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Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil

The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to t...

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Autores principales: Silva, Gedean Galdino da Cruz, Campana, Eloiza Helena, Vasconcelos, Priscylla Carvalho, da Silva, Núbia Michelle Vieira, Santos Filho, Lauro, Leite, Elma Lima, Givisiez, Patrícia Emília Naves, Gebreyes, Wondwossen Abebe, de Oliveira, Celso José Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796378/
https://www.ncbi.nlm.nih.gov/pubmed/33375538
http://dx.doi.org/10.3390/ijerph18010095
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author Silva, Gedean Galdino da Cruz
Campana, Eloiza Helena
Vasconcelos, Priscylla Carvalho
da Silva, Núbia Michelle Vieira
Santos Filho, Lauro
Leite, Elma Lima
Givisiez, Patrícia Emília Naves
Gebreyes, Wondwossen Abebe
de Oliveira, Celso José Bruno
author_facet Silva, Gedean Galdino da Cruz
Campana, Eloiza Helena
Vasconcelos, Priscylla Carvalho
da Silva, Núbia Michelle Vieira
Santos Filho, Lauro
Leite, Elma Lima
Givisiez, Patrícia Emília Naves
Gebreyes, Wondwossen Abebe
de Oliveira, Celso José Bruno
author_sort Silva, Gedean Galdino da Cruz
collection PubMed
description The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene (bla(KPC)) in Psittaciformes rescued from wildlife trafficking in Paraíba State, Brazil. Cloacal swabs were collected from thirty birds and cultured by conventional microbiology using MacConkey and serum tryptone glucose glycerol (STGG) media supplemented with selective antimicrobials. E. coli isolates (n = 43) were identified by phenotypic tests and confirmed by MALDI-TOF. Antimicrobial susceptibility profiles were determined by means of Kirby–Bauer test. All isolates were further screened for extended-spectrum beta-lactamase (ESBL) production, and putative genes encoding ESBL were investigated by PCR. Additionally, bla(KPC)-harboring strains were genotyped by REP-PCR. A total of 43 E. coli phenotypically resistant isolates were recovered. The highest resistance rate was observed against ciprofloxacin. Among the resistance genes, only bla(KPC) was found in seven different birds from three species. According to the genotyping, these seven isolates belonged to four different strains. To date, this is the first report on the occurrence of KPC-E. coli in Psittaciformes rescued from trafficking in Northeastern Brazil. Due to the high clinical importance of KPC-E. coli, our findings suggest that wild animals in captivity at wildlife rescue centers can play a role as reservoirs of bacteria that are resistance to Critically Important antimicrobials in human medicine.
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spelling pubmed-77963782021-01-10 Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil Silva, Gedean Galdino da Cruz Campana, Eloiza Helena Vasconcelos, Priscylla Carvalho da Silva, Núbia Michelle Vieira Santos Filho, Lauro Leite, Elma Lima Givisiez, Patrícia Emília Naves Gebreyes, Wondwossen Abebe de Oliveira, Celso José Bruno Int J Environ Res Public Health Article The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene (bla(KPC)) in Psittaciformes rescued from wildlife trafficking in Paraíba State, Brazil. Cloacal swabs were collected from thirty birds and cultured by conventional microbiology using MacConkey and serum tryptone glucose glycerol (STGG) media supplemented with selective antimicrobials. E. coli isolates (n = 43) were identified by phenotypic tests and confirmed by MALDI-TOF. Antimicrobial susceptibility profiles were determined by means of Kirby–Bauer test. All isolates were further screened for extended-spectrum beta-lactamase (ESBL) production, and putative genes encoding ESBL were investigated by PCR. Additionally, bla(KPC)-harboring strains were genotyped by REP-PCR. A total of 43 E. coli phenotypically resistant isolates were recovered. The highest resistance rate was observed against ciprofloxacin. Among the resistance genes, only bla(KPC) was found in seven different birds from three species. According to the genotyping, these seven isolates belonged to four different strains. To date, this is the first report on the occurrence of KPC-E. coli in Psittaciformes rescued from trafficking in Northeastern Brazil. Due to the high clinical importance of KPC-E. coli, our findings suggest that wild animals in captivity at wildlife rescue centers can play a role as reservoirs of bacteria that are resistance to Critically Important antimicrobials in human medicine. MDPI 2020-12-25 2021-01 /pmc/articles/PMC7796378/ /pubmed/33375538 http://dx.doi.org/10.3390/ijerph18010095 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Gedean Galdino da Cruz
Campana, Eloiza Helena
Vasconcelos, Priscylla Carvalho
da Silva, Núbia Michelle Vieira
Santos Filho, Lauro
Leite, Elma Lima
Givisiez, Patrícia Emília Naves
Gebreyes, Wondwossen Abebe
de Oliveira, Celso José Bruno
Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil
title Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil
title_full Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil
title_fullStr Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil
title_full_unstemmed Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil
title_short Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil
title_sort occurrence of kpc-producing escherichia coli in psittaciformes rescued from trafficking in paraíba, brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796378/
https://www.ncbi.nlm.nih.gov/pubmed/33375538
http://dx.doi.org/10.3390/ijerph18010095
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