Time-resolved cryoEM using Spotiton

We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified...

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Detalles Bibliográficos
Autores principales: Dandey, Venkata P., Budell, William C., Wei, Hui, Bobe, Daija, Maruthi, Kashyap, Kopylov, Mykhailo, Eng, Edward T., Kahn, Peter A., Hinshaw, Jenny E., Kundu, Nidhi, Nimigean, Crina M., Fan, Chen, Sukomon, Nattakan, Darst, Seth A., Saecker, Ruth M., Chen, James, Malone, Brandon, Potter, Clinton S., Carragher, Bridget
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799389/
https://www.ncbi.nlm.nih.gov/pubmed/32778833
http://dx.doi.org/10.1038/s41592-020-0925-6

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