Next-generation sequencing yields the complete mitochondrial genome of the Shangrila hot-spring snakes (Thermophis shangrila; Reptilia: Colubridae)

In this study, we sequenced the complete mitochondrial genome of Thermophis shangrila by using the next-generation sequencing technique. The total length of the mitogenome was 17,407 bp, which was composed of 13 protein coding genes, two rRNA genes (12s and 16s rRNA), 22 tRNA genes, and two control...

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Autores principales: Wu, Wei, Jiang, De-Chun, Sun, Feng-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Mitogenome Announcement
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799666/
https://www.ncbi.nlm.nih.gov/pubmed/33473816
http://dx.doi.org/10.1080/23802359.2017.1331330
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author Wu, Wei
Jiang, De-Chun
Sun, Feng-Hui
author_facet Wu, Wei
Jiang, De-Chun
Sun, Feng-Hui
author_sort Wu, Wei
collection PubMed
description In this study, we sequenced the complete mitochondrial genome of Thermophis shangrila by using the next-generation sequencing technique. The total length of the mitogenome was 17,407 bp, which was composed of 13 protein coding genes, two rRNA genes (12s and 16s rRNA), 22 tRNA genes, and two control regions (CRI and CRII). The base composition was 32.6% for A, 23.9% for T, 30.0% for C, and 13.5% for G. We added a fragment about 150 bp in length at control region I, which Peng et al. failed to obtain using Sanger dideoxy sequencing.
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spelling pubmed-77996662021-01-19 Next-generation sequencing yields the complete mitochondrial genome of the Shangrila hot-spring snakes (Thermophis shangrila; Reptilia: Colubridae) Wu, Wei Jiang, De-Chun Sun, Feng-Hui Mitochondrial DNA B Resour Mitogenome Announcement In this study, we sequenced the complete mitochondrial genome of Thermophis shangrila by using the next-generation sequencing technique. The total length of the mitogenome was 17,407 bp, which was composed of 13 protein coding genes, two rRNA genes (12s and 16s rRNA), 22 tRNA genes, and two control regions (CRI and CRII). The base composition was 32.6% for A, 23.9% for T, 30.0% for C, and 13.5% for G. We added a fragment about 150 bp in length at control region I, which Peng et al. failed to obtain using Sanger dideoxy sequencing. Taylor & Francis 2017-05-30 /pmc/articles/PMC7799666/ /pubmed/33473816 http://dx.doi.org/10.1080/23802359.2017.1331330 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Mitogenome Announcement
Wu, Wei
Jiang, De-Chun
Sun, Feng-Hui
Next-generation sequencing yields the complete mitochondrial genome of the Shangrila hot-spring snakes (Thermophis shangrila; Reptilia: Colubridae)
title Next-generation sequencing yields the complete mitochondrial genome of the Shangrila hot-spring snakes (Thermophis shangrila; Reptilia: Colubridae)
title_full Next-generation sequencing yields the complete mitochondrial genome of the Shangrila hot-spring snakes (Thermophis shangrila; Reptilia: Colubridae)
title_fullStr Next-generation sequencing yields the complete mitochondrial genome of the Shangrila hot-spring snakes (Thermophis shangrila; Reptilia: Colubridae)
title_full_unstemmed Next-generation sequencing yields the complete mitochondrial genome of the Shangrila hot-spring snakes (Thermophis shangrila; Reptilia: Colubridae)
title_short Next-generation sequencing yields the complete mitochondrial genome of the Shangrila hot-spring snakes (Thermophis shangrila; Reptilia: Colubridae)
title_sort next-generation sequencing yields the complete mitochondrial genome of the shangrila hot-spring snakes (thermophis shangrila; reptilia: colubridae)
topic Mitogenome Announcement
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799666/
https://www.ncbi.nlm.nih.gov/pubmed/33473816
http://dx.doi.org/10.1080/23802359.2017.1331330
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