Calcium imaging of adult-born neurons in freely moving mice

Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca(2+) imaging of ABN activity using a miniaturized fluorescent mi...

Descripción completa

Detalles Bibliográficos
Autores principales: Carrier-Ruiz, Alvaro, Sugaya, Yuki, Kumar, Deependra, Vergara, Pablo, Koyanagi, Iyo, Srinivasan, Sakthivel, Naoi, Toshie, Kano, Masanobu, Sakaguchi, Masanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797924/
https://www.ncbi.nlm.nih.gov/pubmed/33458703
http://dx.doi.org/10.1016/j.xpro.2020.100238
Descripción
Sumario:Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca(2+) imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).

Ejemplares similares