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Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This pro...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797923/ https://www.ncbi.nlm.nih.gov/pubmed/33458708 http://dx.doi.org/10.1016/j.xpro.2020.100246 |
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author | Toda, Gotaro Yamauchi, Toshimasa Kadowaki, Takashi Ueki, Kohjiro |
author_facet | Toda, Gotaro Yamauchi, Toshimasa Kadowaki, Takashi Ueki, Kohjiro |
author_sort | Toda, Gotaro |
collection | PubMed |
description | The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020). |
format | Online Article Text |
id | pubmed-7797923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77979232021-01-15 Preparation and culture of bone marrow-derived macrophages from mice for functional analysis Toda, Gotaro Yamauchi, Toshimasa Kadowaki, Takashi Ueki, Kohjiro STAR Protoc Protocol The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020). Elsevier 2020-12-31 /pmc/articles/PMC7797923/ /pubmed/33458708 http://dx.doi.org/10.1016/j.xpro.2020.100246 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Toda, Gotaro Yamauchi, Toshimasa Kadowaki, Takashi Ueki, Kohjiro Preparation and culture of bone marrow-derived macrophages from mice for functional analysis |
title | Preparation and culture of bone marrow-derived macrophages from mice for functional analysis |
title_full | Preparation and culture of bone marrow-derived macrophages from mice for functional analysis |
title_fullStr | Preparation and culture of bone marrow-derived macrophages from mice for functional analysis |
title_full_unstemmed | Preparation and culture of bone marrow-derived macrophages from mice for functional analysis |
title_short | Preparation and culture of bone marrow-derived macrophages from mice for functional analysis |
title_sort | preparation and culture of bone marrow-derived macrophages from mice for functional analysis |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797923/ https://www.ncbi.nlm.nih.gov/pubmed/33458708 http://dx.doi.org/10.1016/j.xpro.2020.100246 |
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