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Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer

BACKGROUND: Novel and non-invasive biomarkers with higher sensitivity and specificity for the diagnosis of prostate cancer (PCa) is urgently needed. In this study, we used next-generation sequencing (NGS) to characterize the genome-wide exosomal miRNA expression profiling in urine specimens and expl...

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Autores principales: Li, Zhuo, Li, La-Xiu, Diao, Yan-Jun, Wang, Juan, Ye, Yun, Hao, Xiao-Ke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797303/
https://www.ncbi.nlm.nih.gov/pubmed/33442291
http://dx.doi.org/10.2147/CMAR.S272140
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author Li, Zhuo
Li, La-Xiu
Diao, Yan-Jun
Wang, Juan
Ye, Yun
Hao, Xiao-Ke
author_facet Li, Zhuo
Li, La-Xiu
Diao, Yan-Jun
Wang, Juan
Ye, Yun
Hao, Xiao-Ke
author_sort Li, Zhuo
collection PubMed
description BACKGROUND: Novel and non-invasive biomarkers with higher sensitivity and specificity for the diagnosis of prostate cancer (PCa) is urgently needed. In this study, we used next-generation sequencing (NGS) to characterize the genome-wide exosomal miRNA expression profiling in urine specimens and explored the diagnostic potential of urinary exosomal miRNAs for PCa. METHODS: Urinary exosomal microRNA expression profiling was performed by next-generation sequencing (NGS) and then validated by quantitative real-time PCR. RESULTS: Significant downregulation of urinary exosomal miR-375 was observed in PCa patients compared with healthy controls, while the expression levels of urinary exosomal miR-451a, miR-486-3p and miR-486-5p were found to be significantly up-regulated in the PCa patients. Furthermore, the expression level of urinary exosomal miR-375 showed a significant correlation with the clinical T-stage and bone metastasis of patients with PCa (P<0.05). Receiver operator characteristic curve demonstrated that the urinary exosomal miR-375, miR-451a, miR-486-3p and miR-486-5p levels can be used to differentiate PCa patients from healthy controls, with area under the curves (AUCs) of 0.788, 0.757, 0.704 and 0.796, respectively. The urinary exosomal miR-375 was found to be superior in discriminating between localized and metastatic PCa with an AUC of 0.806. Moreover, PCa patients can be distinguished from patients with benign prostatic hyperplasia by using a panel combining urinary exosomal miR-375 and miR-451a with an AUC of 0.726. CONCLUSION: These findings demonstrate that the urinary exosomal miRNAs can serve as novel and non-invasive biomarkers for diagnosing and predicting the progression of PCa.
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spelling pubmed-77973032021-01-12 Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer Li, Zhuo Li, La-Xiu Diao, Yan-Jun Wang, Juan Ye, Yun Hao, Xiao-Ke Cancer Manag Res Original Research BACKGROUND: Novel and non-invasive biomarkers with higher sensitivity and specificity for the diagnosis of prostate cancer (PCa) is urgently needed. In this study, we used next-generation sequencing (NGS) to characterize the genome-wide exosomal miRNA expression profiling in urine specimens and explored the diagnostic potential of urinary exosomal miRNAs for PCa. METHODS: Urinary exosomal microRNA expression profiling was performed by next-generation sequencing (NGS) and then validated by quantitative real-time PCR. RESULTS: Significant downregulation of urinary exosomal miR-375 was observed in PCa patients compared with healthy controls, while the expression levels of urinary exosomal miR-451a, miR-486-3p and miR-486-5p were found to be significantly up-regulated in the PCa patients. Furthermore, the expression level of urinary exosomal miR-375 showed a significant correlation with the clinical T-stage and bone metastasis of patients with PCa (P<0.05). Receiver operator characteristic curve demonstrated that the urinary exosomal miR-375, miR-451a, miR-486-3p and miR-486-5p levels can be used to differentiate PCa patients from healthy controls, with area under the curves (AUCs) of 0.788, 0.757, 0.704 and 0.796, respectively. The urinary exosomal miR-375 was found to be superior in discriminating between localized and metastatic PCa with an AUC of 0.806. Moreover, PCa patients can be distinguished from patients with benign prostatic hyperplasia by using a panel combining urinary exosomal miR-375 and miR-451a with an AUC of 0.726. CONCLUSION: These findings demonstrate that the urinary exosomal miRNAs can serve as novel and non-invasive biomarkers for diagnosing and predicting the progression of PCa. Dove 2021-01-06 /pmc/articles/PMC7797303/ /pubmed/33442291 http://dx.doi.org/10.2147/CMAR.S272140 Text en © 2021 Li et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Li, Zhuo
Li, La-Xiu
Diao, Yan-Jun
Wang, Juan
Ye, Yun
Hao, Xiao-Ke
Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer
title Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer
title_full Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer
title_fullStr Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer
title_full_unstemmed Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer
title_short Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer
title_sort identification of urinary exosomal mirnas for the non-invasive diagnosis of prostate cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797303/
https://www.ncbi.nlm.nih.gov/pubmed/33442291
http://dx.doi.org/10.2147/CMAR.S272140
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