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New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features
Three novel Co(II) complexes of the type [Co(C(4)H(5)O(2))(2)L(2)] (where C(4)H(5)O(2) is methacrylate anion; L = C(3)H(4)N(2) (imidazole; HIm) (1), C(4)H(6)N(2) (2-methylimidazole; 2-MeIm) (2), C(5)H(8)N(2) (2-ethylimidazole; 2-EtIm) (3)) have been synthesized and characterized by elemental analysi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796409/ https://www.ncbi.nlm.nih.gov/pubmed/33374438 http://dx.doi.org/10.3390/molecules26010055 |
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author | Fudulu, Alina Olar, Rodica Maxim, Cătălin Scăeţeanu, Gina Vasile Bleotu, Coralia Matei, Lilia Chifiriuc, Mariana Carmen Badea, Mihaela |
author_facet | Fudulu, Alina Olar, Rodica Maxim, Cătălin Scăeţeanu, Gina Vasile Bleotu, Coralia Matei, Lilia Chifiriuc, Mariana Carmen Badea, Mihaela |
author_sort | Fudulu, Alina |
collection | PubMed |
description | Three novel Co(II) complexes of the type [Co(C(4)H(5)O(2))(2)L(2)] (where C(4)H(5)O(2) is methacrylate anion; L = C(3)H(4)N(2) (imidazole; HIm) (1), C(4)H(6)N(2) (2-methylimidazole; 2-MeIm) (2), C(5)H(8)N(2) (2-ethylimidazole; 2-EtIm) (3)) have been synthesized and characterized by elemental analysis, IR and UV-Vis spectroscopic techniques, thermal analysis and single crystal X-ray diffraction. X-ray crystallography revealed for complexes (1) and (2) distorted trigonal bipyramid stereochemistry for Co(II), meanwhile for complex (3) evidenced that the unit cell comprises three molecular units with interesting structural features. In each unit, both stereochemistry adopted by metallic ion and coordination modes of carboxylate anions are different. The screening of antimicrobial activity revealed that Candida albicans planktonic cells were the most susceptible, with minimal inhibitory concentration (MIC) values of 7.8 μg/mL for complexes (1) and (2) and 15.6 μg/mL for complex (3). Complexes (1) and (2) proved to be more active than complex (3) against the tested bacterial strains, both in planktonic and biofilm growth state, with MIC and minimal biofilm eradication concentration (MBEC) values ranging from 15.6 to 62.5 μg/mL, the best antibacterial effects being noticed against Staphylococcus aureus and Pseudomonas aeruginosa. Remarkably, the MBEC values obtained for the four tested bacterial strains were either identical or even lower than the MIC ones. The cytotoxicity assay indicated that the tested complexes affected the cellular cycle of HeLa, HCT-8, and MG63 cells, probably by inhibiting the expression of vimentin and transient receptor potential canonical 1 (TRPC1). The obtained biological results recommend these complexes as potential candidates for the development of novel anti-biofilm agents. |
format | Online Article Text |
id | pubmed-7796409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77964092021-01-10 New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features Fudulu, Alina Olar, Rodica Maxim, Cătălin Scăeţeanu, Gina Vasile Bleotu, Coralia Matei, Lilia Chifiriuc, Mariana Carmen Badea, Mihaela Molecules Article Three novel Co(II) complexes of the type [Co(C(4)H(5)O(2))(2)L(2)] (where C(4)H(5)O(2) is methacrylate anion; L = C(3)H(4)N(2) (imidazole; HIm) (1), C(4)H(6)N(2) (2-methylimidazole; 2-MeIm) (2), C(5)H(8)N(2) (2-ethylimidazole; 2-EtIm) (3)) have been synthesized and characterized by elemental analysis, IR and UV-Vis spectroscopic techniques, thermal analysis and single crystal X-ray diffraction. X-ray crystallography revealed for complexes (1) and (2) distorted trigonal bipyramid stereochemistry for Co(II), meanwhile for complex (3) evidenced that the unit cell comprises three molecular units with interesting structural features. In each unit, both stereochemistry adopted by metallic ion and coordination modes of carboxylate anions are different. The screening of antimicrobial activity revealed that Candida albicans planktonic cells were the most susceptible, with minimal inhibitory concentration (MIC) values of 7.8 μg/mL for complexes (1) and (2) and 15.6 μg/mL for complex (3). Complexes (1) and (2) proved to be more active than complex (3) against the tested bacterial strains, both in planktonic and biofilm growth state, with MIC and minimal biofilm eradication concentration (MBEC) values ranging from 15.6 to 62.5 μg/mL, the best antibacterial effects being noticed against Staphylococcus aureus and Pseudomonas aeruginosa. Remarkably, the MBEC values obtained for the four tested bacterial strains were either identical or even lower than the MIC ones. The cytotoxicity assay indicated that the tested complexes affected the cellular cycle of HeLa, HCT-8, and MG63 cells, probably by inhibiting the expression of vimentin and transient receptor potential canonical 1 (TRPC1). The obtained biological results recommend these complexes as potential candidates for the development of novel anti-biofilm agents. MDPI 2020-12-24 /pmc/articles/PMC7796409/ /pubmed/33374438 http://dx.doi.org/10.3390/molecules26010055 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fudulu, Alina Olar, Rodica Maxim, Cătălin Scăeţeanu, Gina Vasile Bleotu, Coralia Matei, Lilia Chifiriuc, Mariana Carmen Badea, Mihaela New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features |
title | New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features |
title_full | New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features |
title_fullStr | New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features |
title_full_unstemmed | New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features |
title_short | New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features |
title_sort | new cobalt (ii) complexes with imidazole derivatives: antimicrobial efficiency against planktonic and adherent microbes and in vitro cytotoxicity features |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796409/ https://www.ncbi.nlm.nih.gov/pubmed/33374438 http://dx.doi.org/10.3390/molecules26010055 |
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