Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection

Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction (qPCR) assay was established to detect FBoV-1. The melting curve showed a single melting peak at 83.0 ℃. The results of sensitivity showed that the detectio...

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Autores principales: Wang, Yong, Li, Wei, Guo, Xu, Zhang, Da, Sun, Jianfei, Fu, Ziteng, Liu, Guangqing, Li, Yongdong, Jiang, Shudong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Short Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799429/
https://www.ncbi.nlm.nih.gov/pubmed/33457175
http://dx.doi.org/10.1007/s13205-020-02577-8
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author Wang, Yong
Li, Wei
Guo, Xu
Zhang, Da
Sun, Jianfei
Fu, Ziteng
Liu, Guangqing
Li, Yongdong
Jiang, Shudong
author_facet Wang, Yong
Li, Wei
Guo, Xu
Zhang, Da
Sun, Jianfei
Fu, Ziteng
Liu, Guangqing
Li, Yongdong
Jiang, Shudong
author_sort Wang, Yong
collection PubMed
description Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction (qPCR) assay was established to detect FBoV-1. The melting curve showed a single melting peak at 83.0 ℃. The results of sensitivity showed that the detection limit of the qPCR was 3.87 × 10(1) copies/μL. Of note, the detection limit of the conventional polymerase chain reaction (cPCR) was 3.87 × 10(3) copies/μL. The highest intra-assay and inter-assay coefficients of variation (CV%) were 0.98% and 1.42%, respectively. The positive detection rate of 128 clinical samples using the qPCR and the cPCR was 7.0% (9/128) and 4.7% (6/128), respectively. Taken together, these results indicated that the established qPCR assay has good sensitivity, high specificity, and good reproducibility. Therefore, it could provide support for the rapid and efficient clinical detection of FBoV-1.
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spelling pubmed-77994292021-01-12 Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection Wang, Yong Li, Wei Guo, Xu Zhang, Da Sun, Jianfei Fu, Ziteng Liu, Guangqing Li, Yongdong Jiang, Shudong 3 Biotech Short Reports Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction (qPCR) assay was established to detect FBoV-1. The melting curve showed a single melting peak at 83.0 ℃. The results of sensitivity showed that the detection limit of the qPCR was 3.87 × 10(1) copies/μL. Of note, the detection limit of the conventional polymerase chain reaction (cPCR) was 3.87 × 10(3) copies/μL. The highest intra-assay and inter-assay coefficients of variation (CV%) were 0.98% and 1.42%, respectively. The positive detection rate of 128 clinical samples using the qPCR and the cPCR was 7.0% (9/128) and 4.7% (6/128), respectively. Taken together, these results indicated that the established qPCR assay has good sensitivity, high specificity, and good reproducibility. Therefore, it could provide support for the rapid and efficient clinical detection of FBoV-1. Springer International Publishing 2021-01-11 2021-02 /pmc/articles/PMC7799429/ /pubmed/33457175 http://dx.doi.org/10.1007/s13205-020-02577-8 Text en © King Abdulaziz City for Science and Technology 2021
spellingShingle Short Reports
Wang, Yong
Li, Wei
Guo, Xu
Zhang, Da
Sun, Jianfei
Fu, Ziteng
Liu, Guangqing
Li, Yongdong
Jiang, Shudong
Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
title Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
title_full Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
title_fullStr Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
title_full_unstemmed Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
title_short Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
title_sort development of sybr green i-based polymerase chain reaction for feline bocavirus 1 detection
topic Short Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799429/
https://www.ncbi.nlm.nih.gov/pubmed/33457175
http://dx.doi.org/10.1007/s13205-020-02577-8
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