD), and Fourier-transformed infrared spectroscopy (FT-IR). The hydrolysis capacity of the immobilized enzyme was maintained at 71% at the 30th time of use. According to the constant temperature acceleration experiment, it was estimated that the immobilized dextranase could be stored for 99 days at 20 °C, indicating that the immobilized enzyme had good storage properties. Sodium chloride and sodium acetic did not desorb the immobilized dextranase. In contrast, dextranase was desorbed by sodium fluoride and sodium citrate. The hydrolysates were 79% oligosaccharides. The immobilized dextranase could significantly and thoroughly remove the dental plaque biofilm. Thus, immobilized dextranase has broad potential application in diverse fields in the future. MDPI 2020-12-30 /pmc/articles/PMC7796272/ /pubmed/33396810 http://dx.doi.org/10.3390/ma14010130 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ding, Yanshuai
Zhang, Hao
Wang, Xuelian
Zu, Hangtian
Wang, Cang
Dong, Dongxue
Lyu, Mingsheng
Wang, Shujun
Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst
title Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst
title_full Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst
title_fullStr Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst
title_full_unstemmed Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst
title_short Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst
title_sort immobilization of dextranase on nano-hydroxyapatite as a recyclable catalyst
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796272/
https://www.ncbi.nlm.nih.gov/pubmed/33396810
http://dx.doi.org/10.3390/ma14010130
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