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Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This pro...

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Detalles Bibliográficos
Autores principales: Toda, Gotaro, Yamauchi, Toshimasa, Kadowaki, Takashi, Ueki, Kohjiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797923/
https://www.ncbi.nlm.nih.gov/pubmed/33458708
http://dx.doi.org/10.1016/j.xpro.2020.100246
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author Toda, Gotaro
Yamauchi, Toshimasa
Kadowaki, Takashi
Ueki, Kohjiro
author_facet Toda, Gotaro
Yamauchi, Toshimasa
Kadowaki, Takashi
Ueki, Kohjiro
author_sort Toda, Gotaro
collection PubMed
description The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020).
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spelling pubmed-77979232021-01-15 Preparation and culture of bone marrow-derived macrophages from mice for functional analysis Toda, Gotaro Yamauchi, Toshimasa Kadowaki, Takashi Ueki, Kohjiro STAR Protoc Protocol The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020). Elsevier 2020-12-31 /pmc/articles/PMC7797923/ /pubmed/33458708 http://dx.doi.org/10.1016/j.xpro.2020.100246 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Toda, Gotaro
Yamauchi, Toshimasa
Kadowaki, Takashi
Ueki, Kohjiro
Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
title Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
title_full Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
title_fullStr Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
title_full_unstemmed Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
title_short Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
title_sort preparation and culture of bone marrow-derived macrophages from mice for functional analysis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797923/
https://www.ncbi.nlm.nih.gov/pubmed/33458708
http://dx.doi.org/10.1016/j.xpro.2020.100246
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