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CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies

Insecticide resistance is a worldwide threat for vector control around the world, and Aedes aegypti, the main vector of several arboviruses, is a particular concern. To better understand the mechanisms of resistance, four isofemale strains originally from French Guiana were isolated and analysed usi...

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Autores principales: Epelboin, Yanouk, Wang, Lanjiao, Giai Gianetto, Quentin, Choumet, Valérie, Gaborit, Pascal, Issaly, Jean, Guidez, Amandine, Douché, Thibaut, Chaze, Thibault, Matondo, Mariette, Dusfour, Isabelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799788/
https://www.ncbi.nlm.nih.gov/pubmed/33428654
http://dx.doi.org/10.1371/journal.pone.0243992
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author Epelboin, Yanouk
Wang, Lanjiao
Giai Gianetto, Quentin
Choumet, Valérie
Gaborit, Pascal
Issaly, Jean
Guidez, Amandine
Douché, Thibaut
Chaze, Thibault
Matondo, Mariette
Dusfour, Isabelle
author_facet Epelboin, Yanouk
Wang, Lanjiao
Giai Gianetto, Quentin
Choumet, Valérie
Gaborit, Pascal
Issaly, Jean
Guidez, Amandine
Douché, Thibaut
Chaze, Thibault
Matondo, Mariette
Dusfour, Isabelle
author_sort Epelboin, Yanouk
collection PubMed
description Insecticide resistance is a worldwide threat for vector control around the world, and Aedes aegypti, the main vector of several arboviruses, is a particular concern. To better understand the mechanisms of resistance, four isofemale strains originally from French Guiana were isolated and analysed using combined approaches. The activity of detoxification enzymes involved in insecticide resistance was assayed, and mutations located at positions 1016 and 1534 of the sodium voltage-gated channel gene, which have been associated with pyrethroid resistance in Aedes aegypti populations in Latin America, were monitored. Resistance to other insecticide families (organophosphates and carbamates) was evaluated. A large-scale proteomic analysis was performed to identify proteins involved in insecticide resistance. Our results revealed a metabolic resistance and resistance associated with a mutation of the sodium voltage-gated channel gene at position 1016. Metabolic resistance was mediated through an increase of esterase activity in most strains but also through the shifts in the abundance of several cytochrome P450 (CYP450s). Overall, resistance to deltamethrin was linked in the isofemale strains to resistance to other class of insecticides, suggesting that cross- and multiple resistance occur through selection of mechanisms of metabolic resistance. These results give some insights into resistance to deltamethrin and into multiple resistance phenomena in populations of Ae. aegypti.
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spelling pubmed-77997882021-01-22 CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies Epelboin, Yanouk Wang, Lanjiao Giai Gianetto, Quentin Choumet, Valérie Gaborit, Pascal Issaly, Jean Guidez, Amandine Douché, Thibaut Chaze, Thibault Matondo, Mariette Dusfour, Isabelle PLoS One Research Article Insecticide resistance is a worldwide threat for vector control around the world, and Aedes aegypti, the main vector of several arboviruses, is a particular concern. To better understand the mechanisms of resistance, four isofemale strains originally from French Guiana were isolated and analysed using combined approaches. The activity of detoxification enzymes involved in insecticide resistance was assayed, and mutations located at positions 1016 and 1534 of the sodium voltage-gated channel gene, which have been associated with pyrethroid resistance in Aedes aegypti populations in Latin America, were monitored. Resistance to other insecticide families (organophosphates and carbamates) was evaluated. A large-scale proteomic analysis was performed to identify proteins involved in insecticide resistance. Our results revealed a metabolic resistance and resistance associated with a mutation of the sodium voltage-gated channel gene at position 1016. Metabolic resistance was mediated through an increase of esterase activity in most strains but also through the shifts in the abundance of several cytochrome P450 (CYP450s). Overall, resistance to deltamethrin was linked in the isofemale strains to resistance to other class of insecticides, suggesting that cross- and multiple resistance occur through selection of mechanisms of metabolic resistance. These results give some insights into resistance to deltamethrin and into multiple resistance phenomena in populations of Ae. aegypti. Public Library of Science 2021-01-11 /pmc/articles/PMC7799788/ /pubmed/33428654 http://dx.doi.org/10.1371/journal.pone.0243992 Text en © 2021 Epelboin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Epelboin, Yanouk
Wang, Lanjiao
Giai Gianetto, Quentin
Choumet, Valérie
Gaborit, Pascal
Issaly, Jean
Guidez, Amandine
Douché, Thibaut
Chaze, Thibault
Matondo, Mariette
Dusfour, Isabelle
CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies
title CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies
title_full CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies
title_fullStr CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies
title_full_unstemmed CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies
title_short CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies
title_sort cyp450 core involvement in multiple resistance strains of aedes aegypti from french guiana highlighted by proteomics, molecular and biochemical studies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799788/
https://www.ncbi.nlm.nih.gov/pubmed/33428654
http://dx.doi.org/10.1371/journal.pone.0243992
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