Calcium imaging of adult-born neurons in freely moving mice

Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca(2+) imaging of ABN activity using a miniaturized fluorescent mi...

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Autores principales: Carrier-Ruiz, Alvaro, Sugaya, Yuki, Kumar, Deependra, Vergara, Pablo, Koyanagi, Iyo, Srinivasan, Sakthivel, Naoi, Toshie, Kano, Masanobu, Sakaguchi, Masanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797924/
https://www.ncbi.nlm.nih.gov/pubmed/33458703
http://dx.doi.org/10.1016/j.xpro.2020.100238
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author Carrier-Ruiz, Alvaro
Sugaya, Yuki
Kumar, Deependra
Vergara, Pablo
Koyanagi, Iyo
Srinivasan, Sakthivel
Naoi, Toshie
Kano, Masanobu
Sakaguchi, Masanori
author_facet Carrier-Ruiz, Alvaro
Sugaya, Yuki
Kumar, Deependra
Vergara, Pablo
Koyanagi, Iyo
Srinivasan, Sakthivel
Naoi, Toshie
Kano, Masanobu
Sakaguchi, Masanori
author_sort Carrier-Ruiz, Alvaro
collection PubMed
description Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca(2+) imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).
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spelling pubmed-77979242021-01-15 Calcium imaging of adult-born neurons in freely moving mice Carrier-Ruiz, Alvaro Sugaya, Yuki Kumar, Deependra Vergara, Pablo Koyanagi, Iyo Srinivasan, Sakthivel Naoi, Toshie Kano, Masanobu Sakaguchi, Masanori STAR Protoc Protocol Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca(2+) imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020). Elsevier 2020-12-31 /pmc/articles/PMC7797924/ /pubmed/33458703 http://dx.doi.org/10.1016/j.xpro.2020.100238 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Carrier-Ruiz, Alvaro
Sugaya, Yuki
Kumar, Deependra
Vergara, Pablo
Koyanagi, Iyo
Srinivasan, Sakthivel
Naoi, Toshie
Kano, Masanobu
Sakaguchi, Masanori
Calcium imaging of adult-born neurons in freely moving mice
title Calcium imaging of adult-born neurons in freely moving mice
title_full Calcium imaging of adult-born neurons in freely moving mice
title_fullStr Calcium imaging of adult-born neurons in freely moving mice
title_full_unstemmed Calcium imaging of adult-born neurons in freely moving mice
title_short Calcium imaging of adult-born neurons in freely moving mice
title_sort calcium imaging of adult-born neurons in freely moving mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797924/
https://www.ncbi.nlm.nih.gov/pubmed/33458703
http://dx.doi.org/10.1016/j.xpro.2020.100238
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