Time-resolved cryoEM using Spotiton

We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified...

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Autores principales: Dandey, Venkata P., Budell, William C., Wei, Hui, Bobe, Daija, Maruthi, Kashyap, Kopylov, Mykhailo, Eng, Edward T., Kahn, Peter A., Hinshaw, Jenny E., Kundu, Nidhi, Nimigean, Crina M., Fan, Chen, Sukomon, Nattakan, Darst, Seth A., Saecker, Ruth M., Chen, James, Malone, Brandon, Potter, Clinton S., Carragher, Bridget
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799389/
https://www.ncbi.nlm.nih.gov/pubmed/32778833
http://dx.doi.org/10.1038/s41592-020-0925-6
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author Dandey, Venkata P.
Budell, William C.
Wei, Hui
Bobe, Daija
Maruthi, Kashyap
Kopylov, Mykhailo
Eng, Edward T.
Kahn, Peter A.
Hinshaw, Jenny E.
Kundu, Nidhi
Nimigean, Crina M.
Fan, Chen
Sukomon, Nattakan
Darst, Seth A.
Saecker, Ruth M.
Chen, James
Malone, Brandon
Potter, Clinton S.
Carragher, Bridget
author_facet Dandey, Venkata P.
Budell, William C.
Wei, Hui
Bobe, Daija
Maruthi, Kashyap
Kopylov, Mykhailo
Eng, Edward T.
Kahn, Peter A.
Hinshaw, Jenny E.
Kundu, Nidhi
Nimigean, Crina M.
Fan, Chen
Sukomon, Nattakan
Darst, Seth A.
Saecker, Ruth M.
Chen, James
Malone, Brandon
Potter, Clinton S.
Carragher, Bridget
author_sort Dandey, Venkata P.
collection PubMed
description We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified in liquid ethane, ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.
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spelling pubmed-77993892021-02-10 Time-resolved cryoEM using Spotiton Dandey, Venkata P. Budell, William C. Wei, Hui Bobe, Daija Maruthi, Kashyap Kopylov, Mykhailo Eng, Edward T. Kahn, Peter A. Hinshaw, Jenny E. Kundu, Nidhi Nimigean, Crina M. Fan, Chen Sukomon, Nattakan Darst, Seth A. Saecker, Ruth M. Chen, James Malone, Brandon Potter, Clinton S. Carragher, Bridget Nat Methods Article We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified in liquid ethane, ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest. 2020-08-10 2020-09 /pmc/articles/PMC7799389/ /pubmed/32778833 http://dx.doi.org/10.1038/s41592-020-0925-6 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Dandey, Venkata P.
Budell, William C.
Wei, Hui
Bobe, Daija
Maruthi, Kashyap
Kopylov, Mykhailo
Eng, Edward T.
Kahn, Peter A.
Hinshaw, Jenny E.
Kundu, Nidhi
Nimigean, Crina M.
Fan, Chen
Sukomon, Nattakan
Darst, Seth A.
Saecker, Ruth M.
Chen, James
Malone, Brandon
Potter, Clinton S.
Carragher, Bridget
Time-resolved cryoEM using Spotiton
title Time-resolved cryoEM using Spotiton
title_full Time-resolved cryoEM using Spotiton
title_fullStr Time-resolved cryoEM using Spotiton
title_full_unstemmed Time-resolved cryoEM using Spotiton
title_short Time-resolved cryoEM using Spotiton
title_sort time-resolved cryoem using spotiton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799389/
https://www.ncbi.nlm.nih.gov/pubmed/32778833
http://dx.doi.org/10.1038/s41592-020-0925-6
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