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Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth

Pseudomonas aeruginosa is an environmental bacterium and an opportunistic human pathogen. It is also a well-established model organism to study bacterial adaptation to stressful conditions, such as those encountered during an infection process in the human host. Advancing knowledge on P. aeruginosa...

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Autores principales: Schinner, Silvia, Engelhardt, Florian, Preusse, Matthias, Thöming, Janne Gesine, Tomasch, Jürgen, Häussler, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798452/
https://www.ncbi.nlm.nih.gov/pubmed/33447809
http://dx.doi.org/10.1016/j.bioflm.2020.100023
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author Schinner, Silvia
Engelhardt, Florian
Preusse, Matthias
Thöming, Janne Gesine
Tomasch, Jürgen
Häussler, Susanne
author_facet Schinner, Silvia
Engelhardt, Florian
Preusse, Matthias
Thöming, Janne Gesine
Tomasch, Jürgen
Häussler, Susanne
author_sort Schinner, Silvia
collection PubMed
description Pseudomonas aeruginosa is an environmental bacterium and an opportunistic human pathogen. It is also a well-established model organism to study bacterial adaptation to stressful conditions, such as those encountered during an infection process in the human host. Advancing knowledge on P. aeruginosa adaptation to biofilm growth conditions is bound to reveal novel strategies and targets for the treatment of chronic biofilm-associated infections. Here, we generated transposon insertion libraries in three P. aeruginosa strain backgrounds and determined the relative frequency of each insertion following biofilm growth using transposon sequencing. We demonstrate that in general the SOS response, several tRNA modifying enzymes as well as adaptation to microaerophilic growth conditions play a key role in bacterial survival under biofilm growth conditions. On the other hand, presence of genes involved in motility and PQS signaling were less important during biofilm growth. Several mutants exhibiting transposon insertions in genes detected in our screen were validated for their biofilm growth capabilities and biofilm specific transcriptional responses using independently generated transposon mutants. Our results provide new insights into P. aeruginosa adaptation to biofilm growth conditions. The detection of previously unknown determinants of biofilm survival supports the use of transposon insertion sequencing as a global genomic technology for understanding the establishment of difficult to treat biofilm-associated infections.
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spelling pubmed-77984522021-01-13 Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth Schinner, Silvia Engelhardt, Florian Preusse, Matthias Thöming, Janne Gesine Tomasch, Jürgen Häussler, Susanne Biofilm Article Pseudomonas aeruginosa is an environmental bacterium and an opportunistic human pathogen. It is also a well-established model organism to study bacterial adaptation to stressful conditions, such as those encountered during an infection process in the human host. Advancing knowledge on P. aeruginosa adaptation to biofilm growth conditions is bound to reveal novel strategies and targets for the treatment of chronic biofilm-associated infections. Here, we generated transposon insertion libraries in three P. aeruginosa strain backgrounds and determined the relative frequency of each insertion following biofilm growth using transposon sequencing. We demonstrate that in general the SOS response, several tRNA modifying enzymes as well as adaptation to microaerophilic growth conditions play a key role in bacterial survival under biofilm growth conditions. On the other hand, presence of genes involved in motility and PQS signaling were less important during biofilm growth. Several mutants exhibiting transposon insertions in genes detected in our screen were validated for their biofilm growth capabilities and biofilm specific transcriptional responses using independently generated transposon mutants. Our results provide new insights into P. aeruginosa adaptation to biofilm growth conditions. The detection of previously unknown determinants of biofilm survival supports the use of transposon insertion sequencing as a global genomic technology for understanding the establishment of difficult to treat biofilm-associated infections. Elsevier 2020-04-02 /pmc/articles/PMC7798452/ /pubmed/33447809 http://dx.doi.org/10.1016/j.bioflm.2020.100023 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Schinner, Silvia
Engelhardt, Florian
Preusse, Matthias
Thöming, Janne Gesine
Tomasch, Jürgen
Häussler, Susanne
Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth
title Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth
title_full Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth
title_fullStr Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth
title_full_unstemmed Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth
title_short Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth
title_sort genetic determinants of pseudomonas aeruginosa fitness during biofilm growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798452/
https://www.ncbi.nlm.nih.gov/pubmed/33447809
http://dx.doi.org/10.1016/j.bioflm.2020.100023
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