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Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants
BACKGROUND: Human spillovers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to dogs and the emergence of a highly contagious avian-origin H3N2 canine influenza virus have raised concerns on the role of dogs in the spread of SARS-CoV-2 and their susceptibility to existing human and a...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799041/ https://www.ncbi.nlm.nih.gov/pubmed/33395484 http://dx.doi.org/10.1093/infdis/jiab002 |
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author | Bui, Christine H T Yeung, Hin Wo Ho, John C W Leung, Connie Y H Hui, Kenrie P Y Perera, Ranawaka A P M Webby, Richard J Schultz-Cherry, Stacey L Nicholls, John M Peiris, Joseph Sriyal Malik Chan, Michael C W |
author_facet | Bui, Christine H T Yeung, Hin Wo Ho, John C W Leung, Connie Y H Hui, Kenrie P Y Perera, Ranawaka A P M Webby, Richard J Schultz-Cherry, Stacey L Nicholls, John M Peiris, Joseph Sriyal Malik Chan, Michael C W |
author_sort | Bui, Christine H T |
collection | PubMed |
description | BACKGROUND: Human spillovers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to dogs and the emergence of a highly contagious avian-origin H3N2 canine influenza virus have raised concerns on the role of dogs in the spread of SARS-CoV-2 and their susceptibility to existing human and avian influenza viruses, which might result in further reassortment. METHODS: We systematically studied the replication kinetics of SARS-CoV-2, SARS-CoV, influenza A viruses of H1, H3, H5, H7, and H9 subtypes, and influenza B viruses of Yamagata-like and Victoria-like lineages in ex vivo canine nasal cavity, soft palate, trachea, and lung tissue explant cultures and examined ACE2 and sialic acid (SA) receptor distribution in these tissues. RESULTS: There was limited productive replication of SARS-CoV-2 in canine nasal cavity and SARS-CoV in canine nasal cavity, soft palate, and lung, with unexpectedly high ACE2 levels in canine nasal cavity and soft palate. Canine tissues were susceptible to a wide range of human and avian influenza viruses, which matched with the abundance of both human and avian SA receptors. CONCLUSIONS: Existence of suitable receptors and tropism for the same tissue foster virus adaptation and reassortment. Continuous surveillance in dog populations should be conducted given the many chances for spillover during outbreaks. |
format | Online Article Text |
id | pubmed-7799041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-77990412021-01-25 Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants Bui, Christine H T Yeung, Hin Wo Ho, John C W Leung, Connie Y H Hui, Kenrie P Y Perera, Ranawaka A P M Webby, Richard J Schultz-Cherry, Stacey L Nicholls, John M Peiris, Joseph Sriyal Malik Chan, Michael C W J Infect Dis Major Articles and Brief Reports BACKGROUND: Human spillovers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to dogs and the emergence of a highly contagious avian-origin H3N2 canine influenza virus have raised concerns on the role of dogs in the spread of SARS-CoV-2 and their susceptibility to existing human and avian influenza viruses, which might result in further reassortment. METHODS: We systematically studied the replication kinetics of SARS-CoV-2, SARS-CoV, influenza A viruses of H1, H3, H5, H7, and H9 subtypes, and influenza B viruses of Yamagata-like and Victoria-like lineages in ex vivo canine nasal cavity, soft palate, trachea, and lung tissue explant cultures and examined ACE2 and sialic acid (SA) receptor distribution in these tissues. RESULTS: There was limited productive replication of SARS-CoV-2 in canine nasal cavity and SARS-CoV in canine nasal cavity, soft palate, and lung, with unexpectedly high ACE2 levels in canine nasal cavity and soft palate. Canine tissues were susceptible to a wide range of human and avian influenza viruses, which matched with the abundance of both human and avian SA receptors. CONCLUSIONS: Existence of suitable receptors and tropism for the same tissue foster virus adaptation and reassortment. Continuous surveillance in dog populations should be conducted given the many chances for spillover during outbreaks. Oxford University Press 2021-01-04 /pmc/articles/PMC7799041/ /pubmed/33395484 http://dx.doi.org/10.1093/infdis/jiab002 Text en © The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com. https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_modelThis article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) |
spellingShingle | Major Articles and Brief Reports Bui, Christine H T Yeung, Hin Wo Ho, John C W Leung, Connie Y H Hui, Kenrie P Y Perera, Ranawaka A P M Webby, Richard J Schultz-Cherry, Stacey L Nicholls, John M Peiris, Joseph Sriyal Malik Chan, Michael C W Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants |
title | Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants |
title_full | Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants |
title_fullStr | Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants |
title_full_unstemmed | Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants |
title_short | Tropism of SARS-CoV-2, SARS-CoV, and Influenza Virus in Canine Tissue Explants |
title_sort | tropism of sars-cov-2, sars-cov, and influenza virus in canine tissue explants |
topic | Major Articles and Brief Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799041/ https://www.ncbi.nlm.nih.gov/pubmed/33395484 http://dx.doi.org/10.1093/infdis/jiab002 |
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