Cargando…

Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip

The heating of a biologic solution is a crucial part in an amplification process such as the catalytic detection of a biological target. However, in many situations, heating must be limited in microfluidic devices, as high temperatures can cause the denaturation of the chip components. Local heating...

Descripción completa

Detalles Bibliográficos
Autores principales: Horny, Marie-Charlotte, Dupuis, Vincent, Siaugue, Jean-Michel, Gamby, Jean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796339/
https://www.ncbi.nlm.nih.gov/pubmed/33383936
http://dx.doi.org/10.3390/s21010185
_version_ 1783634658871738368
author Horny, Marie-Charlotte
Dupuis, Vincent
Siaugue, Jean-Michel
Gamby, Jean
author_facet Horny, Marie-Charlotte
Dupuis, Vincent
Siaugue, Jean-Michel
Gamby, Jean
author_sort Horny, Marie-Charlotte
collection PubMed
description The heating of a biologic solution is a crucial part in an amplification process such as the catalytic detection of a biological target. However, in many situations, heating must be limited in microfluidic devices, as high temperatures can cause the denaturation of the chip components. Local heating through magnetic hyperthermia on magnetic nano-objects has opened the doors to numerous improvements, such as for oncology where a reduced heating allows the synergy of chemotherapy and thermotherapy. Here we report on the design and implementation of a lab on chip without global heating of samples. It takes advantage of the extreme efficiency of DNA-modified superparamagnetic core–shell nanoparticles to capture complementary sequences (microRNA-target), uses magnetic hyperthermia to locally release these targets, and detects them through electrochemical techniques using ultra-sensitive channel DNA-modified ultramicroelectrodes. The combination of magnetic hyperthermia and microfluidics coupled with on-chip electrochemistry opens the way to a drastic reduction in the time devoted to the steps of extraction, amplification and nucleic acids detection. The originality comes from the design and microfabrication of the microfluidic chip suitable to its insertion in the millimetric gap of toric inductance with a ferrite core.
format Online
Article
Text
id pubmed-7796339
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77963392021-01-10 Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip Horny, Marie-Charlotte Dupuis, Vincent Siaugue, Jean-Michel Gamby, Jean Sensors (Basel) Article The heating of a biologic solution is a crucial part in an amplification process such as the catalytic detection of a biological target. However, in many situations, heating must be limited in microfluidic devices, as high temperatures can cause the denaturation of the chip components. Local heating through magnetic hyperthermia on magnetic nano-objects has opened the doors to numerous improvements, such as for oncology where a reduced heating allows the synergy of chemotherapy and thermotherapy. Here we report on the design and implementation of a lab on chip without global heating of samples. It takes advantage of the extreme efficiency of DNA-modified superparamagnetic core–shell nanoparticles to capture complementary sequences (microRNA-target), uses magnetic hyperthermia to locally release these targets, and detects them through electrochemical techniques using ultra-sensitive channel DNA-modified ultramicroelectrodes. The combination of magnetic hyperthermia and microfluidics coupled with on-chip electrochemistry opens the way to a drastic reduction in the time devoted to the steps of extraction, amplification and nucleic acids detection. The originality comes from the design and microfabrication of the microfluidic chip suitable to its insertion in the millimetric gap of toric inductance with a ferrite core. MDPI 2020-12-29 /pmc/articles/PMC7796339/ /pubmed/33383936 http://dx.doi.org/10.3390/s21010185 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Horny, Marie-Charlotte
Dupuis, Vincent
Siaugue, Jean-Michel
Gamby, Jean
Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip
title Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip
title_full Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip
title_fullStr Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip
title_full_unstemmed Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip
title_short Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip
title_sort release and detection of microrna by combining magnetic hyperthermia and electrochemistry modules on a microfluidic chip
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796339/
https://www.ncbi.nlm.nih.gov/pubmed/33383936
http://dx.doi.org/10.3390/s21010185
work_keys_str_mv AT hornymariecharlotte releaseanddetectionofmicrornabycombiningmagnetichyperthermiaandelectrochemistrymodulesonamicrofluidicchip
AT dupuisvincent releaseanddetectionofmicrornabycombiningmagnetichyperthermiaandelectrochemistrymodulesonamicrofluidicchip
AT siauguejeanmichel releaseanddetectionofmicrornabycombiningmagnetichyperthermiaandelectrochemistrymodulesonamicrofluidicchip
AT gambyjean releaseanddetectionofmicrornabycombiningmagnetichyperthermiaandelectrochemistrymodulesonamicrofluidicchip