Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test

Mitochondria play pivotal roles in cellular energy metabolism. Most of the intracellular adenosine triphosphate (ATP) is generated by mitochondrial respiration. The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line H...

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Detalles Bibliográficos
Autores principales: Gu, Xia, Ma, Yuan, Liu, Yi, Wan, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797920/
https://www.ncbi.nlm.nih.gov/pubmed/33458707
http://dx.doi.org/10.1016/j.xpro.2020.100245
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author Gu, Xia
Ma, Yuan
Liu, Yi
Wan, Qiang
author_facet Gu, Xia
Ma, Yuan
Liu, Yi
Wan, Qiang
author_sort Gu, Xia
collection PubMed
description Mitochondria play pivotal roles in cellular energy metabolism. Most of the intracellular adenosine triphosphate (ATP) is generated by mitochondrial respiration. The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an example to present the procedures to quantify the oxygen consumption rate using a Seahorse XFe96 extracellular flux analyzer. For complete details on the use and execution of this protocol, please refer to Ma et al. (2020).
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spelling pubmed-77979202021-01-15 Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test Gu, Xia Ma, Yuan Liu, Yi Wan, Qiang STAR Protoc Protocol Mitochondria play pivotal roles in cellular energy metabolism. Most of the intracellular adenosine triphosphate (ATP) is generated by mitochondrial respiration. The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an example to present the procedures to quantify the oxygen consumption rate using a Seahorse XFe96 extracellular flux analyzer. For complete details on the use and execution of this protocol, please refer to Ma et al. (2020). Elsevier 2020-12-30 /pmc/articles/PMC7797920/ /pubmed/33458707 http://dx.doi.org/10.1016/j.xpro.2020.100245 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gu, Xia
Ma, Yuan
Liu, Yi
Wan, Qiang
Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test
title Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test
title_full Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test
title_fullStr Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test
title_full_unstemmed Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test
title_short Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test
title_sort measurement of mitochondrial respiration in adherent cells by seahorse xf96 cell mito stress test
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797920/
https://www.ncbi.nlm.nih.gov/pubmed/33458707
http://dx.doi.org/10.1016/j.xpro.2020.100245
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