Cargando…
Tumor-Targeting Peptides Search Strategy for the Delivery of Therapeutic and Diagnostic Molecules to Tumor Cells
Background: The combination of the unique properties of cancer cells makes it possible to find specific ligands that interact directly with the tumor, and to conduct targeted tumor therapy. Phage display is one of the most common methods for searching for specific ligands. Bacteriophages display pep...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796297/ https://www.ncbi.nlm.nih.gov/pubmed/33396774 http://dx.doi.org/10.3390/ijms22010314 |
_version_ | 1783634648897683456 |
---|---|
author | Dmitrieva, Maria D. Voitova, Anna A. Dymova, Maya A. Richter, Vladimir A. Kuligina, Elena V. |
author_facet | Dmitrieva, Maria D. Voitova, Anna A. Dymova, Maya A. Richter, Vladimir A. Kuligina, Elena V. |
author_sort | Dmitrieva, Maria D. |
collection | PubMed |
description | Background: The combination of the unique properties of cancer cells makes it possible to find specific ligands that interact directly with the tumor, and to conduct targeted tumor therapy. Phage display is one of the most common methods for searching for specific ligands. Bacteriophages display peptides, and the peptides themselves can be used as targeting molecules for the delivery of diagnostic and therapeutic agents. Phage display can be performed both in vitro and in vivo. Moreover, it is possible to carry out the phage display on cells pre-enriched for a certain tumor marker, for example, CD44 and CD133. Methods: For this work we used several methods, such as phage display, sequencing, cell sorting, immunocytochemistry, phage titration. Results: We performed phage display using different screening systems (in vitro and in vivo), different phage libraries (Ph.D-7, Ph.D-12, Ph.D-C7C) on CD44+/CD133+ and without enrichment U-87 MG cells. The binding efficiency of bacteriophages displayed tumor-targeting peptides on U-87 MG cells was compared in vitro. We also conducted a comparative analysis in vivo of the specificity of the accumulation of selected bacteriophages in the tumor and in the control organs (liver, brain, kidney and lungs). Conclusions: The screening in vivo of linear phage peptide libraries for glioblastoma was the most effective strategy for obtaining tumor-targeting peptides providing targeted delivery of diagnostic and therapeutic agents to glioblastoma. |
format | Online Article Text |
id | pubmed-7796297 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77962972021-01-10 Tumor-Targeting Peptides Search Strategy for the Delivery of Therapeutic and Diagnostic Molecules to Tumor Cells Dmitrieva, Maria D. Voitova, Anna A. Dymova, Maya A. Richter, Vladimir A. Kuligina, Elena V. Int J Mol Sci Article Background: The combination of the unique properties of cancer cells makes it possible to find specific ligands that interact directly with the tumor, and to conduct targeted tumor therapy. Phage display is one of the most common methods for searching for specific ligands. Bacteriophages display peptides, and the peptides themselves can be used as targeting molecules for the delivery of diagnostic and therapeutic agents. Phage display can be performed both in vitro and in vivo. Moreover, it is possible to carry out the phage display on cells pre-enriched for a certain tumor marker, for example, CD44 and CD133. Methods: For this work we used several methods, such as phage display, sequencing, cell sorting, immunocytochemistry, phage titration. Results: We performed phage display using different screening systems (in vitro and in vivo), different phage libraries (Ph.D-7, Ph.D-12, Ph.D-C7C) on CD44+/CD133+ and without enrichment U-87 MG cells. The binding efficiency of bacteriophages displayed tumor-targeting peptides on U-87 MG cells was compared in vitro. We also conducted a comparative analysis in vivo of the specificity of the accumulation of selected bacteriophages in the tumor and in the control organs (liver, brain, kidney and lungs). Conclusions: The screening in vivo of linear phage peptide libraries for glioblastoma was the most effective strategy for obtaining tumor-targeting peptides providing targeted delivery of diagnostic and therapeutic agents to glioblastoma. MDPI 2020-12-30 /pmc/articles/PMC7796297/ /pubmed/33396774 http://dx.doi.org/10.3390/ijms22010314 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dmitrieva, Maria D. Voitova, Anna A. Dymova, Maya A. Richter, Vladimir A. Kuligina, Elena V. Tumor-Targeting Peptides Search Strategy for the Delivery of Therapeutic and Diagnostic Molecules to Tumor Cells |
title | Tumor-Targeting Peptides Search Strategy for the Delivery of Therapeutic and Diagnostic Molecules to Tumor Cells |
title_full | Tumor-Targeting Peptides Search Strategy for the Delivery of Therapeutic and Diagnostic Molecules to Tumor Cells |
title_fullStr | Tumor-Targeting Peptides Search Strategy for the Delivery of Therapeutic and Diagnostic Molecules to Tumor Cells |
title_full_unstemmed | Tumor-Targeting Peptides Search Strategy for the Delivery of Therapeutic and Diagnostic Molecules to Tumor Cells |
title_short | Tumor-Targeting Peptides Search Strategy for the Delivery of Therapeutic and Diagnostic Molecules to Tumor Cells |
title_sort | tumor-targeting peptides search strategy for the delivery of therapeutic and diagnostic molecules to tumor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796297/ https://www.ncbi.nlm.nih.gov/pubmed/33396774 http://dx.doi.org/10.3390/ijms22010314 |
work_keys_str_mv | AT dmitrievamariad tumortargetingpeptidessearchstrategyforthedeliveryoftherapeuticanddiagnosticmoleculestotumorcells AT voitovaannaa tumortargetingpeptidessearchstrategyforthedeliveryoftherapeuticanddiagnosticmoleculestotumorcells AT dymovamayaa tumortargetingpeptidessearchstrategyforthedeliveryoftherapeuticanddiagnosticmoleculestotumorcells AT richtervladimira tumortargetingpeptidessearchstrategyforthedeliveryoftherapeuticanddiagnosticmoleculestotumorcells AT kuliginaelenav tumortargetingpeptidessearchstrategyforthedeliveryoftherapeuticanddiagnosticmoleculestotumorcells |